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本文报道了对分离自湖北地区的A型塞尼卡病毒(Senecavirus A,SVA)CH-HB-2017毒株进行鉴定和生物学特性研究。全基因序列分析显示,该分离株与国内毒株CH-HN-2017、CH-HNSL-2017、CH-FJ-2017和美国毒株USA-IA44952-2015、USA-IA44662-2015和USA-SD41901-2015的同源性高。该SVA可在猪肾传代细胞(IBRS-2)和猪睾丸细胞(Swine testis cells,ST)中复制增殖,感染后都能产生CPE,通过病毒一步生长曲线、蚀斑试验和定量PCR等试验表明,该毒株对IBRS-2细胞更易感,病毒滴度更高。本实验成功分离到1株SVA流行毒株,并研究了该毒株的生物学特性,为深入研究该病毒奠定基础。
Abstract：Identification and study of the biological characteristics of the CH-HB-2017 strain of senecavirus A(SVA)isolated from Hubei(China)were undertaken.Whole-gene sequencing showed that the isolated strain was highly homologous to some parts of Chinese and American strains:CH-HN-2017,CH-HNSL-2017,CH-FJ-2017,USA-IA44952-2015,USA-IA44662-2015 and USA-SD41901-2015.It could be replicated and propagated in a porcine kidney cell line(IBRS-2),porcine testis cells,and carbapenemase-producing Enterobacteriaceae were noted after infection.A single-step virus growth curve,plaque assay and quantitative polymerase chain reaction showed that SVA was more susceptible to IBRS-2 cells,and a higher virus titer in these cells was observed.We isolated an epidemic strain of SVA and studied its biological characteristics,thereby laying the foundation for further research of Senecavirus A.
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基本信息:
DOI：10.13242/j.cnki.bingduxuebao.003438
中图分类号:S852.65
引用信息:
[1]郭笑然,韩世充,张小丽,等.A型塞尼卡病毒的分离鉴定及生物学特性研究[J].病毒学报,2018,34(05):555-563.DOI:10.13242/j.cnki.bingduxuebao.003438.
基金信息:
国家自然科学基金项目(项目号:31672592),题目:仿生矿化优化口蹄疫病毒样颗粒耐热抗逆性研究; 中国农业科学院青年英才计划项目; 国家重点研发计划(项目号:2017YFD0500900),题目:牛羊疫苗新型佐剂及工艺研究~~
2018-03-19
2018
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2020
3
2018-09-14
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2018-09-14