| 401 | 22 | 122 |
| 下载次数 | 被引频次 | 阅读次数 |
2020年云南发现了首例人感染G4基因型欧亚类禽H1N1(EA H1N1)猪流感病毒，针对分离到的A/YunnanMengzi/1462/2020(H1N1v)病毒分析其血凝素（HA）和神经氨酸酶（NA）氨基酸变异位点、生物学耐药、受体亲和力及抗原性变异特征，并对2020-2021年度季节性流感疫苗对该病毒的交叉保护效果进行评价。结果显示，EA H1N1猪流感病毒A/Yunnan-Mengzi/1462/2020(H1N1v)与世界卫生组织推荐的疫苗株A/Hunan/42443/2015(H1N1v)HA和NA氨基酸位点同源性分别为97.9%和97.4%;A/Yunnan-Mengzi/1462/2020(H1N1v)病毒对神经氨酸酶抑制剂敏感，以结合人流感病毒唾液酸受体α2,6为主，与疫苗株抗原性存在8倍以上差异。接种季节性流感疫苗的儿童、成人和老年组人群针对季节性流感疫苗株A/Guangdong-Maonan/SWL1536/2019(H1N1pdm)抗体滴度≥40者占比分别为80.0%、76.7%和63.3%；而对A/Yunnan-Mengzi/1462/2020(H1N1v)抗体滴度仅为13.3%、26.7%和6.7%。本研究提示接种季节性流感疫苗后产生的抗体对G4基因型EA H1N1病毒不能提供有效保护，该病毒与目前EA H1N1病毒疫苗株在氨基酸位点和抗原性上存在显著差异，并且保持了结合人流感病毒唾液酸受体的特性，研究结果提示需要构建新的疫苗株。
Abstract：In 2020,the first case of human infection by the G4 genotype Eurasian avian-like H1N1(EA H1N1)swine influenza virus,A/Yunnan-Mengzi/1462/2020(H1N1v),was found in Yunnan Province,China. We analyzed the characteristics of the amino-acid sequences of hemagglutinin(HA) and neuraminidase(NA),biological resistance,receptor affinity and antigenicity of this virus strain. The antiserum from the 2020– 2021 seasonal influenza vaccinated population was used to evaluate the cross-protection effect. The homology of HA and NA between of A/Yunnan-Mengzi/1462/2020(H1N1v) and the candidate vaccine virus(CVV),A/Hunan/42443/2015(H1N1v),as recommended by the World Health Organization,was 97.9% and 97.4%,respectively. The A/Yunnan-Mengzi/1462/2020(H1N1v) virus was sensitive to NA inhibitors and bound mainly to the α2,6 sialic acid receptor of the human influenza virus. The hemagglutination inhibition titer between the A/Yunnan-Mengzi/1462/2020(H1N1v) virus and CVV was >8-fold. The proportion of children,adults and elderly people vaccinated with the seasonal influenza vaccine with antibody titer ≥40 against the seasonal influenza vaccine strain A/Guangdong-Maonan/SWL1536/2019(H1N1 pdm)was 80.0%,76.7% and 63.3%,respectively,and the antibody titer against A/Yunnan-Mengzi/1462/2020(H1N1v)was only 13.3%,26.7% and 6.7%,respectively. These results suggest that vaccination with a seasonal influenza vaccine did not provide effective protection against the G4 genotype EA H1N1 isolated in our study. There are significant differences in amino-acid sites and antigenicity between this virus and the current CVV for EA H1N1.A new CVV should be prepared. Furthermore,the virus maintains the properties of binding to the α2,6 sialic acid receptor.
[1] Pensaert M, Ottis K, Vandeputte J, Kaplan MM,Bachmann PA. Evidence for the natural transmission of influenza A virus from wild ducks to swine and its potential importance for man[J]. Bull World Health Organ,1981,59(1):75-78.
[2] Vijaykrishna D,Smith GJD,Pybus OG,Zhu H,Bhatt S,Poon LLM,Riley S,Bahl J,Ma SK,Cheung CL,Perera RAPM,Chen H,Shortridge KF,Webby RJ,Webster RG, Guan Y, Peiris JSM. Long-term evolution and transmission dynamics of swine influenza A virus[J/OL]. Nature,2011,473(7348):519-522.DOI:10.1038/nature10004.
[3] Smith GJD, Vijaykrishna D, Bahl J, Lycett SJ,Worobey M, Pybus OG, Ma SK, Cheung CL,Raghwani J,Bhatt S,Peiris JSM,Guan Y,Rambaut A. Origins and evolutionary genomics of the 2009 swineorigin H1N1 influenza A epidemic[J/OL]. Nature,2009, 459(7250):1122-1125. DOI:10.1038/nature08182.
[4] Vijaykrishna D, Poon LLM, Zhu HC, Ma SK, Li OTW,Cheung CL,Smith GJD,Peiris JSM,Guan Y.Reassortment of pandemic H1N1/2009 influenza A virus in swine[J/OL]. Science,2010,328(5985):1529-1529.DOI:10.1126/science.1189132.
[5] Yang H,Chen Y,Qiao C,He X,Zhou H,Sun Y,Yin H,Meng S,Liu L,Zhang Q,Kong H,Gu C,Li C,Bu Z,Kawaoka Y,Chen H. Prevalence,genetics,and transmissibility in ferrets of eurasian avian-like H1N1swine influenza viruses[J/OL]. Proc Natl Acad Sci,2016, 113(2):392-397. DOI:10.1073/pnas.1522643113.
