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为了从分子水平上初步探讨我国流行HAdV-4的基因特征以及分子进化规律,为HAdV-4在我国的疫情监测与防控以及疫苗研发提供科学数据,本研究通过对2013～2014年间在陕西、云南、吉林和山东4省发热呼吸道症候群监测病例鼻咽拭子标本中分离得到的HAdV-4毒株,分别进行Penton base,Hexon和Fiber三个靶基因的序列测定,同时下载GenBank数据库中所有我国及全球流行HAdV-4病毒株的上述三基因序列进行比对,构建基因亲缘性关系树,并使用生物信息学分析软件进行基因特征和分子进化分析。结果显示:全球HAdV-4病毒株可划分两个进化分支(HAdV-4p和HAdV-4a),我国自2008年至今流行的HAdV-4病毒株属于HAdV-4a进化分支,与全球优势流行株一致;HAdV-4p和HAdV-4a进化分支病毒株的三个基因序列在时间和空间上均具保守性和稳定性,核苷酸和氨基酸突变主要与不同进化分支密切相关;HAdV-4基于三个基因的估算进化速率分别为3.73×10-5碱基替代/位点/年,1.86×10-5碱基替代/位点/年和4.09×10-5碱基替代/位点/年;基于Fiber基因估算的最近共同祖先(The most recent common ancestor,tMRCA)结果显示,HAdV-4起源于1771年,其中HAdV-4p和HAdV-4a可分别追溯至1933年和1962年。为了进一步积累并完善上述HAdV病毒学监测数据,我国急需建立长期有效的HAdV监测体系。
Abstract：In order to investigate the genetic characteristic and evolution of human adenovirus type 4(HAdV-4),and provide scientific data for HAdV-4 surveillance and control in China's mainland,as well as vaccine research and development,the Penton base,Hexon and Fiber genes of HAdV-4 strains isolated from nasopharyngeal swabs of febrile respiratory syndrome surveillance cases in Shaanxi,Yunnan,Jilin and Shandong Provinces in 2013 and 2014 were sequenced.We also downloaded the sequences of these three genes from GenBank for all HAdV-4 strains from GenBank database to compare and construct a phylogenetic tree,and used bioinformatics analysis software to analyze the genetic characteristics and molecular evolution of these viruses.The results showed that HAdV-4 strains worldwide could be divided into two lineages(HAdV-4 p and HAdV-4 a),and HAdV-4 strains that have been prevalent in China's mainland since 2008 belong to HAdV-4 a,which was consistent with the global dominant strains;Both HAdV-4 p and HAdV-4 astrains showed significant conservation and stability of the sequences within the genome across time and space,and nucleotide and amino acid mutations are closely related to different lineages;The estimated evolution rates of HAdV-4 based on the three genes were 3.73×10-5 substitution/site/year,1.86×10-5 substitution/site/year and 4.09×10-5 substitution/site/year,respectively.The most recent common ancestor(tMRCA)results based on Fiber gene estimates showed that HAdV-4 originated in 1771,and HAdV-4 p and HAdV-4 acould be traced back to 1933 and 1962,respectively.To further accumulate and improve the above HAdV viro,it is in urgent need to establish a long-term effective HAdV monitoring system in China.
[1]Davison A J,Benko M,and Harrach B.Genetic content and evolution of adenoviruses[J].J Gen Virol,2003,84(Pt 11):2895-2908.
[2]Madisch I,Harste G,Pommer H,Heim A.Phylogenetic analysis of the main neutralization and hemagglutination determinants of all human adenovirus prototypes as a basis for molecular classification and taxonomy[J].J Virol,2005,79(24):15265-15276.
[3]Chroboczek J,Ruigrok R W,and Cusack S.Adenovirus fiber[J].Curr Top Microbiol Immunol,1995,199(Pt1):163-200.
[4]Lion T.Adenovirus infections in immunocompetent and immunocompromised patients[J].Clin Microbiol Rev,2014,27(3):441-462.
[5]Scott M K,Chommanard C,Lu X,Appelgate D,Grenz L,Schneider E,Gerber S I,Erdman D D,Thomas A.Human Adenovirus Associated with Severe Respiratory Infection,Oregon,USA,2013-2014[J].Emerg Infect Dis,2016,22(6):1044-1051.
[6]Huang G,Yu D,Mao N,Zhu Z,Zhang H,Jiang Z,Li H,Zhang Y,Shi J,Zhang S,Wang X,Xu W.Viral etiology of acute respiratory infection in Gansu Province,China,2011[J/OL].PLoS One,2013,8(5):e64254.
