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A full-length genome of a Chinese porcine reproductive and respiratory syndrome virus (PRRSV) isolate and designated as HB-1(sh)/2002,was sequenced and analyzed. The size of complete genome for the isolate is 15,411 nucleotides obtained from a set of overlapping cDNA clones excluding poly A tail,and is comprised of 9 ORFs,two of which encode nonstructural proteins and the others encode the structural proteins. The full-length sequence of HB-1(sh)/2002 was compared with that of two Chinese isolates,BJ-4 and CH-1a.Comparative analysis presented that the complete nucleotide sequence of HB-1(sh)/2002 shared 89.8% and 96.0% identity with BJ-4 and CH-1a,respectively.Compared with the other isolates,it seemed to be more extensive in Nsp2 of nonstructural regions and ORF5 of structural coding regions,respectively.Especially,Nsp2 only shared 77.3% and 89.1% identity with BJ-4 and CH-1a.The ORF5 shared 87.0% and 93.5% identity with BJ-4 and CH-1a.Phylogenetic analysis based on Nsp2 deduced amino acid sequence suggested that HB-1(sh)/2002 belongs to the same subgroup as CH-1a, and Nsp2 can be used as basis for monitoring the genetic variation and typing the PRRSV isolates further.
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基本信息:
DOI：10.13242/j.cnki.bingduxuebao.001642
中图分类号:S852.65
引用信息:
[1]高志强,郭鑫,陈艳红,查振林,杨汉春.猪繁殖与呼吸综合征病毒HB-1(sh)/2002株全基因组分子特征分析[J].病毒学报,2004(03):270-274.DOI:10.13242/j.cnki.bingduxuebao.001642 .
基金信息:
国家重点基础研究发展规划项目(G1999011901)
2004-09-30
2004-09-30