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目的 本研究系统比较五株临床新分离的猪流行性腹泻病毒(Porcine epidemic diarrhea, PEDV)G2c亚型毒株的免疫原性。方法 将新分离PEDV毒株GX2311、QD2306、JX2307、NT2023和WF2023分别于Vero细胞中传代至第5代，测定S基因全长序列并进行遗传进化分析，通过生长曲线分析各毒株体外复制动力学，并分别制备灭活疫苗进行小鼠免疫，评估各毒株的免疫原性。结果 结果显示，新分离五株病毒均属于PEDV G2c亚型；生长动力学测定发现QD2306具有最高的增殖滴度，在感染后36 h，病毒滴度约为104.5 TCID50/mL；统一调整病毒滴度至103.5 TCID50/mL，经β-丙内酯灭活后，与佐剂ISA 201充分乳化制备灭活疫苗；对免疫后小鼠血清进行抗体测定，发现五株灭活疫苗均能显著诱导小鼠产生病毒特异性IgG抗体，其中QD2306株灭活疫苗诱导的中和抗体水平最高，其对同源毒株的中和效价为1:32，对G2b亚型毒株的中和效价为1:24。结论 上述结果提示，新分离的五株G2c亚型PEDV毒株中，QD2306株具有更优的免疫原性，可作为候选疫苗株进一步开展免疫保护与交叉保护研究。
Abstract：Objective To systematically compare the immunogenicity of five newly isolated porcine epidemic diarrhea virus(PEDV) strains belonging to the G2c subtypes. Methods Clinical PEDV isolates GX2311, QD2306, JX2307, NT2023, and WF2023 were passaged to the fifth passage in Vero cells. Full-length spike(S) genes were determined and subjected to phylogenetic analysis. In vitro replication kinetics were evaluated by multistep growth curves. Each strain was inactivated and formulated into an inactivated vaccine preparation, and immunogenicity was evaluated in mice.Results All five isolates clustered within the PEDV G2c lineage. Among them, QD2306 exhibited the highest replication capacity, reaching a peak titer of approximately of 104.5 TCID50 mL-1 at 36 h post-infection. Viral stocks were standardized to 103.5 TCID50 mL-1, inactivated with β-propiolactone, and emulsified with ISA 201 adjuvant to prepare inactivated vaccines. Serological analyses demonstrated that all five inactivated vaccines elicited robust virus-specific IgG responses in mice. Notably, the inactivated vaccine derived from QD2306 induced the highest neutralizing antibody titers, with a homologous neutralization titer of 1:32 and a heterologous neutralization titer of 1:24 against G2b strain. Conclusion Collectively, these results indicate that among the five newly isolated PEDV G2c strains, QD2306 possesses superior immunogenicity and represents a promising candidate for further evaluation in homologous and heterologous protection studies.
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基本信息:
DOI：10.13242/j.cnki.bingduxuebao.250308
中图分类号:S852.651
引用信息:
[1]杨孟迪,郭容利,莫嘉文,等.多株猪流行性腹泻病毒G2c毒株的分离与免疫原性比较[J].病毒学报,2026,42(03):996-1006.DOI:10.13242/j.cnki.bingduxuebao.250308.
基金信息:
国家自然科学基金（项目号：32373030）,题目：PEDV感染肠道杯状细胞下调黏蛋白Muc2的分子机制; 江苏省农业科技自主创新资金（项目号：CX(24)3071）,题目：高效刺激黏膜免疫应答防控PED的无抗乳酸菌制剂的研制; 江苏省基础研究计划（项目号：BK20230077）,题目：PEDV纤突蛋白S2'位点调控PARP9/DTX3L轴激活干扰素表达的分子机制~~
2025-09-28
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