| 1,714 | 16 | 231 |
| 下载次数 | 被引频次 | 阅读次数 |
近年来,以CRISPR/Cas9为代表的基因编辑技术发展迅速,与这一技术相关的基础和应用研究已成为全球生命科学各个领域的研究热点。在病毒学研究中,病毒基因组改造技术一直以来都是研究病毒基因功能、致病机制以及疫苗研发的重要技术手段。本文主要从传统的病毒基因组改造技术,CRISPR/Cas9基因编辑系统的起源、原理、发展以及在病毒基因编辑方面的应用和进展等方面进行阐述,重点介绍该系统在病毒学研究中已取得的最新进展和成果,并就CRISPR/Cas9基因编辑技术现存的问题及应用前景进行简要论述。
Abstract:In recent years,the CRISPR/Cas9-based gene-editing system has been developed and applied widely. Modification of the viral genome is very important for revealing gene functions,pathogenesis and vaccine development. Herein,we provide an overview of traditional technology for modification of the viral genome, origin and functional principles of the CRISPR/Cas9 system, and its latest applications and achievements in virology. The problems and application prospects of the CRISPR/Cas9-based gene-editing system are also discussed briefly.
[1]He T C,Zhou S,da Costa L T,Yu J,Kinzler K W,Vogelstein B.A simplified system for generating recombinant adenoviruses[J].Proc Natl Acad Sci U SA,1998,95(5):2509-2514.
[2]Wong H H,Jiang G,Gangeswaran R,Wang P,Wang J,Yuan M,Wang H,Bhakta V,Müller H,Lemoine NR,Wang Y.Modifification of the early gene enhancerpromoter improves the oncolytic potency of adenovirus11[J].Mol Ther,2012,20(2):306-316.
[3]Ruzsics Z,Lemnitzer F,Thirion C.Engineering adenovirus genome by bacterial artifificial chromosome(BAC)technology[J].Methods Mol Biol,2014,1089,143-158.
[4]Hokanson C A,Dora E,Donahue B A,Rivkin M,Finer M,Mendez M J.Hybrid yeast-bacteria cloning system used to capture and modify adenoviral and nonviral genomes[J].Hum Gene Ther,2003,14(4):329-339.
[5]Post L E,Roizman B.A generalized technique for deletion of specifific genes in large genomes:alpha gene22 of herpes simplex virus 1 is not essential for growth[J].Cell,1981,25(1):227-232.
[6]Almazán F,González J M,Pénzes Z,Izeta A,Calvo E,Plana-Durán J,Enjuanes L.Engineering the largest RNA virus genome as an infectious bacterial artifificial chromosome[J].Proc Natl Acad Sci U S A,2000,97(10):5516-5521.
[7]Domi A,Moss B.Cloning the vaccinia virus genome as a bacterial artifificial chromosome in Escherichia coli and recovery of infectious virus in mammalian cells[J].Proc Natl Acad Sci U S A,2002,99(19):12415-12420.
[8]Luckow V A,Lee S C,Barry G F,Olins P O.Effificient generation of infectious recombinant baculoviruses by sitespecifific transposon-mediated insertion of foreign genes into a baculovirus genome propagated in Escherichia coli[J].J Virol,1993,67(8):4566-4579.
[9]Messerle M,Crnkovic I,Hammerschmidt W,Ziegler H,Koszinowski U H.Cloning and mutagenesis of a herpesvirus genome as an infectious bacterial artifificial chromosome[J].Proc Natl Acad Sci U S A,1997,94(26):14759-14763.
[10]O'connor M,Peifer M,Bender W.Construction of large DNA segments in Escherichia coli[J].Science,1989,244(4910):1307-1312.
[11]Tanaka M,Kagawa H,Yamanashi Y,Sata T,Kawaguchi Y.Construction of an excisable bacterial artifificial chromosome containing a full-length infectious clone of herpes simplex virus type 1:viruses reconstituted from the clone exhibit wild-type properties in vitro and in vivo[J].J Virol,2003,77(2):1382-1391.
[12]Zhang Y,Buchholz F,Muyrers J P,Stewart A F.Anew logic for DNA engineering using recombination in Escherichia coli[J].Nat Genet,1998,20(2):123-128.
[13]Bakota L,Brandt R,Heinisch J J.Triple mammalian/yeast/bacterial shuttle vectors for single and combined Lentivirus-and Sindbis virus-mediated infections of neurons[J].Mol Genet Genomics,2012,287 (4):313-324
[14]Ishino Y,Shinagawa H,Makino K,Amemura M,Nakata A.Nucleotide sequence of the iap gene,responsible for alkaline phosphatase isozyme conversion in Escherichia coli,and identification of the gene product[J].J Bacteriol,1987,169(12):5429-5433.
