| 4,409 | 47 | 373 |
| 下载次数 | 被引频次 | 阅读次数 |
截至2021年5月11日,基于Pango命名法,依据新型冠状病毒(SARS-CoV-2)的基因组变异变迁将其划分为1 281种亚型或分支。而SARS-CoV-2刺突(spike,S)蛋白的变异直接影响病毒的生物学功能。2020年下半年至今,全球多个国家和地区监测发现SARS-CoV-2的S蛋白发生氨基酸突变,特别是受体结合区或单克隆抗体结合位点氨基酸突变引起病毒的传播力和致病力改变以及部分免疫逃逸等。世界卫生组织将重要变异株划分为"关切变异株(variant of concern,VOC)"和"关注变异株(variant of interest,VOI)"。其中,VOC有4个,分别是VOC202012/01、501Y.V2、P.1和B.1.617;VOI有6个,分别是CAL.20C、P.2、B.1.526、B.1.525、B.1.616和P.3。一些氨基酸突变在多个VOC和VOI病毒株中交叉出现或同时出现,E484K/Q等重要氨基酸突变引起的部分免疫逃逸导致全球现有疫苗免疫效力下降,但现有新冠疫苗对VOC和VOI变异株仍然有效。本文通过对SARS-CoV-2变异株流行概况及S蛋白重要氨基酸突变特征进行归纳分析,为新冠病毒变异株的监测、防控和二代疫苗的研制策略提供科学参考。
Abstract：As of 11 thMay 2021,SARS-CoV-2 has been divided into 1281 subtypes or branches by the Pango nomenclature system. The mutation of spike(S)protein of SARS-CoV-2 directly affects the biological function of the virus. Since the second half of 2020,many countries or regions have found that S protein of SARS-CoV-2 occurred mutations,especially the mutations in the receptor binding domain or monoclonal antibody binding sites leading to the transmissibility and pathogenicity changes and partial immune escape. The World Health Organization categorized the key variants into"variants of concern(VOCs)"and"variants of interest(VOIs)".There are four VOCs,called VOC 202012/01,501 Y.V2,P.1,and B.1.617;and six VOIs,called CAL.20 C,P.2,B.1.526,B.1.525,B.1.616,and P.3. Some amino acid mutations are shared or shifted by multiple VOCs and VOIs. Partial immune escape caused by E484 K/Q or other key amino acid mutations has reduced the immunity effectiveness of the existing vaccines worldwide,but the existing COVID-19 vaccines are still effective against current VOCs and VOIs. In this review,we summarize the epidemiological situation of the SARS-CoV-2 variants and the characteristics of key amino acid mutations in the S protein,to provide a scientific reference for SARS-CoV-2 variants surveillance,prevention and control,and development strategy of second-generation vaccines.
[1]Duchene S,Featherstone L,Haritopoulou-Sinanidou M,Rambaut A,Lemey P,Baele G.Temporal signal and the phylodynamic threshold of SARS-CoV-2[J/OL].Virus Evol,2020,6(2):veaa061.
[2]Smith E C,Blanc H,Surdel M C,Vignuzzi M,Denison M R.Coronaviruses lacking exoribonuclease activity are susceptible to lethal mutagenesis:evidence for proofreading and potential therapeutics[J/OL].PLoS Pathog,2013,9(8):e1003565.
[3]Zhou B,Thi Nhu Thao T,Hoffmann D,Taddeo A,Ebert N,Labroussaa F,Pohlmann A,King J,Steiner S,Kelly J N,Portmann J,Halwe N J,Ulrich L,Trueb B S,Fan X,Hoffmann B,Wang L,Thomann L,Lin X,Stalder H,Pozzi B,de Brot S,Jiang N,Cui D,Hossain J,Wilson M,Keller M,Stark T J,Barnes JR,Dijkman R,Jores J,Benarafa C,Wentworth D E,Thiel V,Beer M.SARS-CoV-2 spike D614G change enhances replication and transmission[J].Nature,2021,592(7852):122-127.
[4]Wang Z,Schmidt F,Weisblum Y,Muecksch F,Barnes C O,Finkin S,Schaefer-Babajew D,Cipolla M,Gaebler C,Lieberman J A,Oliveira T Y,Yang Z,Abernathy M E,Huey-Tubman K E,Hurley A,Turroja M,West K A,Gordon K,Millard K G,Ramos V,Da Silva J,Xu J,Colbert R A,Patel R,Dizon J,Unson-O'Brien C,Shimeliovich I,Gazumyan A,Caskey M,Bjorkman P J,Casellas R,Hatziioannou T,Bieniasz P D,Nussenzweig M C.mRNA vaccineelicited antibodies to SARS-CoV-2 and circulating variants[J].Nature,2021,592(7855):616-622.
[5]GISAID.Timecourse of clade distribution in collected sequences[DB/OL].2021.Available from:https：//www.gisaid.org/.
[6]World Health Organization.SARS-CoV-2 minkassociated variant strain-Denmark[OL].2020.Available from:https：//www.who.int/csr/don/06-november-2020-mink-associated-sars-cov2-denmark/en/.
[7]World Health Organization.Weekly epidemiological update-25 February 2021[OL].2021.Available from:https：//www.who.int/publications/m/item/covid-19-weekly-epidemiological-update.
[8]冯晔囡,陈志肖,梦遥,赵翔,宋洋,王佶,许文波,陈操,王世文.新型冠状病毒变异株VOC 202012/01的全球早期传播与刺突蛋白进化特征分析[J].病毒学报,2021,37(02):267-273.
[9]World Health Organization.Weekly epidemiological update on COVID-19-11 May 2021[OL].2021.Available from:https：//www.who.int/publications/m/item/weekly-epidemiological-update-on-covid-19-11-may-2021.
