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以超滤和蔗糖密度梯度超速离心法浓集和纯化猴艾滋病D型逆转录病毒SRV-1感染的Raji细胞培养上清,制备出含SRV-1结构蛋白的抗原制剂。以含有0.02％NaN3的碳酸盐缓冲液作为抗原包被液,不加任何封闭剂,待测血清以含有0.2％NaN3和0.05％Tween-20的PBS稀释,以山羊或兔抗人IgG的辣根过氧化物酶结合物作为酶标抗体,成功地检测出猴血清中抗SRV抗体,并以Western blot最后确诊。
Abstract：Viral structural protein antigens of simian type D retrovirus ( SRV ) were prepared by ultrafiltration and sucrose gradient ultracentrifugation of supernatants of SRV-1 infected Raji cell culture. Carbonate buffer containing 0.02% NaN3 was used for coating the antigens. Without adding proteinous blocking agents to microplate wells, monkey sera examined were diluted with 0.2% NaN3-0.05% Tween-20 in PBS. Conjugates of horseradish peroxidase and goat or rabbit anti-human IgG were applied to ELISA. Antibodies to SRV in macaque sera were successfully determined by the ELISA and were further confirmed by Western blot.
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基本信息:
引用信息:
[1]贲昆龙,陈志斌,田保平,郑永唐.检测猴艾滋病D型逆转录病毒抗体的ELISA技术[J].病毒学报,1992(04):354-359.
基金信息:
云南省应用基础研究基金;; 云南灵长类中心和昆明动物所灵长类生物学联合实验室基金
1992-12-30
1992-12-30