山东第一医科大学公共卫生与健康管理学院;山东省疾病预防控制中心;
札如病毒(Sapovirus, SaV)具有高度的遗传多样,是引起散发或暴发病毒性胃肠炎的重要病原体之一。为了解中国东部地区人类SaV的流行情况及其基因型多样性,本研究于2020年1月至2022年12月,在山东省济南市采集并处理了36份污水样本,实时荧光定量RT-PCR检测显示33份(91.67%)污水样本为阳性;使用巢式RT-PCR对部分VP1序列进行扩增,电泳显示16份样本为阳性(44.4%);对扩增产物分别进行下一代测序(Next generation sequencing, NGS)和分析,共鉴定出7种SaV基因型,属于GI、GII和GV三个基因群,包括5种主要基因型(GI.1、GI.2、GI.6、GII.1和GV.1)和2种不常见的基因型(GII.3和GII.5);研究期间内,优势基因型在2021年4月前后由GI.2型转换为GI.1型。基于部分VP1序列的系统发生学分析显示本地序列和外来序列在某些分支中聚集,具有密切的亲缘关系。本研究丰富了新冠疫情以来三年内札如病毒的分子流行病学特征,证明了基于NGS的环境污水监测可以增加人们对SaV流行状况的认识,对深入研究我国SaV的分布及流行具有重大意义。
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基本信息:
DOI:10.13242/j.cnki.bingduxuebao.004500
中图分类号:R373
引用信息:
[1]龙森,纪峰,林小娟等.2020-2022年济南市污水中札如病毒的型别构成及其分子特征[J].病毒学报,2024,40(03):528-535.DOI:10.13242/j.cnki.bingduxuebao.004500.
基金信息:
泰山学者青年专家计划项目(项目号:tsqn202103187),题目:基于同一健康理念和下一代测序技术新型病毒的发现、溯源和进化动态研究; 国家自然科学基金面上项目(项目号:81573209),题目:环境污水中人类胃肠炎病毒的型别多样性、遗传进化规律及其与人群疾病发生的关系~~