[10]England P H.Investigation of SARS-CoV-2 variants of concern-England.Technical briefing 6[OL].2021.Available from:https：//www.gov.uk/government/publications/investigation-of-novel-sars-cov-2-variantvariant-of-concern-20201201.
[11]Davies N G,Abbott S,Barnard R C,Jarvis C I,Kucharski A J,Munday J D,Pearson C A B,Russell TW,Tully D C,Washburne A D,Wenseleers T,Gimma A,Waites W,Wong K L M,van Zandvoort K,Silverman J D,Group C C-W,Consortium C-G U,Diaz-Ordaz K,Keogh R,Eggo R M,Funk S,Jit M,Atkins K E,Edmunds W J.Estimated transmissibility and impact of SARS-CoV-2 lineage B.1.1.7 in England[J/OL].Science,2021,372(6538):eabg3055
[12]Frampton D,Rampling T,Cross A,Bailey H,Heaney J,Byott M,Scott R,Sconza R,Price J,Margaritis M,Bergstrom M,Spyer M J,Miralhes P B,Grant P,Kirk S,Valerio C,Mangera Z,Prabhahar T,MorenoCuesta J,Arulkumaran N,Singer M,Shin G Y,Sanchez E,Paraskevopoulou S M,Pillay D,McKendry R A,Mirfenderesky M,Houlihan C F,Nastouli E.Genomic characteristics and clinical effect of the emergent SARS-CoV-2 B.1.1.7 lineage in London,UK:a whole-genome sequencing and hospital-based cohort study[J/OL].Lancet Infect Dis,2021.DOI:10.1016/S1473-3099(21)00170-5.
[13]Golubchik T,Lythgoe K A,Hall M,Ferretti L,Fryer H R,MacIntyre-Cockett G,de Cesare M,Trebes A,Piazza P,Buck D,Todd J A,Fraser C,Bonsall D.Early analysis of a potential link between viral load and the N501Y mutation in the SARS-COV-2 spike protein[OL].medRxiv,2021.DOI:10.1101/2021.01.12.20249080.
[14]England P H.NERVTAG update note on B.1.1.7severity[OL].2021.Available from:https：//assets.publishing.service.gov.uk/government/uploads/system/uploads/attachment_data/file/961042/S1095_NERVTAG_update_note_on_B.1.1.7_severity_20210211.pdf.
[15]Challen R,Brooks-Pollock E,Read J M,Dyson L,Tsaneva-Atanasova K,Danon L.Risk of mortality in patients infected with SARS-CoV-2 variant of concern202012/1:matched cohort study[J/OL].BMJ,2021,372:n579.
[16]Davies N G,Jarvis C I,Group C C-W,Edmunds W J,Jewell N P,Diaz-Ordaz K,Keogh R H.Increased mortality in community-tested cases of SARS-CoV-2lineage B.1.1.7[J/OL].Nature,2021.
[17]Wang P,Nair M S,Liu L,Iketani S,Luo Y,Guo Y,Wang M,Yu J,Zhang B,Kwong P D,Graham B S,Mascola J R,Chang J Y,Yin M T,Sobieszczyk M,Kyratsous C A,Shapiro L,Sheng Z,Huang Y,Ho DD.Antibody Resistance of SARS-CoV-2 Variants B.1.351 and B.1.1.7[J/OL].Nature,2021.DOI:10.1038/s41586-021-03426-1.
[18]Collier D A,De Marco A,Ferreira I,Meng B,Datir R,Walls A C,Kemp S S,Bassi J,Pinto D,Fregni CS,Bianchi S,Tortorici M A,Bowen J,Culap K,Jaconi S,Cameroni E,Snell G,Pizzuto M S,Pellanda A F,Garzoni C,Riva A,Collaboration C-N B C-,Elmer A,Kingston N,Graves B,McCoy L E,Smith K G C,Bradley J R,Temperton N,Lourdes CeronGutierrez L,Barcenas-Morales G,consortium C-G U,Harvey W,Virgin H W,Lanzavecchia A,Piccoli L,Doffinger R,Wills M,Veesler D,Corti D,Gupta R K.Sensitivity of SARS-CoV-2 B.1.1.7 to mRNA vaccineelicited antibodies[J/OL].Nature,2021.DOI:10.1038/s41586-021-03412-7.
[19]Garcia-Beltran W F,Lam E C,St Denis K,Nitido AD,Garcia Z H,Hauser B M,Feldman J,Pavlovic MN,Gregory D J,Poznansky M C,Sigal A,Schmidt AG,Iafrate A J,Naranbhai V,Balazs A B.Multiple SARS-CoV-2 variants escape neutralization by vaccineinduced humoral immunity[J/OL].Cell,2021.DOI:10.1016/j.cell.2021.03.013.
[20]Liu Y,Liu J,Xia H,Zhang X,Fontes-Garfias C R,Swanson K A,Cai H,Sarkar R,Chen W,Cutler M,Cooper D,Weaver S C,Muik A,Sahin U,Jansen KU,Xie X,Dormitzer P R,Shi P Y.Neutralizing Activity of BNT162b2-Elicited Serum[J/OL].N Engl JMed,2021.DOI:10.1056/NEJMc2102017.
[21]Planas D,Bruel T,Grzelak L,Guivel-Benhassine F,Staropoli I,Porrot F,Planchais C,Buchrieser J,Rajah M M,Bishop E,Albert M,Donati F,Prot M,Behillil S,Enouf V,Maquart M,Smati-Lafarge M,Varon E,Schortgen F,Yahyaoui L,Gonzalez M,De Seze J,Pere H,Veyer D,Seve A,Simon-Loriere E,FafiKremer S,Stefic K,Mouquet H,Hocqueloux L,van der Werf S,Prazuck T,Schwartz O.Sensitivity of infectious SARS-CoV-2 B.1.1.7 and B.1.351 variants to neutralizing antibodies[J/OL].Nat Med,2021.DOI:10.1038/s41591-021-01318-5.
