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目的 为了解山东省诺如病毒遗传多样性特征，为诺如病毒防控提供科学依据。方法 我们于2024年在济南市、烟台市和临沂市开展为期一年的诺如病毒横断面污水监测，按月采集污水样本，使用阴离子膜法进行浓缩，提取核酸对诺如病毒VP1编码区进行RT-PCR扩增，阳性产物进行下一代测序，使用CLC 24.0软件进行从头组装和有参组装，使用BioEdit和MEGA软件对优势型别GII.17进行相似性和系统发生学分析。结果 研究期间共采集36份样本，对扩增产物进行下一代测序和分析，共鉴定出21种基因型。包括9种GI(GI.1～GI.9)、11种GII(GII.1～GII.4、GII.6～GII.9、GII.12、GII.13、GII.17)基因型以及GIX.1基因型。GI.2和GII.17是监测地区共同优势基因型，其中GII.17是检出频率最高的型别。系统发育显示三地市污水中的GII.17序列具有多个不同的分支谱系，提示山东省存在多个分支的共循环。结论 本研究发现山东省污水中的诺如病毒存在高度的遗传多样性，不同基因型在人群中的共同循环传播，且不同监测点地区之间在优势型别构成、流行动态等方面存在差异，研究结果进一步证实了环境监测是探索人群中诺如病毒区域流行的有效手段。
Abstract：Objective To characterize the genetic diversity of Norovirus in Shandong Province, China and provide a scientific basis for viral prevention and control. Methods A one-year cross-sectional sewage monitoring for norovirus was conducted in Jinan, Yantai and Linyi of Shandong Province, China in 2024, with sewage samples collected monthly. The samples were thickened using the anion membrane adsorption–elution method. Then, nucleic acid was extracted, RT-PCR was performed to amplify the VP1 coding region, and the positive products were subjected to next-generation sequencing(NGS). De novo assembly and reference-based assembly were completed using CLC Genomics Workbench v24.0. The dominant GII.17 contigs were further analyzed for similarity and phylogeny with BioEdit and MEGA. Results A total of 36 sewage samples were collected during the monitoring, and next-generation sequencing and analysis were performed on the RT-PCR product, with 21 genotypes identified, comprising 9 GI genotypes(GI.1–GI.9), 11 GII genotypes(including GII.1–GII.4, GII.6–GII.9, GII.12, GII.13, and GII.17), and the GIX.1 genotype. GI.2 and GII.17 were the common dominant genotypes in the monitoring area, with GII. 17 having the highest detection frequency. Phylogenetic analysis reveals that GII. 17 sequences in the sewage from the three cities have multiple distinct branching lineages and confirmed the co-circulation of multiple lineages in Shandong Province, China. Conclusion It is found through the study that the noroviruses in sewage in Shandong Province, China has high genetic diversity, with multiple genotypes co-circulating in the population, and variations were observed among monitoring areas in dominant genotype composition and epidemiological dynamics. The results further confirm that environmental surveillance serves as an effective tool for investigating the regional epidemiology of noroviruses in human populations.
[1]Ahmed SM, Hall AJ, Robinson AE, et al. Global prevalence of norovirus in cases of gastroenteritis:a systematic review and meta-analysis[J]. Lancet Infect Dis, 2014, 14(8):725-730. DOI:10. 1016/S1473-3099(14)70767-4.
[2]Lu J, Peng J, Fang L, et al. Capturing noroviruses circulating in the population:sewage surveillance in Guangdong, China(2013–2018)[J]. Water Res,2021, 196:116990. DOI:10. 1016/j.watres. 2021. 116990.
[3]Fumian TM, Fioretti JM, Lun JH, et al. Detection of norovirus epidemic genotypes in raw sewage using next generation sequencing[J]. Environ Int, 2019, 123:282-291. DOI:10. 1016/j. envint. 2018. 11. 054.
[4]宋杰，董少忠.人诺如病毒最新研究进展[J].病毒学报，2017, 33(5):785-790. DOI:10. 13242/j. cnki.bingduxuebao. 003209.
[5]廖颖茵，薛亮，高珺珊，等.诺如病毒分子结构特征及其衣壳蛋白功能研究进展[J].病毒学报，2021, 37(2):459-464. DOI:10. 13242/j. cnki.bingduxuebao. 003891.
[6]Chhabra P, de Graaf M, Parra GI, et al. Updated classification of norovirus genogroups and genotypes[J].J Gen Virol, 2019, 100(10):1393-1406. DOI:10. 1099/jgv. 0. 001318.
