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2024 03 v.40 528-535
2020-2022年济南市污水中札如病毒的型别构成及其分子特征
基金项目(Foundation): 泰山学者青年专家计划项目(项目号:tsqn202103187),题目:基于同一健康理念和下一代测序技术新型病毒的发现、溯源和进化动态研究;; 国家自然科学基金面上项目(项目号:81573209),题目:环境污水中人类胃肠炎病毒的型别多样性、遗传进化规律及其与人群疾病发生的关系~~
邮箱(Email): zexin.tao@163.com;aqxuepi@163.com;
DOI: 10.13242/j.cnki.bingduxuebao.004500
中文作者单位:

山东第一医科大学公共卫生与健康管理学院;山东省疾病预防控制中心;

摘要(Abstract):

札如病毒(Sapovirus, SaV)具有高度的遗传多样,是引起散发或暴发病毒性胃肠炎的重要病原体之一。为了解中国东部地区人类SaV的流行情况及其基因型多样性,本研究于2020年1月至2022年12月,在山东省济南市采集并处理了36份污水样本,实时荧光定量RT-PCR检测显示33份(91.67%)污水样本为阳性;使用巢式RT-PCR对部分VP1序列进行扩增,电泳显示16份样本为阳性(44.4%);对扩增产物分别进行下一代测序(Next generation sequencing, NGS)和分析,共鉴定出7种SaV基因型,属于GI、GII和GV三个基因群,包括5种主要基因型(GI.1、GI.2、GI.6、GII.1和GV.1)和2种不常见的基因型(GII.3和GII.5);研究期间内,优势基因型在2021年4月前后由GI.2型转换为GI.1型。基于部分VP1序列的系统发生学分析显示本地序列和外来序列在某些分支中聚集,具有密切的亲缘关系。本研究丰富了新冠疫情以来三年内札如病毒的分子流行病学特征,证明了基于NGS的环境污水监测可以增加人们对SaV流行状况的认识,对深入研究我国SaV的分布及流行具有重大意义。