[22]Bernal J L,Andrews N,Gower C,Stowe J,Robertson C,Tessier E,Simmons R,Cottrell S,Roberts R,O’Doherty M,Brown K,Cameron C,Stockton D,McMenamin J,Ramsay M.Early effectiveness of COVID-19 vaccination with BNT162b2 mRNA vaccine and ChAdOx1 adenovirus vector vaccine on symptomatic disease,hospitalisations and mortality in older adults in England[OL].medRxiv,2021.DOI:10.1101/2021.03.01.21252652.
[23]Lumley S F,Rodger G,Constantinides B,Sanderson N,Chau K K,Street T L,O’Donnell D,Howarth A,Hatch S B,Marsden B D,Cox S,James T,Warren F,Peck L J,Ritter T G,de Toledo Z,Warren L,Axten D,Cornall R J,Jones E Y,Stuart D I,Screaton G,Ebner D,Hoosdally S,Chand M,,Crook D W,O’Donnell A-M,Conlon C P,Pouwels K B,Walker A S,Peto T E,Hopkins S,Walker T M,Stoesser N E,Matthews P C,Jeffery K,Eyre D W.An observational cohort study on the incidence of SARS-CoV-2 infection and B.1.1.7 variant infection in healthcare workers by antibody and vaccination status[OL].medRxiv,2021.DOI:10.1101/2021.03.09.21253218.
[24]Shen X,Tang H,McDanal C,Wagh K,Fischer W,Theiler J,Yoon H,Li D,Haynes B F,Sanders K O,Gnanakaran S,Hengartner N,Pajon R,Smith G,Glenn G M,Korber B,Montefiori D C.SARS-CoV-2variant B.1.1.7 is susceptible to neutralizing antibodies elicited by ancestral spike vaccines[J].Cell Host Microbe,2021,29(4):529-539.
[25]Mahase E.Covid-19:Novavax vaccine efficacy is 86%against UK variant and 60%against South African variant[J/OL].BMJ,2021,372:n296.
[26]Wang G L,Wang Z Y,Duan L J,Meng Q C,Jiang MD,Cao J,Yao L,Zhu K L,Cao W C,Ma M J.Susceptibility of Circulating SARS-CoV-2 Variants to Neutralization[J/OL].N Engl J Med,2021.DOI:10.1056/NEJMc2103022.
[27]Emary K R W,Golubchik T,Aley P K,Ariani C V,Angus B,Bibi S,Blane B,Bonsall D,Cicconi P,Charlton S,Clutterbuck E A,Collins A M,Cox T,Darton T C,Dold C,Douglas A D,Duncan C J A,Ewer K J,Flaxman A L,Faust S N,Ferreira D M,Feng S,Finn A,Folegatti P M,Fuskova M,Galiza E,Goodman A L,Green C M,Green C A,Greenland M,Hallis B,Heath P T,Hay J,Hill H C,Jenkin D,Kerridge S,Lazarus R,Libri V,Lillie P J,Ludden C,Marchevsky N G,Minassian A M,McGregor A C,Mujadidi Y F,Phillips D J,Plested E,Pollock K M,Robinson H,Smith A,Song R,Snape M D,Sutherland R K,Thomson E C,Toshner M,Turner DP J,Vekemans J,Villafana T L,Williams C J,Hill AV S,Lambe T,Gilbert S C,Voysey M,Ramasamy MN,Pollard A J,consortium C-G U,Project A,Oxford C-V T G.Efficacy of ChAdOx1 nCoV-19(AZD1222)vaccine against SARS-CoV-2 variant of concern 202012/01(B.1.1.7):an exploratory analysis of a randomised controlled trial[J].Lancet,2021,397(10282):1351-1362.
[28]Tegally H,Wilkinson E,Giovanetti M,Iranzadeh A,Fonseca V,Giandhari J,Doolabh D,Pillay S,San EJ,Msomi N,Mlisana K,von Gottberg A,Walaza S,Allam M,Ismail A,Mohale T,Glass A J,Engelbrecht S,Van Zyl G,Preiser W,Petruccione F,Sigal A,Hardie D,Marais G,Hsiao M,Korsman S,Davies MA,Tyers L,Mudau I,York D,Maslo C,Goedhals D,Abrahams S,Laguda-Akingba O,Alisoltani-Dehkordi A,Godzik A,Wibmer C K,Sewell B T,Lourenco J,Alcantara L C J,Kosakovsky Pond S L,Weaver S,Martin D,Lessells R J,Bhiman J N,Williamson C,de Oliveira T.Emergence of a SARS-CoV-2 variant of concern with mutations in spike glycoprotein[J].Nature,2021.DOI:10.1038/s41586-021-03402-9.
[29]Carl A P,Nicholas D,Adam J K,CMMID COVID-19working group,Rosalind M E.Estimates of severity and transmissibility of novel SARS-CoV-2 variant 501Y.V2in South Africa[OL].2021.Available from:https：//cmmid.github.io/topics/covid19/sa-novel-variant.html.
[30]Wibmer C K,Ayres F,Hermanus T,Madzivhandila M,Kgagudi P,Oosthuysen B,Lambson B E,de Oliveira T,Vermeulen M,van der Berg K,Rossouw T,Boswell M,Ueckermann V,Meiring S,von Gottberg A,Cohen C,Morris L,Bhiman J N,Moore P L.SARS-CoV-2 501Y.V2 escapes neutralization by South African COVID-19 donor plasma[J/OL].Nat Med,2021.DOI:10.1038/s41591-021-01285-x.
