| 139 | 4 | 50 |
| 下载次数 | 被引频次 | 阅读次数 |
采用RT—PCR方法对口蹄疫病毒O/NY00株基因组L片段进行了分子克隆和序列测定。结果表明:获得的O/NY00株基因组L片段长7805nt,其中包括715at的5’非编码区和6999nt的多聚蛋白编码区。将O/NY00株与其它参考毒株进行序列比较,结果显示:O/NY00株与O/SKR/2000、O/UKG/3/2001遗传关系最近,而与其它毒株遗传关系较远,属于O型口蹄疫ME-SA拓扑型PanAsia株的成员。与Cathay拓扑型的代表毒株O/TAW/97比较,两者在主要抗原位点1和位点3呈现出明显的以拓扑型为特征的氨基酸差别,提示两拓扑型毒株之间有较高的抗原差异。
Abstract：The L fragment of the genome of foot-and-mouth disease virus(FMDV) O/NY00 isolate was cloned by RT-PCR,and sequenced.The results showed that L genomic fragment of O/NY00 isolate is 7 731nt in length including partial 5' UTR and complete polyprotein coding region with 715nt and 6 999nt respectively .Comparison among O/NY00 and other reference isolates indicated that O/NY00,O/SKR/2000 and O/UKG/ 3/2001 may come from the same ancestor, and O/NY00 should be classified into PanAsia strain of FMDV serotype O ME - SA topotype. Furthermore, comparison of deduced amino acid residues at the antigenic sites with O/TAW/97,a typical isolate of Cathay topotype,showed there are obviouslly topotypical amino acid differences at Site 1 and Site 3, thus, suggesting the less antigenic relationships between these two topotype isolates.
[1]张显升,刘在新,赵启祖,等．口蹄疫病毒基因组RNA结构与功 能研究进展[J]．病毒学报,2001,17(4):375—380．
[2] Samuel A R, Knowles N J. Foot - and - mouth disease type O viruses exhibit genetically and geographically distinct evolutionary lineages (topotypes)[J].J Gen Virol.2001,82:609-621.
[3] Knowles N J, Samuel A R, Davies P R, et al. Emergence of a Pandemic Strain of Foot - and - Mouth Disease Virus Serotype O. Report of the Session of the Research Group of the Standing Technical Committee of the European Commission for the Control of Foot-and-mouth Diseae, Borovets, Bulgaria, 5-8 September 2000 (Appendix 1) .FAO,Rome,pp.20-31.
[4]Knowles N J,Samuel A R.Molecular epidemiology of foot-and-mouth diseae virus[J] .Virus Res,2003,91:65-80.
[5]Mason P W,Grubman M J,Barry B.Molecular basis of pathogenesis of FMDV[J].Virus Res, 2003,91:9-32.
[6]Clarke B E,Brown A L,Currey K M,et al.Potential secondary and tertiary structures in the genomic RNA of foot-and-mouth disease virus[J] .Nucleic Acids Res, 1987,15:7067 - 7079.
[7]Crowther J R,Farias S,Carpenter W C,et al. Identification of a fifth neutralizable site on type O foot-and-mouth disease virus following characterization of single and quintuple monoclonal antibody escape mutants[J].J Gen Virol,1993,74:1547 - 1553.
[8] Kitson J D, McCahon D, Belsham G J. Sequence analysis of monoclonal antibody resistant mutants of type O foot-and-mouth disease virus: evidence for the involvement of the three surface exposed capsid proteins in four antigenic sites[J] .Virology, 1990,179:26 - 34.
[9]Beard C W, Mason P W.Genetic determinant of altered virulence of Taiwanese foot-and-mouth disease virus[J] .J Virol,2000,74:987 -991.
[10]Chang H K, Young J K,Won I K,et al.Molecular characterization of foot-and-mouth disease virus O/SKR/2000 [J] .Virus Res, 2002, 90:15-22.
基本信息:
DOI：10.13242/j.cnki.bingduxuebao.001523
中图分类号:S852.65
引用信息:
[1]郑敏,金宁一,张洪勇,刘棋,韦婷,郭建刚,李华明,尹革芬,鲁会军.口蹄疫病毒O/NY00株基因组L片段的克隆及其基因特征[J].病毒学报,2005(02):124-130.DOI:10.13242/j.cnki.bingduxuebao.001523 .
基金信息:
国家863计划资助项目(2001AA213071) 吉林省科技厅重点项目(20040202—1)
2005-03-20
2005-03-20