[7]Sanchez J L,Binn L N,Innis B L,Reynolds R D,Lee T,Mitchell-Raymundo F,Craig S C,Marquez J P,Shepherd G A,Polyak C S,Conolly J,Kohlhase K F.Epidemic of adenovirus-induced respiratory illness among US military recruits:epidemiologic and immunologic risk factors in healthy,young adults[J].J Med Virol,2001,65(4):710-718.
[8]Ghebremedhin,B,Human adenovirus:Viral pathogen with increasing importance[J].Eur J Microbiol Immunol(Bp),2014,4(1):26-33.
[9]Kajon A E,Moseley J M,Metzgar D,Huong H S,Wadleigh A,Ryan M A,Russell K L.Molecular epidemiology of adenovirus type 4infections in US military recruits in the postvaccination era(1997-2003)[J].J Infect Dis,2007,196(1):67-75.
[10]Lynch B L,Dean J,Brady D,De Gascun C.Adenovirus type 4respiratory infections among civilian adults,northeastern United States,2011-2015[J].Emerg Infect Dis,2018,24(7):1392-1393.
[11]Binder A M,Biggs H M,Haynes A K,Chommanard C,Lu X,Erdman D D,Watson J T,Gerber S I.Human adenovirus surveillance-United States,2003-2016[J].MMWR Morb Mortal Wkly Rep,2017,66(39):1039-1042.
[12]Niang M N,Diop N S,Fall A,Kiori D E,Sarr F D,Sy S,Goudiaby D,Barry M A,Fall M,Dia N.Respiratory viruses in patients with influenza-like illness in Senegal:Focus on human respiratory adenoviruses[J/OL].PLoS One,2017,12(3):e0174287.
[13]Ariga T,Shimada Y,Ohgami K,Tagawa Y,Ishiko H,Aoki K,Ohno S.New genome type of adenovirus serotype 4caused nosocomial infections associated with epidemic conjunctivitis in Japan[J].J Clin Microbiol,2004.42(8):3644-3648.
[14]Aoki,K,Tagawa Y.A twenty-one year surveillance of adenoviral conjunctivitis in Sapporo,Japan[J].Int Ophthalmol Clin,2002,42(1):49-54.
[15]Gopalkrishna V,Ganorkar N N,Patil P R.Identification and molecular characterization of adenovirus types(HAdV-8,HAdV-37,HAdV-4,HAdV-3)in an epidemic of keratoconjunctivitis occurred in Pune,Maharashtra,Western India[J].J Med Virol,2016,88(12):2100-2105.
[16]Guo L,Gonzalez R,Zhou H,Wu C,Vernet G,Wang Z,Wang J.Detection of three human adenovirus species in adults with acute respiratory infection in China[J].Eur J Clin Microbiol Infect Dis,2012,31(6):1051-1058.
[17]Li J,Lu X,Jiang B,Du Y,Yang Y,Qian H,Liu B,Lin C,Jia L,Chen L,Wang Q.Adenovirus-associated acute conjunctivitis in Beijing,China,2011-2013[J].BMC Infect Dis,2018,18(1):135.
[18]Tamura K,Peterson D,Peterson N,Stecher G,Nei M,Kumar S.MEGA5:molecular evolutionary genetics analysis using maximum likelihood,evolutionary distance,and maximum parsimony methods[J].Mol Biol Evol,2011,28(10):2731-2739.
[19]Drummond,A J,Rambaut A.BEAST:Bayesian evolutionary analysis by sampling trees[J].BMC Evol Biol,2007,7:214.
[20]Tian X,Qiu H,Zhou Z,Wang S,Fan Y,Li X,Chu R,Li H,Zhou R,Wang H.Identification of acritical and conformational neutralizing epitope in human adenovirus type 4hexon[J/OL].J Virol,2018,92(2):e01643-17.
[21]Purkayastha A,Su J,McGraw J,Ditty S E,Hadfield T L,Seto J,Russell K L,Tibbetts C,Seto D.Genomic and bioinformatics analyses of HAdV-4vac and HAdV-7vac,two human adenovirus(HAdV)strains that constituted original prophylaxis against HAdV-related acute respiratory disease,a reemerging epidemic disease[J]. J Clin Microbiol, 2005, 43(7):3083-3094.
[22]Wadell,G.Molecular epidemiology of human adenoviruses[J].Curr Top Microbiol Immunol,1984,110:191-220.
[23]Fox,J P,Hall C E,Cooney M K.The Seattle Virus Watch.VII.Observations of adenovirus infections[J].Am J Epidemiol,1977,105(4):362-386.
[24]Hoke,C H,Snyder C E.History of the restoration of adenovirus type 4and type 7vaccine,live oral(Adenovirus Vaccine)in the context of the department of defense acquisition system[J].Vaccine,2013,31(12):1623-1632.