[31]Shinde V,Bhikha S,Hoosain Z,Archary M,Bhorat Q,Fairlie L,Lalloo U,Masilela M S L,Moodley D,Hanley S,Fouche L,Louw C,Tameris M,Singh N,Goga A,Dheda K,Grobbelaar C,Kruger G,CarrimGaney N,Baillie V,de Oliveira T,Koen A L,Lombaard J J,Mngqibisa R,Bhorat A a E,BenadéG,Lalloo N,Pitsi A,Vollgraaff P-L,Luabeya A,Esmail A,Petrick F G,Jose A O,Foulkes S,Ahmed K,Thombrayil A,Fries L,Cloney-Clark S,Zhu M,Bennett C,Albert G,Faust E,Plested J S,Robertson A,Neal S,Cho I,Glenn G M,Dubovsky F,Madhi SA,.Preliminary Efficacy of the NVX-CoV2373 Covid-19 Vaccine Against the B.1.351 Variant[OL].medRxiv,2021.DOI:10.1101/2021.02.25.21252477.
[32]Wu K,Werner A P,Koch M,Choi A,Narayanan E,Stewart-Jones G B E,Colpitts T,Bennett H,BoyogluBarnum S,Shi W,Moliva J I,Sullivan N J,Graham BS,Carfi A,Corbett K S,Seder R A,Edwards D K.Serum Neutralizing Activity Elicited by mRNA-1273Vaccine[J/OL].N Engl J Med,2021.DOI:10.1056/NEJMc2102179.
[33]Shen X,Tang H,Pajon R,Smith G,Glenn G M,Shi W,Korber B,Montefiori D C.Neutralization of SARS-CoV-2 Variants B.1.429 and B.1.351[J/OL].N Engl JMed,2021.DOI:10.1056/NEJMc2103740.
[34]Edara V V,Norwood C,Floyd K,Lai L,DavisGardner M E,Hudson W H,Mantus G,Nyhoff L E,Adelman M W,Fineman R,Patel S,Byram R,Gomes D N,Michael G,Abdullahi H,Beydoun N,Panganiban B,McNair N,Hellmeister K,Pitts J,Winters J,Kleinhenz J,Usher J,O’Keefe J B,Piantadosi A,Waggoner J J,Babiker A,Stephens D S,Anderson E J,Edupuganti S,Rouphael N,Ahmed R,Wrammert J,Suthar M S.Infection and vaccine-induced antibody binding and neutralization of the B.1.351 SARS-CoV-2 variant[J].Cell Host&Microbe,2021,29(4):516-521.
[35]Madhi S A,Baillie V,Cutland C L,Voysey M,Koen A L,Fairlie L,Padayachee S D,Dheda K,Barnabas SL,Bhorat Q E,Briner C,Kwatra G,Ahmed K,Aley P,Bhikha S,Bhiman J N,Bhorat A E,du Plessis J,Esmail A,Groenewald M,Horne E,Hwa S H,Jose A,Lambe T,Laubscher M,Malahleha M,Masenya M,Masilela M,McKenzie S,Molapo K,Moultrie A,Oelofse S,Patel F,Pillay S,Rhead S,Rodel H,Rossouw L,Taoushanis C,Tegally H,Thombrayil A,van Eck S,Wibmer C K,Durham N M,Kelly E J,Villafana T L,Gilbert S,Pollard A J,de Oliveira T,Moore P L,Sigal A,Izu A,Group N-S G W-V C.Efficacy of the ChAdOx1 nCoV-19 Covid-19 Vaccine against the B.1.351 Variant[J/OL].N Engl J Med,2021.DOI:10.1056/NEJMoa2102214.
[36]Huang B,Dai L,Wang H,Hu Z,Yang X,Tan W,Gao G F.Neutralization of SARS-CoV-2 VOC 501Y.V2 by human antisera elicited by both inactivated BBIBP-CorV and recombinant dimeric RBD ZF2001 vaccines[OL].bioRxiv,2021.DOI:10.1101/2021.02.01.429069.
[37]Moyo-Gwete T,Madzivhandila M,Makhado Z,Ayres F,Mhlanga D,Oosthuysen B,Lambson B E,Kgagudi P,Tegally H,Iranzadeh A,Doolabh D,Tyers L,Chinhoyi L R,Mennen M,Skelm S,Wibmer C K,Bhiman J N,Ueckermann V,Rossouw T,Boswell M,de Oliveira T,Williamson C,Burgers W A,Ntusi N,Morris L,Moore P L.SARS-CoV-2 501Y.V2(B.1.351) elicits cross-reactive neutralizing antibodies[OL].bioRxiv,2021.DOI:10.1101/2021.03.06.434193.
[38]Moderna.Moderna Announces it has Shipped VariantSpecific Vaccine Candidate,mRNA-1273.351,to NIHfor Clinical Study[OL].2021.Available from:https：//investors.modernatx.com/news-releases/news-releasedetails/moderna-announces-it-has-shipped-variantspecific-vaccine.
[39]Faria N R,Mellan T A,Whittaker C,Claro I M,Candido D D S,Mishra S,Crispim M A E,Sales F CS,Hawryluk I,McCrone J T,Hulswit R J G,Franco L A M,Ramundo M S,de Jesus J G,Andrade P S,Coletti T M,Ferreira G M,Silva C A M,Manuli E R,Pereira R H M,Peixoto P S,Kraemer M U G,Gaburo N,Jr.,Camilo C D C,Hoeltgebaum H,Souza W M,Rocha E C,de Souza L M,de Pinho M C,Araujo L JT,Malta F S V,de Lima A B,Silva J D P,Zauli D AG,Ferreira A C S,Schnekenberg R P,Laydon D J,Walker P G T,Schluter H M,Dos Santos A L P,Vidal M S,Del Caro V S,Filho R M F,Dos Santos HM,Aguiar R S,Proenca-Modena J L,Nelson B,Hay JA,Monod M,Miscouridou X,Coupland H,Sonabend R,Vollmer M,Gandy A,Prete C A,Jr.,Nascimento V H,Suchard M A,Bowden T A,Pond S L K,Wu CH,Ratmann O,Ferguson N M,Dye C,Loman N J,Lemey P,Rambaut A,Fraiji N A,Carvalho M,Pybus O G,Flaxman S,Bhatt S,Sabino E C.Genomics and epidemiology of the P.1 SARS-CoV-2 lineage in Manaus,Brazil[J/OL].Science,2021.DOI:10.1126/science.abh2644.