[15]Jansen R,Embden J D,Gaastra W,Schouls L M.Identification of genes that are associated with DNArepeats in prokaryotes[J].Mol Microbiol,2002,43(6):1565-1575.
[16]Mojica F J,Diez-Villasenor C,Garcia-Martinez J,Soria E.Intervening sequences of regularly spaced prokaryotic repeats derive from foreign genetic elements[J].J Mol Evol,2005,60(2):174-182.
[17]Barrangou R,Fremaux C,Deveau H,Richards M,Boyaval P,Moineau S,Romero D A,Horvath P.CRISPR provides acquired resistance against viruses in prokaryotes[J].Science,2007,315(5819):1709-1712.
[18]Makarova K S,Wolf Y I,Alkhnbashi O S,Costa F,Shah S A,Saunders S J,Barrangou R,Brouns S J,Charpentier E,Haft D H,Horvath P,Moineau S,Mojica F J,Terns R M,Terns M P,White M F,Yakunin A F,Garrett R A,van der Oost J,Backofen R,Koonin E V.An updated evolutionary classification of CRISPR-Cas systems[J].Nat Rev Microbiol,2015,13(11):722-736.
[19]Deltcheva E,Chylinski K,Sharma C M,Gonzales K,Chao Y,Pirzada Z A,Eckert M R,Vogel J,Charpentier E.CRISPR RNA maturation by transencoded small RNA and host factor RNase III[J].Nature,2011,471(7340):602-607.
[20]Deveau H,Garneau J E,Moineau S.CRISPR/Cas system and its role in phage-bacteria interactions[J].Annu Rev Microbiol,2010,64:475-493.
[21]Gasiunas G,Barrangou R,Horvath P,Siksnys V.Cas9-cr RNA ribonucleoprotein complex mediates specifific DNA cleavage for adaptive immunity in bacteria[J/OL].Proc Natl Acad Sci U S A,2012,109(39):E2579-2586.
[22]Jinek M,Chylinski K,Fonfara I,Hauer M,Doudna JA,Charpentier E.A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity[J].Science,2012,337(6096):816-821.
[23]Cong L,Ran F A,Cox D,Lin S,Barretto R,Habib N,Hsu P D,Wu X,Jiang W,Marraffini L A,Zhang F.Multiplex genome engineering using CRISPR/Cas systems[J].Science,2013,339(6121):819-823.
[24]Wang H,Yang H,Shivalila C S,Dawlaty M M,Cheng A W,Zhang F,Jaenisch R.One-step generation of mice carrying mutations in multiple genes by CRISPR/Cas-mediated genome engineering[J].Cell,2013,153(4):910-918.
[25]Yang H,Wang H,Shivalila C S,Cheng A W,Shi L,Jaenisch R.One-step generation of mice carrying reporter and conditional alleles by CRISPR/Casmediated genome engineering[J].Cell,2013,154(6):1370-1379.
[26]Niu Y,Shen B,Cui Y,Chen Y,Wang J,Wang L,Kang Y,Zhao X,Si W,Li W,Xiang A P,Zhou J,Guo X,Bi Y,Si C,Hu B,Dong G,Wang H,Zhou Z,Li T,Tan T,Pu X,Wang F,Ji S,Zhou Q,Huang X,Ji W,Sha J.Generation of gene-modified cynomolgus monkey via Cas9/RNA-mediated gene targeting in one-cell embryos[J].Cell,2014,156(4):836-843.
[27]Kou Z,Wu Q,Kou X,Yin C,Wang H,Zuo Z,Zhuo Y,Chen A,Gao S,Wang X.CRISPR/Cas9-mediated genome engineering of the ferret[J].Cell Res,2015,25(12):1372-1375.
[28]Liao H K,Li M,Martinez Martinez L,Izpisua Belmonte J C.Stem cell,CRISPR and HIV[J].Cell Cycle,2015,14(13):1991-1992.
[29]Liao H K,Gu Y,Diaz A,Marlett J,Takahashi Y,Li M,Suzuki K,Xu R,Hishida T,Chang C J,Esteban CR,Young J,Izpisua Belmonte J C.Use of the CRISPR/Cas9 system as an intracellular defense against HIV-1infection in human cells[J].Nat Commun,2015,6:6413.
