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对2009年分离自云南省昆明市脑膜脑炎儿童的1株柯萨奇B组1型病毒MSH-KM9-09,进行了全基因组的序列测定,并对其分子变异和进化特点进行了分析。采用逆转录-聚合酶链反应(Reverse transcription-polymerasechain reaction,RT-PCR),分段扩增覆盖病毒全长序列的8个重叠片段(未包括多聚腺苷酸尾),并进行序列测定和分析。CVB1MSH-KM9-09病毒株基因组全长7 384个核苷酸(nt),5'端和为3'端分别为741nt和94nt的非编码区,5'和3'非编码区间为一个6 549nt长的开放阅读框,编码一个含2 183个氨基酸的多聚蛋白,编码区内未见核苷酸的插入或缺失。与GenBank库中现有CVB1全基因组序列M16560、pmMC、Tucson B1和CVB1/Nm相比对,整个基因组的核苷酸同源性分别为80.9%、81.6%、80.5%和80%,氨基酸同源性分别为95.6%、95.8%、96.2%和95.6%。首次完成中国CVB1病毒全基因组核苷酸序列的测定,经核苷酸序列同源性比对和进化树分析,证实MSH/KM9/2009株属于CVB1基因型。
Abstract：To characterize the complete genome sequence of coxsackievirus B1(CVB1) MSH/KM9/2009 strain isolated from Yunnan,China,2009.Eight overlapping clones covering the whole viral genome(excluding the poly-A tail) were obtained by RT-PCR and sequenced,and their nucleotide and amino acid sequences were compared with other known CVB1 strains.The genome of the CVB1 MSH/KM9/2009 strain had 7384 nucleotides in length,and contained a 741nt non-translated region(NTR) at the 5′end and a 94nt NTR at the 3′end.The entire open reading frame contained 6 549 nt,encoding a 2 183-aa polyprotein.In the coding region,there was no nucleotide deletion or insertion,but some changes of amino acid were unique.The complete genome sequence alignments showed that the CVB1 isolate MSH/KM9/2009 strain shared the highest nucleotide(80.9%,81.6%,80.5% and 80%) and amino acid(95.6%,95.8%,96.2% and 95.6%) identities to the CVB1 M16560,pmMC,Tucson B1 and CVB1Nm strain,respectively.Phylogenetic tree analysis showed that the MSH/KM9/2009,CVB1 M16560,pmMC,Tucson B1 and CVB1Nm strain clustered into same group.The newly isolated CVB1 strain MSH/KM9/2009 from Yunnan Province belonged to genotype CVB1.
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基本信息:
DOI：10.13242/j.cnki.bingduxuebao.002389
中图分类号:S852.65
引用信息:
[1]刘建生,邵聪文,潘玥,等.柯萨奇B组1型云南分离株MSH/KM9/2009全基因组序列分析[J].病毒学报,2013,29(03):293-298.DOI:10.13242/j.cnki.bingduxuebao.002389.
2013-05-21
2013-05-21
2013-05-21