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甲型流感病毒易发生基因的突变或重配,曾多次引起世界性大流行,导致严重的疾病负担。为分析2017～2018监测年度我国大陆流行的A(H1N1)pdm09亚型流感病毒的抗原性和基因特性变异情况以及与疫苗株的匹配性,用免疫雪貂后的抗血清进行红细胞凝集抑制试验,对1 079株A(H1N1)pdm09亚型流感病毒进行抗原分析,用深度测序法对其中121株流感病毒进行序列测定。抗原分析结果显示,2017～2018年检测的95%以上的A(H1N1)pdm09亚型流感病毒与疫苗株A/Michigan/45/2015抗原性类似。序列分析结果显示,2017～2018年流行的A(H1N1)pdm09亚型流感病毒的血凝素(Hemagglutinin,HA)基因分为6B.1和6B.2两个分支,绝大部分毒株属于6B.1分支,少数毒株属于6B.2分支,两个分支间毒株的抗原性无明显差异。因此,上述监测结果表明,2017～2018年我国大陆流行的A(H1N1)pdm09亚型流感病毒与WHO推荐的北半球的疫苗组分匹配。
Abstract：Influenza A virus is prone to genetic mutation or reassortment,and has caused worldwide pandemics many times,leading to a serious disease burden. Antigenic characteristics of 1,079 influenza A(H1N1)pdm09 viruses were assessed with post-infection ferret antisera in hemagglutination inhibition(HI)assays. Also,121 viruses were sequenced by next-generation sequencing for the antigenic and genetic characterization of influenza A(H1N1)pdm09 viruses,and matched with a vaccine virus in China's mainland during the 2017～2018 influenza season. We found that > 95% of detected influenza A(H1N1)pdm09 viruses during the 2017～2018 influenza season were antigenically similar to the vaccine virus A/Michigan/45/2015. Sequencing showed that the HA gene of influenza A(H1N1)pdm09 viruses could be divided into 6 B.1 and 6 B.2 clades during the 2017～2018 influenza season. Most of the viruses belonged to the 6 B.1 clade and a few belonged to the 6 B.2 clade. There was no significant difference in antigenicity between the two clades. These data suggested that circulating influenza A(H1N1)pdm09 viruses in China's mainland during the 2017～2018 influenza season matched the vaccine component in the northern hemisphere recommended by the World Health Organization.
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基本信息:
DOI：10.13242/j.cnki.bingduxuebao.003543
中图分类号:R373.13
引用信息:
[1]成艳辉,刘佳,谭敏菊,等.2017～2018监测年度中国大陆A(H1N1)pdm09亚型流感病毒抗原性和基因特性分析[J].病毒学报,2019,35(03):431-439.DOI:10.13242/j.cnki.bingduxuebao.003543.
基金信息:
“十三五”国家科技重大专项“艾滋病和病毒性肝炎等重大传染病防治”(项目号:2018ZX10711001),题目:病毒感染高通量快速检测和应急筛检技术研究~~
2019-05-24
2019-05-24
2019-05-24