[40]HoffmannM,Arora,P.,Gro?,R.,Seidel,A.,H?rnich,B.F.,Hahn,A.S.,Krüger,N.,Graichen,L.,Hofmann-Winkler,H.,Kempf,A.,Winkler,M.S.,Schulz,S.,J?ck,H.-M.,Jahrsd?rfer,B.,Schrezenmeier,H.,Müller,M.,Kleger,A.,Münch,J.,P?hlmann,S.,SA.SARS-CoV-2 variants B.1.351and P.1escape from neutralizing antibodies.[J/OL].Cell,2021.DOI:10.1016/j.cell.2021.03.036.
[41]Wang P,Casner R G,Nair M S,Wang M,Yu J,Cerutti G,Liu L,Kwong P D,Huang Y,Shapiro L,Ho D D.Increased resistance of SARS-CoV-2 variant P.1 to antibody neutralization[J/OL].Cell Host Microbe,2021.DOI:10.1016/j.chom.2021.04.007.
[42]DeSouza W M,Mariene R.Sesti C R,Coimbra L D,Toledo T D,Parise P L,Barbosa P P,Bispo S K,Mofatto L S,Simeoni C L,Brunetti N S,Claro I M,Duarte A S,Coletti T M,Zangirolami A B,Costa LC,Gomes A B,Buscaratti L I,Sales F C,Costa V A,Franco L A,Candido D S,Pybus O G,de Jesus J G,Silva C A,Ramundo M S,Ferreira G M,Pinho M C,Souza L M,Rocha E C,Andrade P S,Crispim M A,Maktura G C,Manuli E R,Santos M N,Camilo C C,Angerami R N,Moretti M L,Spilki F R,Arns C W,Addas C M,Benites B D,Mori M A,Gaburo N,Dye C,Wu C H,Marques S H,Marques R E,Farias A,Diamond M S,Faria N R,Sabino E C,Granja F,Proenca J L.Levels of SARS-CoV-2 Lineage P.1Neutralization by Antibodies Elicited after Natural Infection and Vaccination[OL].SSRN,2021.DOI:10.2139/ssrn.3793486.
[43]Nonaka C K V,Franco M M,Graf T,de Lorenzo Barcia C A,de Avila Mendonca R N,de Sousa K A F,Neiva L M C,Fosenca V,Mendes A V A,de Aguiar R S,Giovanetti M,de Freitas Souza B S.Genomic Evidence of SARS-CoV-2 Reinfection Involving E484KSpike Mutation,Brazil[J].Emerg Infect Dis,2021,27(5):1522-1524.
[44]Resende P C,Bezerra J F,Teixeira Vasconcelos R H,Arantes I,Appolinario L,Mendonca A C,Paixao A C,Duarte A C,Silva T,Rocha A S,Lima A B M,Pauvolid-Correa A,Motta F C,Teixeira D L F,de Oliveira Carneiro T F,Neto F P F,Herbster I D,Leite A B,Riediger I N,do Carmo Debur M,Naveca F G,Almeida W,Livorati M,Bello G,Siqueira M M.Severe Acute Respiratory Syndrome Coronavirus 2 P.2Lineage Associated with Reinfection Case,Brazil,JuneOctober 2020[J/OL].Emerg Infect Dis,2021,27(7).DOI:10.3201/eid2707.210401.
[45]Deng X,Garcia-Knight M A,Khalid M M,Servellita V,Wang C,Morris M K,Sotomayor-González A,Glasner D R,Reyes K R,Gliwa A S,Reddy N P,Sanchez San Martin C,Federman S,Cheng J,Balcerek J,Taylor J,Streithorst J A,Miller S,Kumar G R,Sreekumar B,Chen P-Y,Schulze-Gahmen U,Taha TY,Hayashi J,Simoneau C R,McMahon S,Lidsky PV,Xiao Y,Hemarajata P,Green N M,Espinosa A,Kath C,Haw M,Bell J,Hacker J K,Hanson C,Wadford D A,Anaya C,Ferguson D,Lareau L F,Frankino P A,Shivram H,Wyman S K,Ott M,Andino R,Chiu C Y.Transmission,infectivity,and antibody neutralization of an emerging SARS-CoV-2variant in California carrying a L452R spike protein mutation[OL].medRxiv,2021.DOI:10.1101/2021.03.07.21252647.
[46]Zhou H,Dcosta B M,Samanovic M I,Mulligan M J,Landau N R,Tada T.B.1.526 SARS-CoV-2 variants identified in New York City are neutralized by vaccineelicited and therapeutic monoclonal antibodies[OL].bioRxiv,2021.DOI:10.1101/2021.03.24.436620.
[47]Zhang W,Davis B D,Chen S S,Sincuir Martinez J M,Plummer J T,Vail E.Emergence of a Novel SARS-CoV-2 Variant in Southern California[J].JAMA,2021,325(13):1324-1326.
[48]Voloch C M,da Silva Francisco R,Jr.,de Almeida LG P,Cardoso C C,Brustolini O J,Gerber A L,Guimaraes A P C,Mariani D,da Costa R M,Ferreira O C,Jr.,Covid19-Ufrj Workgroup L W A C C,Frauches T S,de Mello C M B,Leitao I C,Galliez RM,Faffe D S,Castineiras T,Tanuri A,de Vasconcelos A T R.Genomic characterization of a novel SARS-CoV-2 lineage from Rio de Janeiro,Brazil[J/OL].J Virol,2021.DOI:10.1128/JVI.00119-21.
