| 208 | 14 | 62 |
| 下载次数 | 被引频次 | 阅读次数 |
本研究通过对新疆地区不明原因发热人群血清Tahyna病毒抗体进行检测,以了解Tahyna病毒在当地的感染状况及分布特征。通过间接免疫荧光方法对新疆维吾尔自治区南部喀什地区、北部伊犁地区742例不明原因发热患者急性期血清标本进行Tahyna病毒抗体检测,并对IgM抗体阳性标本平行进行Tahyna病毒、Snowshoe hare病毒和Inkoo病毒三种抗原性相似的布尼亚病毒的蚀斑减少中和试验。研究结果显示新疆南部喀什地区采集不明原因发热患者急性期血清中,IgM抗体阳性率5.3%,IgG抗体阳性率18.3%;新疆北部伊犁地区采集的急性期患者血清标本中并未发现TAHV IgM抗体阳性;蚀斑减少中和试验结果显示,TAHV IgM抗体阳性患者血清中Tahyna病毒中和抗体滴度较其它两种布尼亚病毒中和抗体滴度升高明显。本研究证实新疆南部地区不明原因发热人群存在Tahyna病毒的急性感染和既往感染,为相关疾病的进一步监测提供基础。
Abstract：To investigate the infection status and the spatial distribution of Tahyna virus infection among unknown fever cases in Xinjiang,China.Sera samples of unknown fever cases from Kashi in southern Xinjiang and Yili in northern Xinjiang were tested against Tahyna virus by IFA.Partial positive cases were tested against Tahyna virus/Snowshoe hare virus/Inkoo virus parrelled.Finally,742 sera samples of unknown fever cases were collected from Kashi,Southern Xinjiang in 2007-2008,the positive rate of IgM antibody against Tahyna virus was 5.3%,the positive rate of IgG antibody against Tahyna virus was 18.3%.222 sera samples of unknown fever cases were collected from Yili,Northern Xinjiang in 2008,no positive case of IgM antibody against Tahyna was found.10 cases showed antibody neutralization against Tahyna virus by plaque reduction neutralization test.Our results demonstrate that there is current infection and past infection of Tahyna virus among Southern Xinjiang residents.
[1]Lu Z,Lu X J,Fu S H,et al.Tahyna virus and humaninfection,China[J].Emerg Infect Dis,2009,15:306-309.
[2]Li W J,Wang J L,Li M H,et al.Mosquitoes and mos-quito-borne arboviruses in the Qinghai-Tibet Plateau--fo-cused on the Qinghai area,China[J].Am J Trop MedHyg,2010,82:705-711.
[3]Ting S H,See E,Tan H C,et al.Development of asimplified assay for the detection of neutralizing antibod-ies to Japanese encephalitis virus[J].J Virol Methods,2001,93:43-47.
[4]JuricováZ,Bárdos V,Medek M,et al.Hemagglutina-tion-inhibition antibodies against the Tahyna virus in pa-tients in South Moravia[J].Cesk Epidemiol MikrobiolImunol,1983,32:349-354.
[5]Vanlandingham D L,Davis B S,Lvov D K,et al.Mo-lecular characterization of California serogroup viruses i-solated in Russia[J].Am J Trop Med Hyg,2002,67:306-309.
[6]Bardos V,Danielova V.The Tahyna virus-a virus isola-ted from mosquitoes in Czechoslovakia[J].J Hyg Epide-miol Microbiol Immunol,1959,3:264-276.
[7]Zdenek Hubálek.Mosquito-borne viruses in Europe[J].Parasitol Res,2008,103(S1):29-43.
[8]Gratz N.Vector-and rodent-borne diseases in Europeand North America:distribution,public health burdenand control[M].New York:Cambridge UniversityPress;2006.
[9]Acha P N,Szyfres B.Zoonoses and communicable dis-eases common to man and animals,3rd ed.Volume II:Chlamydioses,rickettsioses,and viruses[M].Washing-ton:Pan American Health Organization;2003.
[10]Gu H X,Artsob H.The possible presence of Tahyna(Bunyaviridae,California serogroup)virus in the Peo-ple's Republic of China[J].Trans R Soc Trop MedHyg,1987,81:693.
[11]Virus Taxonomy,Classification and Nomenclature ofViruses,8th ICTV Report of the International Com-mittee on Taxonomy of Viruses[C].Fauquet C M,Mayo M A,Maniloff,J,et al.2005,Elsevier/Aca-demic Press,1259.
基本信息:
DOI：10.13242/j.cnki.bingduxuebao.002137
中图分类号:R181.3
引用信息:
[1]吕志,付士红,王凤田,等.中国新疆维吾尔自治区不明原因发热人群中Tahyna病毒感染状况调查[J].病毒学报,2011,27(01):71-74.DOI:10.13242/j.cnki.bingduxuebao.002137.
基金信息:
科技部重大传染病专项(编号:2008ZX10004-001); 传染病国家重点实验室基金(编号:2008SKLID105); 中美新发和再发传染病合作项目(编号:U19-GH000004); 中国疾病预防控制中心青年科研基金(编号:2010A105)
2011-01-15
2011-01-15