[6] Sun H,Xiao Y,Liu J,Wang D,Li F,Wang C,Li C,Zhu J,Song J,Sun H,Jiang Z,Liu L,Zhang X,Wei K,Hou D,Pu J,Sun Y,Tong Q,Bi Y,Chang KC,Liu S,Gao GF,Liu J. Prevalent eurasian avian-like H1N1 swine influenza virus with 2009 pandemic viral genes facilitating human infection[J/OL]. Proc Natl Acad Sci, 2020, 117(29):17204-17210. DOI:10.1073/pnas.1921186117.
[7] Qi X,Cui L,Jiao Y,Pan Y,Li X,Zu R,Huo X,Wu B,Tang F,Song Y,Zhou M,Wang H,Cardona CJ,Xing Z. Antigenic and genetic characterization of a european avian-like H1N1 swine influenza virus from a boy in China in 2011[J/OL]. Arch Virol,2013,158(1):39-53. DOI:10.1007/s00705-012-1423-7.
[8] Zhu W,Zhang H,Xiang X,Zhong L,Yang L,Guo J,Xie Y,Li F,Deng Z,Feng H,Huang Y,Hu S,Xu X,Zou X,Li X,Bai T,Chen Y,Li Z,Li J,Shu Y.Reassortant eurasian avian-like influenza A(H1N1)virus from a severelyⅢchild,hunan province,China,2015[J/OL].Emerg Infect Dis,2016,22(11):1930-1936.DOI:10.3201/eid2211.160181.
[9]黄维娟，谭敏菊，李希妍，成艳辉，隗合江，肖宁，赵翔，曾晓旭，王大燕. 2013-2017年中国大陆流行的A(H3N2)亚型流感病毒对神经氨酸酶抑制剂的敏感性分析[J/OL].病毒学报，2018,34(6):793-799. DOI:10.13242/j.cnki.bingduxuebao.003465.
[10]Liu LQ,Li Z,Zhou JF,Lu J,Li XY,Liu J,Xiao N,Wang DY. The efect of single amino acid substitution at position 220 in the hemagglutinin glycoprotein on avian infuenza H7N9 candidate vaccine virus[J/OL]. Virus Genes,2021,2(57):164-171. DOI:10.1007/s11262-021-01827-y.
[11]Liu M,Zhao X,Hua S,Du XJ,Peng YS,Li XY,Lan Y,Wang DY,Wu AP,Shu YL,Jiang TJ. Antigenic patterns and evolution of the human influenza A(H1N1)virus[J/OL]. Scientific Reports,2015,9(5):14171-14178. DOI:10.1038/srep14171.
[12]Influenza at the human-animal interface summary and assessment, 15 April 2021. https：//www. who. int/emergencies/situation-reports.
[13]Zhou NN, Senne DA, Landgraf JS, Swenson SL,Erickson G,Rossow K,Liu L,Yoon K,Krauss S,Webster R G. Genetic reassortment of avian,swine,and human influenza A viruses in American pigs[J/OL].J Virol,1999,73(10):8851-8856. DOI:10.1128/JVI.73.10.8851-8856.1999.
[14]Zhu H,Zhou B,Fan X,Lam TTY,Wang J,Chen A,Chen X, Chen H, Webster RG, Webby R, Peiris JSM, Smith DK, Guan Y. Novel reassortment of eurasian avian-like and pandemic/2009 influenza viruses in swine:infectious potential for humans[J/OL]. J Virol,2011,85(20):10432-10439. DOI:10.1128/JVI.05352-11.
[15]Nelli RK, Kuchipudi SV, White GA, Perez BB,Dunham SP,Chang KC. Comparative distribution of human and avian type sialic acid influenza receptors in the pig[J/OL].BMC Vet Res,2010,6(4):2-9. DOI:10.1186/1746-6148-6-4.
[16]Li TCM,Chan MCW,Lee N. Clinical implications of antiviral resistance in influenza[J/OL]. Viruses,2015,7(9):4929-4944. DOI:10.3390/v7092850.
[17]Seibert CW, Rahmat S, Krammer F, Palese P,Bouvier NM. Efficient transmission of pandemic H1N1influenza viruses with high-level oseltamivir resistance[J/OL]. J Virol,2012,86(9):5386-5389. DOI:10.1128/JVI.00151-12.
[18]Suttie A, Deng YM, Greenhill AR, Dussart P,Horwood PF, Karlsson EA. Inventory of molecular markers affecting biological characteristics of avian influenza A viruses[J/OL].Virus Genes,2019,55(6):739-768. DOI:10.1007/s11262-019-01700-z.
基本信息:
DOI：10.13242/j.cnki.bingduxuebao.004028
中图分类号:R373.13
引用信息:
[1]李梓,赵晓南,黄维娟,等.云南省首例人感染G4基因型欧亚类禽H1N1猪流感病毒病原学特征分析[J].病毒学报,2022,38(02):290-297.DOI:10.13242/j.cnki.bingduxuebao.004028.
基金信息:
国家科技重大专项(项目号：2020ZX10001-016-004-001)，题目：人感染禽流感病毒潜在耐药突变及对致病性影响~~
2021-05-31
2021
2021-06-20
2021-08-17
2021
1
2021-08-18
2021-08-18
2021-08-18