[49]Annavajhala M K,Mohri H,Zucker J E,Sheng Z,Wang P,Gomez-Simmonds A,Ho D D,Uhlemann A-C.A Novel SARS-CoV-2 Variant of Concern,B.1.526,Identified in New York[OL].medRxiv,2021.DOI:10.1101/2021.02.23.21252259.
[50]Alaa M,Alkuzweny M,Tsueng G,Cano M,Haag E,Zhou J,Zeller M,Matteson N,Wu C,Andersen K G,Su A I,Gangavarapu K,Hughes L D,and the Center for Viral Systems Biology.B.1.525 Lineage Report[DB/OL].Outbreak.info,2021.Available from:https：//outbreak.info/situation-reports.pango=B.1.525.
[51]Bascos N A D,Mirano-Bascos D,Saloma C P.Structural Analysis of Spike Protein Mutations in the SARS-CoV-2 P.3 Variant[OL].bioRxiv,2021.DOI:10.1101/2021.03.06.434059.
[52]Yao H,Song Y,Chen Y,Wu N,Xu J,Sun C,Zhang J,Weng T,Zhang Z,Wu Z,Cheng L,Shi D,Lu X,Lei J,Crispin M,Shi Y,Li L,Li S.Molecular Architecture of the SARS-CoV-2 Virus[J].Cell,2020,183(3):730-738 e713.
[53]Guruprasad L.Human SARS CoV-2 spike protein mutations[J].Proteins,2021,89(5):569-576.
[54]Focosi D,Maggi F.Neutralising antibody escape of SARS-CoV-2 spike protein:Risk assessment for antibody-based Covid-19 therapeutics and vaccines[J/OL].Rev Med Virol,2021.DOI:10.1002/rmv.2231.
[55]Starr T N,Greaney A J,Hilton S K,Ellis D,Crawford K H D,Dingens A S,Navarro M J,Bowen JE,Tortorici M A,Walls A C,King N P,Veesler D,Bloom J D.Deep Mutational Scanning of SARS-CoV-2Receptor Binding Domain Reveals Constraints on Folding and ACE2 Binding[J].Cell,2020,182(5):1295-1310 e1220.
[56]Gu H,Chen Q,Yang G,He L,Fan H,Deng Y Q,Wang Y,Teng Y,Zhao Z,Cui Y,Li Y,Li X F,Li J,Zhang N N,Yang X,Chen S,Guo Y,Zhao G,Wang X,Luo D Y,Wang H,Yang X,Li Y,Han G,He Y,Zhou X,Geng S,Sheng X,Jiang S,Sun S,Qin C F,Zhou Y.Adaptation of SARS-CoV-2 in BALB/c mice for testing vaccine efficacy[J].Science,2020,369(6511):1603-1607.
[57]Rees-Spear C,Muir L,Griffith S A,Heaney J,Aldon Y,Snitselaar J L,Thomas P,Graham C,Seow J,Lee N,Rosa A,Roustan C,Houlihan C F,Sanders R W,Gupta R K,Cherepanov P,Stauss H J,Nastouli E,Investigators S,Doores K J,van Gils M J,McCoy LE.The effect of spike mutations on SARS-CoV-2neutralization[J].Cell Rep,2021,34(12):108890.
[58]Kemp S,Collier D,Datir R,Ferreira I,Gayed S,Jahun A,Hosmillo M,Rees-Spear C,Mlcochova P,Lumb I U,Roberts D J,Chandra A,Temperton N,Sharrocks K,Blane E,Briggs J,van Gils M,Smith K,Bradley J,Smith C,Doffinger R,Ceron-Gutierrez L,Barcenas-Morales G,Pollock D,Goldstein R,Smielewska A,Skittrall J,Gouliouris T,Goodfellow I,Gkrania-Klotsas E,Illingworth C,McCoy L,Gupta R.Neutralising antibodies in Spike mediated SARS-CoV-2 adaptation[OL].medRxiv,2020.DOI:10.1101/2020.12.05.20241927.
[59]Shi P Y,Xie X,Zou J,Fontes-Garfias C,Xia H,Swanson K,Cutler M,Cooper D,Menachery V,Weaver S,Dormitzer P.Neutralization of N501Ymutant SARS-CoV-2 by BNT162b2 vaccine-elicited sera[J/OL].Res Sq,2021.DOI:10.21203/rs.3.rs-143532/v1.
[60]Xie X,Liu Y,Liu J,Zhang X,Zou J,Fontes-Garfias C R,Xia H,Swanson K A,Cutler M,Cooper D,Menachery V D,Weaver S C,Dormitzer P R,Shi P Y.Neutralization of SARS-CoV-2 spike 69/70 deletion,E484K and N501Y variants by BNT162b2 vaccineelicited sera[J].Nat Med,2021,27(4):620-621.
[61]Weisblum Y,Schmidt F,Zhang F,DaSilva J,Poston D,Lorenzi J C,Muecksch F,Rutkowska M,Hoffmann H H,Michailidis E,Gaebler C,Agudelo M,Cho A,Wang Z,Gazumyan A,Cipolla M,Luchsinger L,Hillyer C D,Caskey M,Robbiani D F,Rice C M,Nussenzweig M C,Hatziioannou T,Bieniasz P D.Escape from neutralizing antibodies by SARS-CoV-2spike protein variants[J/OL].Elife,2020.DOI:10.7554/eLife.61312.
[62]Baum A,Fulton B O,Wloga E,Copin R,Pascal K E,Russo V,Giordano S,Lanza K,Negron N,Ni M,Wei Y,Atwal G S,Murphy A J,Stahl N,Yancopoulos G D,Kyratsous C A.Antibody cocktail to SARS-CoV-2 spike protein prevents rapid mutational escape seen with individual antibodies[J].Science,2020,369(6506):1014-1018.