[30]Li J F,Zhang D,Sheen J.Targeted plant genome editing via the CRISPR/Cas9 technology[J].Methods Mol Biol,2015,1284:239-255.
[31]Ebina H,Misawa N,Kanemura Y,Koyanagi Y.Harnessing the CRISPR/Cas9 system to disrupt latent HIV-1 provirus[J].Sci Rep,2013,3:2510.
[32]Hu W,Kaminski R,Yang F,Zhang Y,Cosentino L,Li F,Luo B,Alvarez-Carbonell D,Garcia-Mesa Y,Karn J,Mo X,Khalili K.RNA-directed gene editing specifically eradicates latent and prevents new HIV-1infection[J].Proc Natl Acad Sci U S A,2014,111(31):11461-11466.
[33]Liao H K,Gu Y,Diaz A,Marlett J,Takahashi Y,Li M,Suzuki K,Xu R,Hishida T,Chang C J,Esteban CR,Young J,Izpisua Belmonte J C.Use of the CRISPR/Cas9 system as an intracellular defense against HIV-1infection in human cells[J].Nat Commun,2015,6:6413.
[34]Lebbink R J,de Jong D C,Wolters F,Kruse E M,van Ham P M,Wiertz E J,Nijhuis M.A combinational CRISPR/Cas9 gene-editing approach can halt HIVreplication and prevent viral escape[J].Sci Rep,2017,7:41968.
[35]Wang Q,Liu S,Liu Z,Ke Z,Li C,Yu X,Chen S,Guo D.Genome scales creening identification of Sa Cas9/g RNAs for targeting HIV-1 provirus and suppression of HIV-1 infection[J].Virus Res,2018,250:21-30.
[36]Xiao Q,Guo D,Chen S.Application of CRISPR Cas9based gene editing in HIV-1 AIDS therapy[J].Front Cell Infect Microbiol,2019,9:69.
[37]Bi Y,Sun L,Gao D,Ding C,Li Z,Li Y,Cun W,Li Q.High-efficiency targeted editing of large viral genomes by RNA-guided nucleases[J/OL].PLo SPathog,2014,10(5):e1004090.
[38]Kennedy E M,Kornepati A V,Cullen B R.Targeting hepatitis B virus ccc DNA using CRISPR/Cas9[J].Antiviral Res,2015,123:188-192.
[39]Lin S R,Yang H C,Kuo Y T,Liu C J,Yang T Y,Sung K C,Lin Y Y,Wang H Y,Wang C C,Shen YC,Wu F Y,Kao J H,Chen D S,Chen P.The CRISPR/Cas9 system facilitates clearance of the intrahepatic HBV templates in vivo[J/OL].Mol Ther Nucleic Acids,2014,3(8):e186.
[40]Wang J,Xu Z W,Liu S,Zhang R Y,Ding S L,Xie XM,Long L,Chen X M,Zhuang H,Lu F M.Dual g RNAs guided CRISPR/Cas9 system inhibits hepatitis B virus replication[J].World J Gastroenterol,2015,21(32):9554-965.
[41]Zhen S,Hua L,Liu Y H,Gao L C,Fu J,Wan D Y,Dong L H,Song H F,Gao X.Harnessing the clustered regularly interspaced short palindromic repeat(CRISPR)/CRISPR-associated Cas9 system to disrupt the hepatitis B virus[J].Gene Ther,2015,22(5):404-412.
[42]Dong C,Qu L,Wang H,Wei L,Dong Y,Xiong S.Targeting hepatitis B virus ccc DNA by CRISPR/Cas9nuclease efficiently inhibits viral replication[J].Antiviral Res,2015,118:110-117.
[43]Ramanan V,Shlomai A,Cox D B,Schwartz R E,Michailidis E,Bhatta A,Scott D A,Zhang F,Rice CM,Bhatia S N.CRISPR/Cas9 cleavage of viral DNAefficiently suppresses hepatitis B virus[J].Sci Rep,2015,5:10833.
[44]Sakuma T,Masaki K,Abe-Chayama H,Mochida K,Yamamoto T,Chayama K.Highly multiplexed CRISPR-Cas9-nuclease and Cas9-nickase vectors for inactivation of hepatitis B virus[J].Genes Cells,2016,21(11):1253-1262.