[25]Gray G C,Goswami P R,Malasig M D,Hawksworth A W,Trump D H,Ryan M A,Schnurr D P.Adult adenovirus infections:loss of orphaned vaccines precipitates military respiratory disease epidemics.For the adenovirus surveillance group[J].Clin Infect Dis,2000,31(3):663-670.
[26]Blasiole D A,Metzgar D,Daum L T,Ryan M A,Wu J,Wills C,Le C T,Freed N E,Hansen C J,Gray G C,Russell K L.Molecular analysis of adenovirus isolates from vaccinated and unvaccinated young adults[J].J Clin Microbiol,2004,42(4):1686-1693.
[27]Dehghan S,Seto J,Liu E B,Walsh M P,Dyer D W,Chodosh J,Seto D.Computational analysis of four human adenovirus type 4genomes reveals molecular evolution through two interspecies recombination events[J].Virology,2013,443(2):197-207.
[28]许晶,岳姝婷,马萍,张蕾,史伟,徐艺,李慎,关路媛,王敬军,余鹏博.西安市流感样病例中人腺病毒感染特点分析[J].中华实验和临床病毒学杂志,2017,31(3):218-222.
[29]罗小芳,张兵,段招军,刘沁,谢志萍.2013-2014年长沙急性下呼吸道感染住院儿童腺病毒的分子流行特征[J].中华实验和临床病毒学杂志,2015,29(6):505-509.
[30]刘滕颖,吕星,黄达娜,房师松,吴春利,阳帆,王昕,伍伟华,彭博,张仁利.2011-2012年深圳市呼吸道腺病毒分子分型及其流行特征[J].热带医学杂志,2014,14(1):12-15.
[31]李红,刘高山,石燕,牛培华,姜双应,徐莉立,陆柔剑,赵生仓,谭文杰.2012-2015年青海省44例发热呼吸道症候群病例人腺病毒感染特征分析[J].中华预防医学杂志,2017,51(7):650-652.
[32]Narra R,Bono P,Zoccoli A,Orlandi A,Piconi S,Grasselli G,Crotti S,Girello A,Piralla A,Baldanti F,Lunghi G.Acute respiratory distress syndrome in adenovirus type 4pneumonia:A case report[J].J Clin Virol,2016,81:78-81.
[33]Seto J,Walsh MP,Metzgar D,Seto D.Computational analysis of adenovirus serotype 5(HAdV-C5)from an HAdV coinfection shows genome stability after 45years of circulation[J].Virology,2010,404(2):180-186.
[34]Mahadevan P,Seto J,Tibbetts C,Seto D.Natural variants of human adenovirus type 3provide evidence for relative genome stability across time and geographic space[J].Virology,2010,397(1):113-118.
[35]Lu Q B,Tong Y G,Wo Y,Wang H Y,Liu E M,Gray G C,Liu W,Cao W C.Epidemiology of human adenovirus and molecular characterization of human adenovirus 55in China,2009-2012[J].Influenza Other Respir Viruses,2014,8(3):302-308.
[36]毛乃颖,朱贞,雷振强,李岩,黄芳,尹洁,陈萌,向星宇,李红,唐浏英,崔爱利,李忠,刘倜,许文波.新型人腺病毒55型在中国10年的持续流行及其基因进化分析[J].中华实验和临床病毒学杂志,2018,32(2):124-129.
基本信息:
DOI：10.13242/j.cnki.bingduxuebao.003463
中图分类号:R373.1
引用信息:
[1]李红,毛乃颖,余鹏博,等.2013～2014年我国4省急性呼吸道感染人腺病毒4型的基因特征分析[J].病毒学报,2018,34(06):850-859.DOI:10.13242/j.cnki.bingduxuebao.003463.
基金信息:
“十三五”国家科技重大专项(项目号:2018ZX10713002),题目:病毒性传染病病原谱和病毒基因变异变迁规律研究;“十三五”国家科技重大专项(项目号:2018ZX10713001-003),题目:病毒性自然疫源性传染病症候群病原谱构成及流行规律研究;; 艾滋病、肝炎等传染病防治“十三五”科技重大专项(项目号:2017ZX10104001),题目:基于全基因组的病毒网络化监测和溯源技术体系研究;; 国家重点研发计划(项目号:2017YFC1200303),题目:高等级生物设施重大事故模拟仿真与风险评估关键技术研究;国家重点研发计划(项目号:2016YFC1200905),题目:重要新发突发病原体应急处置方案与规范研究~~
2018-11-28
2018-11-28
2018-11-28