[63]Liu Z,VanBlargan L A,Bloyet L M,Rothlauf P W,Chen R E,Stumpf S,Zhao H,Errico J M,Theel E S,Liebeskind M J,Alford B,Buchser W J,Ellebedy AH,Fremont D H,Diamond M S,Whelan S P J.Identification of SARS-CoV-2 spike mutations that attenuate monoclonal and serum antibody neutralization[J].Cell Host Microbe,2021,29(3):477-488.
[64]Andreano E,Piccini G,Licastro D,Casalino L,Johnson N V,Paciello I,Monego S D,Pantano E,Manganaro N,Manenti A,Manna R,Casa E,Hyseni I,Benincasa L,Montomoli E,Amaro R E,McLellan JS,Rappuoli R.SARS-CoV-2 escape in vitro from a highly neutralizing COVID-19 convalescent plasma[OL].bioRxiv,2020.DOI:10.1101/2020.12.28.424451.
[65]Luan B,Huynh T.Insights on SARS-CoV-2’s Mutations for Evading Human Antibodies:Sacrifice and Survival[OL].bioRxiv,2021.DOI:10.1021/acs.jmedchem.1c00311.
[66]Li Q,Nie J,Wu J,Zhang L,Ding R,Wang H,Zhang Y,Li T,Liu S,Zhang M,Zhao C,Liu H,Nie L,Qin H,Wang M,Lu Q,Li X,Liu J,Liang H,Shi Y,Shen Y,Xie L,Zhang L,Qu X,Xu W,Huang W,Wang Y.SARS-CoV-2 501Y.V2 variants lack higher infectivity but do have immune escape[J].Cell,2021,184(9):2362-2371.e9.
[67]Liu Z,VanBlargan L A,Rothlauf P W,Bloyet L-M,Chen R E,Stumpf S,Zhao H,Errico J M,Theel E S,Ellebedy A H,Fremont D H,Diamond M S,Whelan SP J.Landscape analysis of escape variants identifies SARS-CoV-2 spike mutations that attenuate monoclonal and serum antibody neutralization[OL].bioRxiv,2020.DOI:10.1101/2020.11.06.372037.
[68]Li Q,Wu J,Nie J,Zhang L,Hao H,Liu S,Zhao C,Zhang Q,Liu H,Nie L,Qin H,Wang M,Lu Q,Li X,Sun Q,Liu J,Zhang L,Li X,Huang W,Wang Y.The Impact of Mutations in SARS-CoV-2 Spike on Viral Infectivity and Antigenicity[J].Cell,2020,182(5):1284-1294 e1289.
[69]Starr T N,Greaney A J,Dingens A S,Bloom J D.Complete map of SARS-CoV-2 RBD mutations that escape the monoclonal antibody LY-CoV555 and its cocktail with LY-CoV016[OL].bioRxiv,2021.DOI:10.1101/2021.02.17.431683.
[70]Van Dorp L,Tan C C,Lam S D,Richard D,Owen C,Berchtold D,Orengo C,Balloux F.Recurrent mutations in SARS-CoV-2 genomes isolated from mink point to rapid host-adaptation[OL].bioRxiv,2020.DOI:10.1101/2020.11.16.384743.
[71]Bayarri-Olmos R,Rosbjerg A,Johnsen L B,Helgstrand C,Bak-Thomsen T,Garred P,Skjoedt M-O.The SARS-CoV-2 Y453F mink variant displays a striking increase in ACE-2 affinity but does not challenge antibody neutralization[OL].bioRxiv,2021.DOI:10.1101/2021.01.29.428834.
[72]Hayashi T,Yaegashi N,Konishi I.Effect of RBD(Y453F)mutation in spike glycoprotein of SARS-CoV-2 on neutralizing IgG affinity[OL].medRxiv,2021.DOI:10.20944/preprints202101.0132.v1.
[73]Broňa B,Viktória H,Kristína B,Viktória?,Lenka R,Evan D P,Pavol?,Boris K,Jozef N,Tomá?V.B.1.258?,a SARS-CoV-2 variant with?H69/?V70 in the Spike protein circulating in the Czech Republic and Slovakia[OL].2021.Available from:https：//virological.org/t/b-1-258-a-sars-cov-2-variant-with-h69-v70-in-the-spike-protein-circulating-in-the-czech-republicand-slovakia/613.
[74]Thomson E C,Rosen L E,Shepherd J G,Spreafico R,da Silva Filipe A,Wojcechowskyj J A,Davis C,Piccoli L,Pascall D J,Dillen J,Lytras S,Czudnochowski N,Shah R,Meury M,Jesudason N,De Marco A,Li K,Bassi J,O’Toole A,Pinto D,Colquhoun R M,Culap K,Jackson B,Zatta F,Rambaut A,Jaconi S,Sreenu V B,Nix J,Zhang I,Jarrett R F,Glass W G,Beltramello M,Nomikou K,Pizzuto M,Tong L,Cameroni E,Croll T I,Johnson N,Di Iulio J,Wickenhagen A,Ceschi A,Harbison AM,Mair D,Ferrari P,Smollett K,Sallusto F,Carmichael S,Garzoni C,Nichols J,Galli M,Hughes J,Riva A,Ho A,Schiuma M,Semple M G,Openshaw P J M,Fadda E,Baillie J K,Chodera J D,Rihn S J,Lycett S J,Virgin H W,Telenti A,Corti D,Robertson D L,Snell G.Circulating SARS-CoV-2spike N439K variants maintain fitness while evading antibody-mediated immunity[J].Cell,2021,184(5):1171-1187.e20.