[45]Hu Z,Yu L,Zhu D,Ding W,Wang X,Zhang C,Wang L,Jiang X,Shen H,He D,Li K,Xi L,Ma D,Wang H.Disruption of HPV16-E7 by CRISPR/Cas system induces apoptosis and growth inhibition in HPV16 positive human cervical cancer cells[J].Biomed Res Int,2014,2014:612823.DOI:10.1155/2014/612823
[46]Zhen S,Hua L,Takahashi Y,Ding W,Wang X,Zhang C,Wang L,Jiang X,Shen H,He D,Li K,Xi L,Ma D,Wang H.In vitro and in vivo growth suppression of human papillomavirus 16-positive cervical cancer cells by CRISPR/Cas9[J].Biochem Biophys Res Commun,2014,450(4):1422-1426.
[47]van Diemen F R,Lebbink R J.CRISPR/Cas9,a powerful tool to target human herpesviruses[J].Cell Microbiol,2017,19(2).DOI:10.1111/cmi.12694
[48]Suenaga T,Kohyama M,Hirayasu K,Arase H.Engineering large viral DNA genomes using the CRISPR-Cas9 system[J].Microbiol Immunol,2014,58(9):513-522.
[49]van Diemen F R,Kruse E M,Hooykaas M J,Bruggeling C E,Schürch A C,van Ham P M,Imhof SM,Nijhuis M,Wiertz E J,Lebbink R J.CRISPR/Cas9-mediated genome editing of herpesviruses limits productive and latent infections[J/OL].PLo S Pathog,2016,12(6):e1005701.
[50]Roehm P C,Shekarabi M,Wollebo H S,Bellizzi A,He L,Salkind J,Khalili K.Inhibition of HSV-1replication by gene editing strategy[J].Sci Rep,2016,6:23146.
[51]Wang D,Wang X W,Peng X C,Xiang Y,Song S B,Wang Y Y,Chen L,Xin V W,Lyu Y N,Ji J,Ma ZW,Li C B,Xin H W.CRISPR/Cas9 genome editing technology significantly accelerated herpes simplex virus research[J].Cancer Gene Ther,2018,25(5-6):93-105.
[52]Wang J,Quake S R.RNA-guided endonuclease provides a therapeutic strategy to cure latent herpesviridae infection[J].Proc Natl Acad Sci U S A,2014,111(36):13157-13162.
[53]Yuen K S,Chan C P,Kok K H,Jin D Y.Mutagenesis and genome engineering of Epstein-Barr virus in cultured human cells by CRISPR/Cas9[J].Methods Mol Biol,2017,1498:23-31.
[54]Yuen K S,Wang Z M,Wong N M,Zhang Z Q,Cheng T F,Lui W Y,Chan C P,Jin D Y.Suppression of Epstein-Barr virus DNA load in latently infected nasopharyngeal carcinoma cells by CRISPR/Cas9[J].Virus Res,2018,244:296-303.
[55]Gergen J,Coulon F,Creneguy A,Elain-Duret N,Gutierrez A,Pinkenburg O,Verhoeyen E,Anegon I,Nguyen T H,Halary F A,Haspot F.Multiplex CRISPR/Cas9 system impairs HCMV replication by excising an essential viral gene[J/OL].PLo S One,2018,13(2):e0192602.
[56]Tso F Y,West J T,Wood C.Reduction of Kaposi's sarcoma-associated herpesvirus latency using CRISPR-Cas9 to edit the latency-associated nuclear antigen gene[J/OL].J Virol,2019,93(7):e02183-18.
[57]Xu A,Qin C,Lang Y,Wang M,Lin M,Li C,Zhang R,Tang J.A simple and rapid approach to manipulate pseudorabies virus genome by CRISPR/Cas9 system[J].Biotechnol Lett,2015,37(6),1265-1272.
[58]Liang X,Sun L,Yu T,Pan Y,Wang D,Hu X,Fu Z,He Q,Cao G.A CRISPR/Cas9 and Cre/Lox system-based express vaccine development strategy against re-emerging Pseudorabies virus[J].Sci Rep,2016,6:19176.
[59]Ren L Z,Peng Z Y,Ouyang T,Liu X H,Chen X R,Ye L,Fan J W,Ouyang H S,Pang D X,Bai J Y.Subculturing cells have no effect on CRISPR/Cas9-mediated cleavage of UL30 gene in pseudorabies virus[J].Animal Model Exp Med,2018,1(1):74-77.
[60]Peng Z,Ouyang T,Pang D,Ma T,Chen X,Guo N,Chen F,Yuan L,Ouyang H,Ren L.Pseudorabies virus can escape from CRISPR-Cas9-mediated inhibition[J].Virus Res,2016,223:197-205.
