| 137 | 4 | 42 |
| 下载次数 | 被引频次 | 阅读次数 |
为快速鉴定腮腺炎病毒核酸检测中由阳性对照引起的交叉污染问题,本研究建立了一种新型腮腺炎病毒核酸检测阳性对照。该阳性对照为合成的RNA转录产物,在使用相同的引物和反应条件下,该阳性对照在RT-PCR和巢式PCR试验中产生的PCR产物和正常腮腺炎病毒核酸阳性标本的PCR产物片段长度不同,通过比较PCR产物的大小可快速鉴定由阳性对照引起实验室交叉污染。这种新型腮腺炎病毒RNA阳性对照可广泛应用于腮腺炎病毒实验室诊断和基因定型中,对腮腺炎病毒核酸检测可进行良好的实验室质量控制。
Abstract：To rapidly identify the cross-contamination problems caused by the positive control in the process of mumps virus nucleic acid detection,a new mumps virus RNA positive control was developed in this study.Using the same primers and reaction conditions,the cross-contamination problems caused by the positive control could be readily identified by comparing the fragments lengths of the PCR products between the positive control and the samples.This new RNA positive control of mumps virus can be widely used in the diagnosis and genotyping of mumps virus as a better laboratory quality control.
[1]Jin L,Richards A,Brown D W.Development of a dualtarget-PCR for detection and characterization of measlesvirus in clinical specimens[J].Mol Cell Probes,1996,10(3):191-200.
[2]Jin L,Beard S,Brown D W.Genetic heterogeneity ofmumps virus in the United Kingdom:identification oftwo new genotypes[J].J Infect Dis,1999,180(3):829-833.
[3]Kubar A,Yapar M,Besirbellioglu B,et al.Rapid andquantitative detection of mumps virus RNA by one-stepreal-time RT-PCR[J].Diagn Microbiol Infect Dis,2004,49(2):83-88.
[4]Uchida K,Shinohara M,Shimada S,et al.Rapid andsensitive detection of mumps virus RNA directly fromclinical samples by real-time PCR[J].J Med Virol,2005,75(3):470-474.
[5]Jin L,Feng Y,Parry R,et al.Real-time PCR and itsapplication to mumps rapid diagnosis[J].J Med Virol,2007,79(11):1761-1767.
[6]Jin L,Brown D W,Litton P A,et al.Genetic diversityof mumps virus in oral fluid specimens:application tomumps epidemiological study[J].J Infect Dis,2004,189(6):1001-1008.
[7]Jin L,Vyse A,Brown D W.The role of RT-PCR assayof oral fluid for diagnosis and surveillance of measles,mumps and rubella[J].Bull World Health Organ,2002,80(1):76-77.
[8]Cohen B J,Jin L,Brown D W,et al.Infection withwild-type mumps virus in army recruits temporally asso-ciated with MMR vaccine[J].Epidemiol Infect,1999,123(2):251-255.
[9]Wehner H,Morris R,Logan M,et al.A secondaryschool outbreak of mumps following the childhood im-munization programme in England and Wales[J].Epide-miol Infect,2000,124(1):131-136.
[10]Cui A,Myers R,Xu W,et al.Analysis of the geneticvariability of the mumps SH gene in viruses circulatingin the UK between 1996and 2005[J].Infect GenetEvol,2009,9(1):71-80.
基本信息:
DOI：10.13242/j.cnki.bingduxuebao.002443
中图分类号:R440
引用信息:
[1]崔爱利,金俐,许文波.构建一种新型腮腺炎病毒核酸检测阳性对照品[J].病毒学报,2013,29(05):544-547.DOI:10.13242/j.cnki.bingduxuebao.002443.
基金信息:
国家科技重大专项课题(课题编号:2012ZX10004201-003和2013ZX10004202); 国家自然科学基金(课题编号:81102170)
2013-09-16
2013-09-16
2013-09-16