[75]Wu K,Werner A P,Koch M,Choi A,Narayanan E,Stewart-Jones G B E,Colpitts T,Bennett H,BoyogluBarnum S,Shi W,Moliva J I,Sullivan N J,Graham BS,Carfi A,Corbett K S,Seder R A,Edwards D K.Serum Neutralizing Activity Elicited by mRNA-1273Vaccine-Preliminary Report[J].N Engl J Med,2021.DOI:10.1056/NEJMc2102179.
[76]Singh A,Steinkellner G,Kochl K,Gruber K,Gruber C C.Serine 477 plays a crucial role in the interaction of the SARS-CoV-2 spike protein with the human receptor ACE2[J].Sci Rep,2021,11(1):4320.
[77]Suryadevara N,Shrihari S,Gilchuk P,VanBlargan LA,Binshtein E,Zost S J,Nargi R S,Sutton R E,Winkler E S,Chen E C,Fouch M E,Davidson E,Doranz B J,Chen R E,Shi P Y,Carnahan R H,Thackray L B,Diamond M S,Crowe J E,Jr.Neutralizing and protective human monoclonal antibodies recognizing the N-terminal domain of the SARS-CoV-2spike protein[J].Cell,2021,184(9):2316-2331.e15.
[78]McCallum M,De Marco A,Lempp F A,Tortorici MA,Pinto D,Walls A C,Beltramello M,Chen A,Liu Z,Zatta F,Zepeda S,di Iulio J,Bowen J E,MontielRuiz M,Zhou J,Rosen L E,Bianchi S,Guarino B,Fregni C S,Abdelnabi R,Foo S C,Rothlauf P W,Bloyet L M,Benigni F,Cameroni E,Neyts J,Riva A,Snell G,Telenti A,Whelan S P J,Virgin H W,Corti D,Pizzuto M S,Veesler D.N-terminal domain antigenic mapping reveals a site of vulnerability for SARS-CoV-2[J].Cell,2021,184(9):2332-2347.e16.
[79]McCarthy K R,Rennick L J,Nambulli S,RobinsonMcCarthy L R,Bain W G,Haidar G,Duprex W P.Recurrent deletions in the SARS-CoV-2 spike glycoprotein drive antibody escape[J].Science,2021,371(6534):1139-1142.
[80]Wrapp D,Wang N,Corbett K S,Goldsmith J A,Hsieh C L,Abiona O,Graham B S,McLellan J S.Cryo-EM structure of the 2019-nCoV spike in the prefusion conformation[J].Science,2020,367(6483):1260-1263.
[81]Kemp S A,Collier D A,Datir R P,Ferreira I,Gayed S,Jahun A,Hosmillo M,Rees-Spear C,Mlcochova P,Lumb I U,Roberts D J,Chandra A,Temperton N,Collaboration C-N B C-,Consortium C-G U,Sharrocks K,Blane E,Modis Y,Leigh K,Briggs J,van Gils M,Smith K G C,Bradley J R,Smith C,Doffinger R,Ceron-Gutierrez L,Barcenas-Morales G,Pollock D D,Goldstein R A,Smielewska A,Skittrall J P,Gouliouris T,Goodfellow I G,Gkrania-Klotsas E,Illingworth C JR,McCoy L E,Gupta R K.SARS-CoV-2 evolution during treatment of chronic infection[J].Nature,2021,592(7853):277-282.
[82]Kemp S,Meng B,Ferriera I,Datir R,Harvey W,Collier D,Lytras S,Papa G,,Carabelli A,Kenyon J,Lever A,James L,Robertson D,Gupta R.Recurrent emergence and transmission of a SARS-CoV-2 Spike deletion H69/V70[OL].bioRxiv,2021.DOI:10.1101/2020.12.14.422555.
[83]Hodcroft E B,Domman D B,Snyder D J,Oguntuyo K,Van Diest M,Densmore K H,Schwalm K C,Femling J,Carroll J L,Scott R S,Whyte M M,Edwards M D,Hull N C,Kevil C G,Vanchiere J A,Lee B,Dinwiddie D L,Cooper V S,Kamil J P.Emergence in late 2020 of multiple lineages of SARS-CoV-2 Spike protein variants affecting amino acid position 677[OL].medRxiv,2021.DOI:10.1101/2021.02.12.21251658.
[84]Lubinski B,Tang T,Daniel S,Jaimes J A,Whittaker G R.Functional evaluation of proteolytic activation for the SARS-CoV-2 variant B.1.1.7:role of the P681Hmutation[OL].bioRxiv,2021.DOI:10.1101/2021.04.06.438731.
[85]Voss C,Esmail S,Liu X,Knauer M J,Ackloo S,Kaneko T,Lowes L,Stogios P,Seitova A,Hutchinson A,Yusifov F,Skarina T,Evdokimova E,Loppnau P,Ghiabi P,Haijian T,Zhong S,Abdoh H,Hedley B D,Bhayana V,Martin C M,Slessarev M,Chin-Yee B,Fraser D D,Chin-Yee I,Li S S-C.Epitope-resolved serology test differentiates the clinical outcome of COVID-19 and identifies defects in antibody response in SARS-CoV-2 variants[OL].medRxiv,2021.DOI:10.1101/2021.03.16.21253716.
[86]SARS-CoV-2 Variant Classifications and Definitions[OL].2021.Available from:https：//www.cdc.gov/coronavirus/2019-ncov/cases-updates/variantsurveillance/variant-info.html#Concern.
基本信息:
DOI：10.13242/j.cnki.bingduxuebao.003975
中图分类号:R373
引用信息:
[1]冯晔囡,宋洋,王世文等.全球新型冠状病毒变异株研究进展[J].病毒学报,2021,37(03):695-711.DOI:10.13242/j.cnki.bingduxuebao.003975.
基金信息:
国家重点研发计划(项目号：2018YFC1200305),题目：BSL-4实验室关键设备性能及风险评估与发展战略研究;国家重点研发计划(项目号：2021YFC0863000),题目：新型冠状病毒冷链传播机制和低温消毒技术研究~~