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本文旨在监测广西南宁猪场猪繁殖与呼吸综合征病病毒（Porcine reproductive and respiratory syndrome virus,PRRSV）的流行情况。采集2022年广西壮族自治区南宁市4个不同规模化猪场的9322份血清样品、1348份睾丸液、402份精液样品、3083份唾液样品和787份环境样品，使用qRT-PCR方法对样品中的病原进行检测，同时扩增PRRSV阳性样品的ORF5和NSP2基因；测序后利用DNAStar软件进行遗传进化分析、氨基酸序列比对分析，并对ORF5基因的N-糖基化位点进行预测。从PRRSV阳性样品中成功获得23条ORF5基因序列和18条NSP2基因序列，基于ORF5基因遗传进化分析属于PRRSV美洲型，其中NADC30-like毒株13株，NADC34-like毒株9株，VR2332-Like毒株1株。对ORF5氨基酸进行多序列比对显示，23株毒株无插入或缺失，但都存在位点突变。基于NSP2基因遗传进化分析显示为PRRSV美洲型，对NSP2氨基酸进行多序列比对显示，18株PRRSV存在1+19个氨基酸的缺失，与NADC30毒株NSP2氨基酸对应位置缺失模式一致。结果表明，该地区猪场主要流行NADC30-Like和NADC34-Like毒株，PRRSV不同流行毒株存在一定的遗传差异，本研究为广西PRRS防控和疫苗选用提供了科学依据。
Abstract：The purpose of this study is to monitor the prevalence of porcine reproductive and respiratory syndrome virus(PRRSV) in pig farms in Nanning, Guangxi, China. A total of 9322 serum samples, 1348testicular fluid samples, 402 seminal samples, 3083 saliva samples and 787 environmental samples were collected from four large-scale pig farms in Nanning, Guangxi, China in 2022. The pathogens in samples were detected with the qRT-PCR technique and simultaneously the ORF5 and NSP2 genes in PRRSV-positive samples were amplified. Then, genetic evolutionary analysis and amino acid sequence alignment analysis were performed using DNA Star software, and the N-glycosylation sites of the ORF5 gene were predicted after sequencing. 23 PRRSV ORF5 gene sequences and 18 NSP2 gene sequences were successfully obtained from the positive samples. According to the genetic evolutionary analysis, the ORF5 genes are PRRSV North American type strains, including 13 NADC30-like strains, 9 NADC34-like strains and 1 VR2332-like strain.Multiple sequence alignment of the ORF5 amino acid sequences shows no insertions or deletions but site mutations in all 23 strains. Genetic evolutionary analysis of NSP2 gene also indicates the presence of the PRRSV American type. Multiple sequence alignment of the NSP2 amino acid sequences reveals a deletion of 1+19 amino acids in the 18 PRRSV strains, consistent with the deletion pattern in the corresponding NSP2amino acid positions of NADC30 strain. The results show NADC30-like and NADC34-like strains as the prevalent strains in the studied pig farms and genetic differences among PRRSV strains, providing scientific evidences for PRRS prevention and vaccine selection in Guangxi.
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基本信息:
DOI：10.13242/j.cnki.bingduxuebao.004603
中图分类号:S852.651
引用信息:
[1]李瑞雪,李承辉,陈俭,等.广西南宁猪繁殖与呼吸综合征病毒流行毒株ORF5和NSP2基因的遗传变异分析[J].病毒学报,2025,41(01):173-183.DOI:10.13242/j.cnki.bingduxuebao.004603.
基金信息:
国家重点研发计划：（项目号：2022YFD1800304-06）,题目：PRRSV继发感染过程中黏附相关宿主蛋白的作用机制; 广西南宁市科技局重点研发计划：（项目号：20232059）,题目：规模化母猪场主要腹泻病原综合防控体系研究与示范; 广西金陵研究院：（项目号：K4050722222）,题目：金陵法系后备母猪蓝耳病血清驯化综合防控与示范
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