[7]Doh H, Lee C, Kim NY, et al. Genomic diversity and comparative phylogenomic analysis of genus Norovirus[J]. Sci Rep, 2025, 15:5412. DOI:10. 1038/s41598-025-87719-9.
[8]van Beek J, Ambert-Balay K, Botteldoorn N, et al.Indications for worldwide increased norovirus activity associated with emergence of a new variant of genotype II. 4, late 2012[J]. Euro Surveill,2013, 18(1):20345. DOI:10. 2807/ese. 18. 01. 20345-en.
[9]Wang S, Xu M, Lin X, et al. Detection of human noroviruses in sewage by next generation sequencing in Shandong Province, 2019–2021[J]. Virol J, 2025, 22(1):18. DOI:10. 1186/s12985-025-02638-5.
[10]Chan MCW, Lee N, Hung TN, et al. Rapid emergence and predominance of a broadly recognizing and fast-evolving norovirus GII. 17 variant in late 2014[J]. Nat Commun, 2015, 6:10061. DOI:10. 1038/ncomms10061.
[11]陶璐秋，王璇，余艳，等. 2019—2022年南京市急性胃肠炎暴发疫情中诺如病毒分子流行病学分析[J].病毒学报，2023, 39(5):1272-1284. DOI:10. 13242/j.cnki. bingduxuebao. 004393.
[12]Tao Z, Xu M, Lin X, et al. Environmental surveillance of genogroup I and II noroviruses in Shandong Province,China in 2013[J]. Sci Rep, 2015,5(1):17444. DOI:10. 1038/srep17444.
[13]王培，陶泽新，王素婷，等.环境污水中诺如、轮状、星状病毒的检测和分子流行病学研究[J].病毒学报，2016, 32(4):478-483. DOI:10. 13242/j. cnki.bingduxuebao. 002989.
[14]Kazama S, Miura T, Masago Y, et al. Environmental surveillance of norovirus genogroups I and II for sensitive detection of epidemic variants[J]. Appl Environ Microbiol, 2017, 83(9):e03406-e03416.DOI:10. 1128/aem. 03406-16.
[15]Lin X, Zou R, Liu Y, et al. Continuous detection of norovirus and astrovirus in wastewater in a coastal city of China in 2014–2016[J]. Lett Appl Microbiol, 2021,73(4):418-425. DOI:10. 1111/lam. 13530.
[16]Cao R, Ma X, Pan M. Molecular characteristics of norovirus in sporadic and outbreak cases of acute gastroenteritis and in sewage in Sichuan, China[J].Virol J, 2022, 19(1):180. DOI:10. 1186/s12985-022-01897-w.
[17]Ueki Y, Sano D, Watanabe T, et al. Norovirus pathway in water environment estimated by genetic analysis of strains from patients of gastroenteritis,sewage, treated wastewater, river water and oysters[J]. Water Res, 2005, 39(18):4271-4280. DOI:10. 1016/j. watres. 2005. 06. 035.
[18]Wang L, Ji L, Li H, et al. Early evolution and transmission of GII. P16-GII. 2 norovirus in China[J].G3(Bethesda), 2022, 12(11):jkac250. DOI:10. 1093/g3journal/jkac250
[19]Zhang Z, Liu D, Li S, et al. Imported human norovirus in travelers, Shanghai port, China 2018:an epidemiological and whole genome sequencing study[J]. Travel Med Infect Dis, 2021, 43:102140. DOI:10. 1016/j. tmaid. 2021. 102140.
[20]Chan MCW, Hu Y, Chen H, et al. Global spread of norovirus GII. 17 Kawasaki 308, 2014–2016[J].Emerg Infect Dis, 2017, 23(8):1350-1354. DOI:10. 3201/eid2308. 161138.
[21]Lu J, Sun L, Fang L, et al. Gastroenteritis outbreaks caused by norovirus GII. 17, Guangdong Province,China, 2014-2015[J]. Emerg Infect Dis, 2015, 21(7):1240-1242. DOI:10. 3201/eid2107. 150226.
[22]Ao Y, Wang J, Ling H, et al. Norovirus GII. P16/GII. 2–associated gastroenteritis, China, 2016[J].Emerg Infect Dis, 2017, 23(7):1172-1175. DOI:10. 3201/eid2307. 170034.
基本信息:
中图分类号:R373
引用信息:
[1]徐敏,林小娟,纪峰,等.山东省污水中诺如病毒的遗传多样性及GII.17型系统进化分析[J].病毒学报().
基金信息:
泰山学者青年专家计划项目(项目号:tsqn202103187),题目:基于同一健康理念和下一代测序技术新型病毒的发现、溯源和进化动态研究; 公共卫生人才培养支持项目
2026-07-09
2026-07-09
2026-07-09