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为了解2011~2012年河北省急性弛缓性麻痹(Acute flaccid paralysis,AFP)病例中非脊髓灰质炎肠道病毒(Non-polio enterovirus,NPEV)的血清型分布,探讨这些病毒与AFP的关系,本研究按照世界卫生组织要求,对河北省AFP病例粪便标本利用人横纹肌肉瘤(Human rhabdomyosarcoma,RD)细胞和转人脊髓灰质炎病毒受体的小鼠肺(Mouse cell line expressing the gene for the human cellular receptor for poliovirus,L20B)细胞进行病毒分离。筛检出NPEV毒株后,进行VP1区核苷酸序列测定,使用分子定型方法对NPEV进行血清型别鉴定。结果显示,在2011~2012年AFP病例中分离到的82株NPEV中共鉴定出A组人肠道病毒(HEV-A)42株(55.3%),包含4个血清型;B组人肠道病毒(HEV-B)34株(44.7%),包含13个血清型别,未鉴定出C组和D组人肠道病毒。另外还有2株腺病毒,4株未定型NPEV。HEV-A组中又以肠道病毒71型(EV-A71)为主要型别,在HEV-A中比例高达85.7%。2011~2012年河北省AFP病例中分离到的NPEV以HEV-A居多,尤其是EV-A71,为AFP病例中的优势毒株。
Abstract：This study aims to investigate the serotype distribution of non-polio enterovirus(NPEV)isolated from patients with acute flaccid paralysis(AFP)during 2011-2012in Hebei Province,China and to analyze the relationship between these viruses and AFP.NPEV strains were isolated from the stool specimens from AFP cases in Hebei using human rhabdomyosarcoma cells(RD)and the mouse cell line expressing the gene for the human cellular receptor for poliovirus(L20B)according to the WHO requirements.The nucleotide sequence of VP1region was determined,and the serotypes of NPEV were identified by molecular typing.The results showed that among the 82strains of NPEV isolated from the AFP cases during 2011-2012,42isolates(55.3%)were identified as human enterovirus A(HEV-A),which were classified into 4 serotypes,34(44.7%)as human enterovirus B(HEV-B),which were classified into 13serotypes,2as adenovirus,and 4were untyped;human enteroviruses C and D were not found in these cases.Enterovirus A71(EV-A71)was the main type of HEV-A,accounting for 85.7%of all HEV-A strains.HEV-A,especially EV-A71,was predominant among the NPEV strains isolated from AFP patients during 2011-2012in Hebei Province.
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基本信息:
DOI：10.13242/j.cnki.bingduxuebao.000003
中图分类号:R181.3
引用信息:
[1]崔志强,赵娜,张俊棉,等.2011~2012年河北省急性弛缓性麻痹病例中非脊髓灰质炎肠道病毒血清型的分布[J].病毒学报,2014,30(01):33-36.DOI:10.13242/j.cnki.bingduxuebao.000003.
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2014-01-24
2014-01-24