大理大学;上海市浦东新区疾病预防控制中心;复旦大学浦东预防医学研究院;
了解上海市浦东新区不同来源样本中腺病毒的检出情况,初步探讨城市生活污水中腺病毒浓度与病例腺病毒感染的关系。收集2023年7月1日-2024年6月30日上海市浦东新区污水处理厂、监测哨点医院的急性呼吸道感染和急性胃肠道感染样本,用微流体芯片检测污水中的腺病毒和急性呼吸道感染样本中的呼吸道腺病毒,通过实时荧光PCR方法检测腹泻样本中的胃肠道腺病毒。利用不同浓度梯度腺病毒标准品检测结果制作浓度梯度曲线,计算污水样本中腺病毒浓度并与病例检出结果进行分析。研究期间共采集临床样本5097份,其中急性呼吸道感染样本3995份,检出呼吸道腺病毒阳性样本116份,检出率为2.90%(116/3995),急性胃肠道感染样本1102份,检出胃肠道腺病毒阳性样本21份,检出率为1.91%(21/1102)。污水样本357份,检出呼吸道腺病毒342份,检出率为95.80%(342/357),胃肠道腺病毒检出310份,检出率为86.83%(310/357)。城市生活污水中的呼吸道腺病毒和胃肠道腺病毒浓度范围分别在1.09×10~5拷贝/L~2.20×10~7拷贝/L和2.58×10~5拷贝/L~7.10×10~7拷贝/L。污水中胃肠道腺病毒的浓度与病例中胃肠道腺病毒检出率呈现正相关。城市生活污水中的腺病毒浓度与病例检出率之间存在相关性。可以利用污水监测对病例监测进行补充。
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基本信息:
DOI:10.13242/j.cnki.bingduxuebao.004645
中图分类号:R511.8;X832
引用信息:
[1]杨泽,杨晨光,傅慧琴等.城市生活污水中腺病毒浓度与病例腺病毒感染的初步研究[J].病毒学报,2025,41(01):59-70.DOI:10.13242/j.cnki.bingduxuebao.004645.
基金信息:
上海市卫生健康委员会卫生行业临床研究专项(项目号:202240094),题目:致病性人腺病毒与人群感染特征研究; 上海市加强公共卫生体系建设三年行动计划(2023-2025年)(项目号:GWVI-3),题目:超大城市生物安全关键能力和公共卫生实验室网络一体化建设;上海市加强公共卫生体系建设三年行动计划(2023-2025年)重点学科(项目号:GWVI-11.1-02-传染病学),题目:重大传染病早期预警预测、聚集性疫情传播动力学模型、动态监测、干预技术等; 上海市浦东新区卫生健康委员会学科建设计划(项目号:PWZxk2022-25),题目:浦东新区卫生系统重点学科-免疫预防学~~