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为获得狂犬病病毒和犬细小病毒的二联疫苗,本研究运用分子生物学技术在狂犬病病毒基因组的G基因与L基因之间插入犬细小病毒VP2基因,通过反向遗传操作系统拯救获得了含犬细小病毒VP2基因的嵌合狂犬病病毒HEP-Flury(VP2),并研究了该病毒的生物学特性。结果表明,嵌合狂犬病病毒HEP-Flury(VP2)可以在BHK-21细胞上稳定繁殖,在传代10次后,外源基因VP2能高效表达;以HEP-Flury(VP2)免疫小鼠,可以诱导免疫小鼠产生抗狂犬病病毒和抗犬细小病毒的抗体。
Abstract：To obtain a bivalence vaccine against canine rabies virus and canine parvovirus,a chimeric rabies virus expressing canine parvovirus VP2 protein was generated by the technique of reverse genetics.It was shown that the chimeric virus designated as HEP-Flury(VP2) grew well on BHK-21 cells and the VP2 gene could still be stably expressed after ten passages on BHK-21 cells.Experiments on the mice immunized with the chimeric virus HEP-Flury(VP2) demonstrated that specific antibodies against rabies virus and canine parvovirus were induced in immunized mice after vaccination with the live chimeric virus.
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基本信息:
DOI：10.13242/j.cnki.bingduxuebao.002023
中图分类号:S852.655
引用信息:
[1]牛学锋,刘晓慧,孙招金,等.表达犬细小病毒VP2蛋白的嵌合狂犬病病毒生物学特性的研究[J].病毒学报,2009,25(05):362-367.DOI:10.13242/j.cnki.bingduxuebao.002023 .
基金信息:
国家“863”项目(2006AA10A204)
2009-09-15
2009-09-15