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新疆幅员辽阔,是我国最大的省份,同时也是多种人兽共患病的自然疫源地。作为很多人兽共患病毒的自然宿主,蝙蝠在新疆的地理分布、种类和数量等背景资料目前尚不清楚,其携带的病毒多样性数据更为欠缺。为了掌握新疆伊犁州蝙蝠携带病毒的病原本底情况,本研究在2个地点采集了122只蝙蝠样品。病毒宏基因组学分析揭示这些蝙蝠携带18个科的病毒,包括噬菌体、昆虫病毒、脊椎动物病毒等。根据病毒宏基因组学结果,挑选冠状病毒、圆环病毒、星状病毒、副肠孤病毒等进行特异性PCR检测,发现其阳性率为1.3%～10.5%,其中星状病毒具有丰富的遗传多样性,部分和猪源星状病毒具有很高的同源性,提示可能发生跨种传播;而副肠孤病毒则是首次从蝙蝠中发现。本研究首次开展了新疆伊犁州蝙蝠病毒组研究,为了解新疆伊犁州蝙蝠携带病毒的多样性和本底情况提供了重要的基础数据。
Abstract：As the largest provincial region in China,Xinjiang is a natural focus of epidemics of several zoonoses.Bats are considered to be important natural reservoirs of many zoonotic viruses,but their geographical distribution,species and numbers in Xinjiang are not known,which makes identification of the virus diversity harbored by bats in this area extremely difficult.To understand the background of bat viruses in Xinjiang,bat samples from 122 individuals were collected from two locations.Viral metagenomic analyses revealed that these bats carried 18 families of viruses,including phage,insect viruses,and vertebrate viruses.According to the results of viral metagenomics,a specific polymerase chain reaction(PCR)was undertaken for coronavirus,circovirus,astrovirus and parechovirus.As a result,their positive rates ranged from 1.3% to 10.5%.In particular,astroviruses exhibited rich genetic diversity with some showing high identity with porcine astroviruses(suggesting possible cross-species transmission),whereas parechovirus was discovered for the first time in bats.We profiled the viromic composition harbored by bats in Xinjiang,and these fundamental data can help understanding of the diversity and background of the virus carried by bats this region.
[1]冯崇慧,白旭华,刘宏斌,李凡,顾媛.新疆准噶尔盆地南缘地区新疆出血热病毒自然疫源地的发现[J].疾病预防控制通报,1991,6(1):52-55.
[2]郭刚,徐军,黄林,盛金良,阿布都扎伊尔.新疆地区鼠类汉坦病毒核酸检测和基因分型[J].中国媒介生物学及控制杂志,2013,24(2):144-146.
[3]滕云峰,张鸿猷,谢杏初.新疆鼠疫自然疫源地[J].疾病预防控制通报,1994,9(3):35-41.
[4]谢杏初,于心,张挺秀,蒋果义.新疆天山、阿尔泰山山地森林脑炎自然疫源地考察报告[J].疾病预防控制通报,1991,6(3):109-114.
[5]Beer J R,Richards A G.Hibernation of the Big Brown Bat[J].J Mammal,1956,37(1):31-41.
[6]Moratelli R,Calisher C H.Bats and zoonotic viruses:can we confidently link bats with emergingdeadly viruses?[J].Mem Inst Oswaldo Cruz,2015,110(1):1-22.
[7]Feng J,Sun K P,Zhang Z Z,Xu L J,Liu Y.Cryptic diversity in Chinese rhinolophids and hipposiderids(Chiroptera:Rhinolophidae and Hipposideridae):mammalia[J].Mammalia,2009,73(2):135-141.
[8]何彪.蝙蝠病毒组学及其新病毒的发现与鉴定[D].中国人民解放军军事医学科学院,2014.
[9]杨凡力.中缅边境地区蝙蝠新型病毒的检测与鉴定[D].吉林大学,2013.
[10]Ge X Y,Li J L,Yang X L,Chmura A A,Zhu G,Epstein J H,Mazet J K,Hu B,Zhang W,Peng C,Zhang Y J,Luo C M,Tan B,Wang N,Zhu Y,Grameri G,Zhang S Y,Wang L F,Daszak P,Shi Z L.Isolation and characterization of a bat SARS-like coronavirus that uses the ACE2receptor[J].Nature,2013,503(7477):535-538.
[11]Zhou L,Sun Y,Lan T,Wu R T,Chen J W,Wu Z X,Xie Q M,Zhang X B,Ma J Y.Retrospective detection and phylogenetic analysis of swine acute diarrhea syndrome coronavirus in pigs in southern China[J].Transbound Emerg Dis,2018.