关键词(KeyWords): 人类札如病毒;;环境监测;;下一代测序;;分子流行病学
参考文献 [1] Kumthip K, Khamrin P, Ushijima H, et al. Genetic recombination and diversity of sapovirus in pediatric patients with acute gastroenteritis in Thailand, 2010-2018[J]. PeerJ, 2020, 8:e8520. DOI:10.7717/peerj.8520.
[2] Diez-Valcarce M, Montmayeur A, Tatusov R, et al.Near-complete human sapovirus genome sequences from Kenya[J]. Microbiol Resour Announc, 2019, 8(7):1602-1618. DOI:10.1128/MRA.01602-18.
[3] Sánchez GJ, Mayta H, Pajuelo MJ, et al. Epidemiology of sapovirus infections in a birth cohort in Peru[J]. Clin Infect Dis, 2018, 66(12):1858-1863. DOI:10.1093/cid/cix1103.
[4] Varela MF, Rivadulla E, Lema A, et al. Human sapovirus among outpatients with acute gastroenteritis in Spain:a one-year study[J]. Viruses, 2019, 11(2):144.DOI:10.3390/v11020144.
[5] Liu X, Jahuira H, Gilman RH, et al. Etiological role and repeated infections of sapovirus among children aged less than 2 Years in a cohort study in a peri-urban community of Peru[J]. J Clin Microbiol, 2016, 54(6):1598-1604. DOI:10.1128/JCM.03133-15.
[6] La Rosa G, Iaconelli M, Mancini P, et al. First detection of SARS-CoV-2 in untreated wastewaters in Italy[J]. Sci Total Environ, 2020, 736:139652. DOI:10.1016/j.scitotenv.2020.139652.
[7] Suffredini E, Iaconelli M, Equestre M, et al. Genetic diversity among genogroup II noroviruses and progressive emergence of GII.17 in wastewaters in Italy(2011-2016)revealed by next-generation and sanger sequencing[J]. Food Environ Virol, 2018, 10(2):141-150. DOI:10.1007/s12560-017-9328-y.
[8] Iaconelli M, Valdazo-González B, Equestre M, et al.Molecular characterization of human adenoviruses in urban wastewaters using next generation and Sanger sequencing[J]. Water Res, 2017, 121:240-247. DOI:10.1016/j.watres.2017.05.039.
[9] Mancini P, Bonanno Ferraro G, Iaconelli M, et al.Molecular characterization of human sapovirus in untreated sewage in Italy by amplicon-based Sanger and next-generation sequencing[J]. J Appl Microbiol, 2019,126(1):324-331. DOI:10.1111/jam.14129.
[10]Oka T, Katayama K, Hansman GS, et al. Detection of human sapovirus by real-time reverse transcriptionpolymerase chain reaction[J]. J Med Virol, 2006, 78(10):1347-1353. DOI:10.1002/jmv.20699.
[11]Kitajima M, Oka T, Haramoto E, et al. Detection and genetic analysis of human sapoviruses in river water in Japan[J]. Appl Environ Microbiol, 2010, 76(8):2461-2467. DOI:10.1128/AEM.02739-09..
[12]Oka T, Katayama K, Hansman GS, et al. Detection of human sapovirus by real-time reverse transcriptionpolymerase chain reaction[J]. J Med Virol, 2006, 78(10):1347-1353. DOI:10.1002/jmv.20699.
[13]Fioretti JM, Rocha MS, Fumian TM, et al. Occurrence of human sapoviruses in wastewater and stool samples in Rio De Janeiro, Brazil[J]. J Appl Microbiol, 2016, 121(3):855-862. DOI:10.1111/jam.13205.
[14]Varela MF, Ouardani I, Kato T, et al. Sapovirus in wastewater treatment plants in Tunisia:prevalence,removal, and genetic characterization[J]. Appl Environ Microbiol, 2018, 84(6):e02093-17. DOI:10.1128/AEM.02093-17.
[15]Song K, Lin X, Liu Y, et al. Detection of human sapoviruses in sewage in China by next generation sequencing[J]. Food Environ Virol, 2021, 13(2):270-280. DOI:10.1007/s12560-021-09469-x.
[16]CCDC. 2020年全国法定传染病疫情概况[R].北京:疾病预防控制局.
[17]Geng MJ, Zhang HY, Yu LJ, et al. Changes in notifiable infectious disease incidence in China during the COVID-19 pandemic[J]. Nat Commun, 2021, 12(1):6923. DOI:10.1038/s41467-021-27292-7.
[18]Harada S, Okada M, Yahiro S, et al. Surveillance of pathogens in outpatients with gastroenteritis and characterization of sapovirus strains between 2002 and2007 in Kumamoto Prefecture, Japan[J]. J Med Virol,2009, 81(6):1117-1127. DOI:10.1002/jmv.21454.
[19]Kitajima M, Rachmadi AT, Iker BC, et al. Temporal variations in genotype distribution of human sapoviruses and Aichi virus 1 in wastewater in Southern Arizona,United States[J]. J Appl Microbiol, 2018, 124(5):1324-1332. DOI:10.1111/jam.
[20]Murray TY, Mans J, Taylor MB. Human calicivirus diversity in wastewater in South Africa[J]. J Appl Microbiol, 2013, 114(6):1843-1853. DOI:10.1111/jam.12167.
[21]Okitsu S, Khamrin P, Thongprachum A, et al.Diversity of human sapovirus genotypes detected in Japanese pediatric patients with acute gastroenteritis,2014-2017[J]. J Med Virol, 2021, 93(8):4865-4874.DOI:10.1002/jmv.26934.
[22]郝夏琼,任燕,许少坚,等.深圳市龙华区2019年札如病毒基因分型以及进化分析[J].中国热带医学,21(3):247-250. DOI:10.13604/j.cnki.46-1064/r.2021.03.11
[23]金晶,史永林,张文艳,等.一起急性胃肠炎暴发疫情的病原分子特征分析[J].安徽预防医学杂志,27(2):109-111. DOI:10.19837f.cnki.ahyf.2021.02.006.
[24]姚萍,李琼,蒋霞,等.常州市2019-2022年学校札如病毒聚集性疫情的流行病学及病毒基因特征分析[J].中国学校卫生,44(10):1574-1577. DOI:10.16835/j.cnki.1000-9817.2023.10.030.
[25]Sano D, Pérez-Sautu U, Guix S, et al. Quantification and genotyping of human sapoviruses in the Llobregat river catchment, Spain[J]. Appl Environ Microbiol,2011, 77(3):1111-1114. DOI:10.1128/AEM. 01721-10.
[26]Ibrahim C, Hammami S, Chérif N, et al. Detection of sapoviruses in two biological lines of Tunisian hospital wastewater treatment[J]. Int J Environ Health Res,2019, 29(4):400-413. DOI:10.1080/09603123.2018.1546835.
[27]Le TH, Ng C, Chen H, et al. Occurrences and characterization of antibiotic-resistant bacteria and genetic determinants of hospital wastewater in a tropical country[J]. Antimicrob Agents Chemother, 2016, 60(12):7449-7456. DOI:10.1128/AAC.01556-16.
[28]Tao Z, Xu M, Lin X, et al. Environmental surveillance of genogroup I and II noroviruses in Shandong Province,China in 2013[J]. Sci Rep, 2015, 5:17444. DOI:10.1038/srep17444.

基本信息:

DOI:10.13242/j.cnki.bingduxuebao.004500

中图分类号:R373

引用信息:

[1]龙森,纪峰,林小娟等.2020-2022年济南市污水中札如病毒的型别构成及其分子特征[J].病毒学报,2024,40(03):528-535.DOI:10.13242/j.cnki.bingduxuebao.004500.

基金信息:

泰山学者青年专家计划项目(项目号:tsqn202103187),题目:基于同一健康理念和下一代测序技术新型病毒的发现、溯源和进化动态研究;; 国家自然科学基金面上项目(项目号:81573209),题目:环境污水中人类胃肠炎病毒的型别多样性、遗传进化规律及其与人群疾病发生的关系~~

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