[61]于之清,童武,郑浩,李国新,高飞,王涛,梁超,叶超,武吉强,黄勤峰,童光志.使用CRISPR/Cas9技术构建新型重组伪狂犬病毒疫苗的初步研究[J].中国动物传染病学报,2017,25(4):6-12.
[62]Yao,Y,Bassett,A,Nair V.Targeted editing of avian herpesvirus vaccine vector using CRISPR/Cas9nucleases[J].J Vaccine Technol,2016,1:1-7.
[63]Zou Z,Huang K,Wei Y,Chen H,Liu Z,Jin M.Construction of a highly efficient CRISPR/Cas9-mediated duck enteritis virus-based vaccineagainst H5N1avian influenza virus and duck tembusu virus infection[J].Sci Rep,2017,7(1):1478.
[64]Tang N,Zhang Y,Pedrera M,Chang P,Baigent S,Moffat K,Shen Z,Nair V,Yao Y.A simple and rapid approach to develop recombinant avian herpesvirus vectored vaccines using CRISPR/Cas9 system[J].Vaccine,2018,36(5):716-722.
[65]Zhang Y,Tang N,Sadigh Y,Baigent S,Shen Z,Nair V,Yao Y.Application of CRISPR/Cas9 gene editing system on MDV-1 genome for the study of gene function[J/OL].Viruses,2018,10(6),E279.
[66]Zhang Y,Luo J,Tang N,Teng M,Reddy V R A P,Moffat K,Shen Z,Nair V,Yao Y.Targeted Editing of the pp38 gene in Marek's disease virus-transformed cell lines using CRISPR/Cas9 system[J/OL].Viruses,2019,11(5),E391.
[67]Yuan M,Gao X,Chard L S,Ali Z Ahmed J,Li Y,Liu P,Lemoine N R,Wang Y.A marker-free system for highly efficient construction of vaccinia virus vectors using CRISPR Cas9[J].Mol Ther Methods Clin Dev,2015,2:15035.
[68]Yuan M,Zhang W,Wang J,Al Yaghchi C,Ahmed J,Chard L,Lemoine N R,Wang Y.Efficiently editing the vaccinia virus genome by using the CRISPR-Cas9system[J].J Virol,2015,89(9):5176-5179.
[69]Chou Y Y,Krupp A,Kaynor C,Gaudin R,Ma M,Cahir-Mc Farland E,Kirchhausen T.Inhibition of JCPy V infection mediated by targeted viral genome editing using CRISPR/Cas9[J].Sci Rep,2016,6:36921.
[70]Pan Q,Wang J,Gao Y,Cui H,Liu C,Qi X,Zhang Y,Wang Y,Wang X.The natural large genomic deletion is unrelated to the increased virulence of the novel genotype fowl adenovirus 4 recently emerged in China[J/OL].Viruses,2018,10(9):E494.
[71]Borca M V,Holinka L G,Berggren K A,Gladue D P.CRISPR-Cas9,a tool to efficiently increase the development of recombinant African swine fever viruses[J].Sci Rep,2018,8(1):3154.
[72]Price A A,Sampson T R,Ratner H K,Grakoui A,Weiss D S.Cas9-mediated targeting of viral RNA in eukaryotic cells[J].Proc Natl Acad Sci U S A,2015,112(19):6164-6169.
基本信息:
DOI:10.13242/j.cnki.bingduxuebao.003765
中图分类号:Q78;Q939.4
引用信息:
[1]滕蔓,刘金玲,郑鹿平,等.CRISPR/Cas9基因编辑技术在病毒学研究中的应用及进展[J].病毒学报,2020,36(05):946-954.DOI:10.13242/j.cnki.bingduxuebao.003765.
基金信息:
国家自然科学基金(项目号:U1604232),题目:鸡马立克病病毒(MDV)遗传、变异与致病的分子机制;国家自然科学基金(项目号:31602050),题目:马立克氏病病毒(MDV)编码的miRNA抑癌基因的发现与鉴定; 中原千人计划-中原基础研究领军人才; 河南省重点研发与推广专项(科技攻关)(项目号:192102110072),题目:基于CRISPR基因编辑技术的鸡马立克病病毒MEQ癌蛋白抗体筛选与鉴定; 河南省农业科学院杰出青年科技基金(项目号:2019JQ01),题目:家禽疱疹病毒CRISPR/Cas9基因编辑技术平台的建立与应用~~
2019-08-31
2019
2019-11-13
2020-06-24
2020
1
2020-06-23
2020-06-23
2020-06-23