[12]Ge X,Li Y,Yang X,Zhang H,Zhou P,Zhang Y,Shi Z.Metagenomic analysis of viruses from bat fecal samples reveals many novel viruses in insectivorous bats in china[J].J Virol,2012,86(8):4620-4630.
[13]He B,Li Z,Yang F,Zheng J,Feng Y,Guo H,Li Y,Wang Y,Su N,Zhang F,Fan Q,Tu C.Virome profiling of bats from Myanmar by metagenomic analysis of tissue samples reveals more novel Mammalian viruses[J/OL].PLoS One,2013,8(4):e61950.
[14]Wu Z,Ren X,Yang L,Hu Y,Yang J,He G,Zhang J,Dong J,Sun L,Du J,Liu L,Xue Y,Wang J,Yang F,Zhang S,Jin Q.Virome analysis for identification of novel mammalian viruses in bat species from Chinese provinces[J].J Virol,2012,86(20):10999-11012.
[15]Drexler J F,Geipel A,K9nig A,Corman V M,van Riel D,Leijten L M,Bremer C M,Rasche A,Cottontail V M,Maganga G D,Schlegel M,Müller M A,Adam A,Klose S M,Carneiro A J,St9cker A,Franke C R,Gloza-Rausch F,Geyer J,Annan A,Adu-Sarkodie Y,Oppong S,Binger T, Vallo P,Tschapka M,Ulrich R G,Gerlich W H,Leroy E,Kuiken T,Glebe D,Drosten C.Bats carry pathogenic hepadnaviruses antigenically related to hepatitis B virus and capable of infecting human hepatocytes[J].Proc Natl Acad Sci U S A,2013,110(40):16151-16156.
[16]He B,Fan Q,Yang F,Hu T,Qiu W,Feng Y,Li Z,Li Y,Zhang F,Guo H,Zou X,Tu C.Hepatitis virus in long-fingered bats,Myanmar[J].Emerg Infect Dis,2013,19(4):638-640.
[17]Yang L,Wu J,Hu T,Qin S,Deng B,Liu J,Zhang F,He B,Tu C.Genetic diversity of bat orthohepadnaviruses in China and a proposed new nomenclature[J].Infect Genet Evol,2018,63:135-143.
[18]Corman V M,Eckerle I,Memish Z A,Liljander A M,Dijkman R,Jonsdottir H,Juma Ngeiywa K J,Kamau E,Younan M,Al Masri M,Assiri A,Gluecks I,Musa B E,Meyer B,Müller MA,Hilali M,Bornstein S,Wernery U,Thiel V,Jores J,Drexler J F,Drosten C.Link of a ubiquitous human coronavirus to dromedary camels[J].Proc Natl Acad Sci U S A,2016,113(35):9864-9869.
[19]Fischer T K,Midgley S,Dalgaard C,Nielsen A Y.Human Parechovirus Infection,Denmark[J].Emerg Infect Dis,2014,20(1):83-87.
[20]Joffret M L,Bouchier C,Grandadam M,Zeller H,Maufrais C,Bourhy H,Despres P,Delpeyroux F,Dacheux L.Genomic characterization of Sebokele virus 1(SEBV1)reveals a new candidate species among the genus Parechovirus[J].J Gen Virol,2013,94(Pt 7):1547-1553.
[21]Smits S L,Raj V S,Oduber M D,Schapendonk C M,Bodewes R,Provacia L,Stittelaar K J,Osterhaus A D,Haagmans B L.Metagenomic analysis of the ferret fecal viral flora[J/OL]. PLoS One, 2013, 8(8):e71595.
[22]Mitake H,Fujii Y,Nagai M,Ito N,Okadera K,Okada K,Nakagawa K,Kishimoto M,Mizutani T,Okazaki K,Sakoda Y,Takada A,Sugiyama M.Isolation of a sp.nov.Ljungan virus from wild birds in Japan[J].J Gen Virol,2016,97(8):1818-1822.
[23]Zhu A,Jiang T,Hu T,Mi S,Zhao Z,Zhang F,Feng J,Fan Q,He B,Tu C.Molecular characterization of a novel bat-associated circovirus with a poly-T tract in the 3′intergenic region[J].Virus Res,2018,250:95-103.
基本信息:
DOI：10.13242/j.cnki.bingduxuebao.003476
中图分类号:S852.65
引用信息:
[1]张畅,燕超,赵梓含,等.新疆伊犁州蝙蝠的病毒宏基因组学研究[J].病毒学报,2018,34(06):896-903.DOI:10.13242/j.cnki.bingduxuebao.003476.
基金信息:
NSFC-新疆联合基金(项目号:U1503283),题目:新疆重要自然宿主和媒介昆虫携带人兽共患病毒的病原生态学与分子流行病学研究~~
2018-11-28
2018-11-28
2018-11-28