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乙型肝炎是严重威胁人类健康的疾病之一。分析乙型肝炎病毒(Hepatitis B virus,HBV)的进化过程,可以获得HBV序列间的亲疏进化关系,进而为乙型肝炎的预测、治疗等方面研究提供依据。为了研究乙肝病毒样本的基因亚型,本文使用序列分析方法对采集到的临床HBV序列和公共核酸数据库的HBV数据集进行系统进化树构建和序列结构分析。结果显示,样本中有一条克隆序列的C基因区域是一种新的HBV B/C混合亚型。并且,实验结果还验证了云南省西双版纳州HBV新亚型HBV/B6的存在。本文的实验结论对云南省少数民族聚居地区乙型肝炎病毒的系统进化研究具有一定的价值。
Abstract：Hepatitis B is one of the most serious global threats to human health.Phylogenetic analysis of hepatitis B virus(HBV)can reveal the evolutionary relationship between HBV sequences and thus provide a basis for the prediction and treatment of hepatitis B and other aspects.In this study,we performed sequence analyses on the HBV sequences of five clinical HBV samples and the HBV sequences retrieved from the GenBank,EMBL,and DDBJ to construct a phylogenetic tree and analyze sequence structures.The experimental results revealed that the C gene of one cloned sequence had a recombinant structure of HBV B/ C subtype.Moreover,the phylogenetic results proved the existence of a newly found subtype HBV/B6in Xishuangbanna of Yunnan Province,China.The experimental conclusion represents certain value for phylogenetic studies of HBV in Yunnan ethnic minority groups.
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基本信息:
DOI：10.13242/j.cnki.bingduxuebao.000001
中图分类号:R373
引用信息:
[1]易青青,马磊,贾奇男,等.HBV新亚型中C基因片段的B/C型结构分析[J].病毒学报,2014,30(01):19-24.DOI:10.13242/j.cnki.bingduxuebao.000001.
基金信息:
国家自然科学基金资助项目(No:11265007); 教育部留学回国人员科研启动基金资助项目(No:2010-1561)
2014-01-24
2014-01-24
2014-01-24