病毒学报

本文报道了AciNPV-DNA的纯化及特性,证明纯化DNA具有典型紫外吸收光谱,在Sepharose 2B柱层析和超离心沉降分析中呈单一峰,Tm值为70.2℃,其(G+C)％=39.8％,增色效应为34％,限制性内切酶Pst Ⅰ,PvuⅡ,SaI Ⅰ,Eco R Ⅰ,BgI Ⅰ,XhoⅠ,Bgl Ⅱ酶切DNA,分别产生4,6,22,20,11,5,7个DNA片段,某些酶切电泳图谱中观察到亚分子片段,Bgl Ⅱ和EcoR Ⅰ双醇切DNA分子产生26个片段,Eco R Ⅰ酶切片段积加DNA分子量为56.55×10⁶道尔顿,约85.70千碱基对,电镜观察DNA分子有线状和环状,环状分子长约27.38μ,分子量约为53.94×10^?道尔顿,以Eco R Ⅰ对1981～1985年林间连续复制回收的AciNPV的DNA同源性进行检测,结果无变化,AciNPV攻毒枣尺蠖幼虫所得枣尺蠖NPV,其DNA的Bgl Ⅱ图谱与AciNPV-DNA的Bgl Ⅰ酶谱有明显的差异。

In this paper, the purification and characteristics of DNA of nuclear polyhedrosis virus of the poplar looper, Apocheima cinerarius are reported. The purified AciNPV-DNA presents the characteristic ultraviolet absorption spectrum of DNA. A single peak was observed by analysing the Sepharose 2B gel column chromatography and ultracentrifuge sedimentation. The melting temperature of AciNPV-DNA was 70.2℃, therefore, its content of (G + C) is about 39.8%, and the thermal hyperchromic effect is 34%. The restriction endonucleases assay revealed that Pst Ⅰ, Pvu Ⅱ , Sal Ⅰ, EcoR Ⅰ, Bgl Ⅰ, Xho Ⅰ, Bgl Ⅱ, divided the AciNPV-DNA into 4, 6, 22, 20, 11, 5, 7 bands respectively. The "submolar fragment" was observed in some patterns. Double-digestion with Bgl Ⅱ+ EcoR Ⅰ, divided the AciNPV-DNA into 26 fragments. The size of AciNPV-DNA EcoR Ⅰfragments added up to be about 56.55 Mdal(85.70kb). The release of AciNPV-DNA from the nuc-leocapsids of AciNPV was observed. Purified AciNPV-DNA observed by electron microscopy had a mean contour length of about 27.38μ, or about 53.94 Mdal. In an attempt to detect genetic variation of AciNPV as a biological control agent in the field from the year 1981 to 1985 using EcoR Ⅰ, no difference in restriction patterns was observed. Using AciNPV to infect the larvae of looper Sucra jujuba, a strain NPV was isolated from the diseased larvae of Sucra jujuba. The Bgl Ⅱ pattern of Sucra jujuba NPV DNA was different from the AciNPV-DNA Bgl Ⅱ.