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G8基因型轮状病毒已在我国出现并迅速增加。本研究拟对采集于中国中南部5岁以下急性胃肠炎患儿的7例G8P[8]型RVA毒株进行全基因组分子特征分析。对轮状病毒特异性扩增结合高通量测序方法对中国中南部14株G8P[8]型RVA毒株进行全基因组测序。利用MEGA11.0、Geneious Prime、Geneious9.0.2和DNASTAR软件对RVA毒株进行全序列及遗传变异和进化分析。7株G8P[8]型RVA的基因分型为G8-P[8]-I2-R2-C2-M2-A2-N2-T2-E1-H2，显示为DS-1样基因型。系统进化树结果显示，本研究中国中南部7株G8P[8]分为两大谱系G8-I(13株)和谱系G8-Ⅱ（1株），VP7的最大分支可信度（MCC）树表明，G8的平均进化率估计为1.4×10-3替换/位点/年。G8P[8]基因型RVA在我国包括两个谱系，从该病毒在中国的出现到该病毒在中国的流行传播将经过1～2年，持续的全国RVA监测至关重要。
Abstract：Rotaviruses of the G8 genotype have emerged and increased rapidly in China. We undertook genomewide molecular characterization of seven G8P[8]-type RVA strains collected from children under 5 years of age with acute gastroenteritis in south-central China. Rotavirus-specific amplification combined with high-throughput sequencing was used to sequence the whole genome of 14 G8P[8]-type RVA strains from south-central China.Whole-sequence and genetic variation and evolutionary analyses of the RVA strains were carried out using MEGA11.0, Geneious Prime, Geneious9.0.2 and DNASTAR. Seven G8P[8]-type RVA strains were genotyped as G8-P[8]-I2-R2-C2-M2-A2-N2-T2-E1-H2, which was shown to be a DS-1-like genotype. The phylogenetic tree showed that the seven strains of G8P[8] in south-central China were divided into two major lineages: G8-I(13 strains) and lineage G8-II(1 strain). The maximum branching confidence(MCC) tree of VP7 indicated that the average evolutionary rate of G8 was estimated to be 1.4 × 10-3 substitutions/locus/year.In summary, the genotype of G8P[8] and RVA comprised two lineages in China. The average evolutionary rate was estimated to be 1.4 × 10-3 substitutions/location/year. The time of emergence to the epidemic spread of the virus in China will take 1–2 years, and continuous national surveillance of RVA is essential.
[1]Troeger C,Khalil I A,Rao P C,et al.Rotavirus Vaccination and the Global Burden of Rotavirus Diarrhea Among Children Younger Than 5 Years[J].JAMAPediatr,2018,172(10):958‐965.DOI:10.1001/jamapediatrics.2018.1960.
[2]Bányai K,LászlóB,Duque J,et al.Systematic review of regional and temporal trends in global rotavirus strain diversity in the pre rotavirus vaccine era:insights for understanding the impact of rotavirus vaccination programs[J].Vaccine,2012,30 Suppl 1:A122‐130.DOI:10.1016/j.vaccine.2011.09.111.
[3]DóróR,LászlóB,Martella V,et al.Review of global rotavirus strain prevalence data from six years post vaccine licensure surveillance:Is there evidence of strain selection from vaccine pressure?[J].Infect Genet Evol,2014,28:446‐461.DOI:10.1016/j.meegid.2014.08.017.
[4]Kondo K,Tsugawa T,Ono M,et al.Clinical and molecular characteristics of Human Rotavirus G8P[8]outbreak strain,Japan,2014[J].Emerg Infect Dis.2017,23(6):968‐972.DOI:10.3201/eid2306.160038.
[5]Matthijnssens J,Ciarlet M,Rahman M.Recommendations for the classification of group Arotaviruses using all 11 genomic RNA segments[J].Arch Virol,2008,153(8):1621‐1629.DOI:10.1007/s00705‐008‐0155‐1.
[6]Matthijnssens J,Ciarlet M,Mcdonald S M,et al.Uniformity of rotavirus strain nomenclature proposed by the Rotavirus Classification Working Group (RCWG)[J].Arch Virol,2011,156(8):1397‐1413.
[7]中华预防医学会.儿童轮状病毒胃肠炎免疫预防专家共识（2020版）[J].中国疫苗和免疫，2021,27(01):48‐61.DOI:10.19914/j.CJVI.2021002..
[8]Mijatovic‐Rustempasic S,Bányai K,Esona M D,et al.Genome sequence based molecular epidemiology of unusual US Rotavirus A G9 strains isolated from Omaha,USA between 1997 and 2000[J].Infect Genet Evol,2011,11 (2):522‐527.DOI:10.1016/j.meegid.2010.11.012.
[9]Drummond A J,Suchard M A,Xie D,et al.Bayesian phylogenetics with BEAUti and the BEAST 1.7[J].Mol Biol Evol,2012,29(8):1969‐1973.DOI:10.1093/molbev/mss075.
[10]McDonald,Sarah M,Nelson,et al.Reassortment in segmented RNA viruses:mechanisms and outcomes[J].Nat Rev Microbiol,2016,14(7):448‐460.DOI:10.1038/nrmicro.2016.46
[11]Taniguchi K,Urasawa T,Urasawa S.Independent segregation of the VP4 and the VP7 genes in bovine rotaviruses as confirmed by VP4 sequence analysis of G8and G10 bovine rotavirus strains[J].J Gen Virol,1993,74 (Pt 6):1215‐1221.DOI:10.1099/0022‐1317‐74‐6‐1215.
[12]Hasegawa A,Inouye S,Matsuno S,et al.Isolation of Human Rotaviruses with a Distinct RNA Electrophoretic Pattern from Indonesia[J].Microbiol Immunol,1984,28(6):719‐722.DOI:10.1111/j.1348‐0421.1984.tb00726.x.
[13]Jagannath M R,Vethanayagam R R,Reddy B S Y,et al.Characterization of human symptomatic rotavirus isolates MP409 and MP480 having'long'RNAelectropherotype and subgroup I specificity,highly related to the P6[1],G8 type bovine rotavirus A5,from Mysore,India[J].Arch Virol,2000,145(7):1339‐1357.DOI:10.1007/s007050070094.
[14]Modaress S,Rahbarimanesh A A,Edalat R,et al.Human rotavirus genotypes detection among hospitalized children,a study in Tehran,Iran[J].Arch Iran Med,2011,14(1):39‐45.DOI:011141/AIM.009.
[15]Hoa‐Tran TN,Nakagomi T,Vu HM,et al.Abrupt emergence and predominance in Vietnam of rotavirus Astrains possessing a bovine‐like G8 on a DS‐1‐like background[J].Arch Virol,2016,161(2):479‐482.DOI:10.1007/s00705‐015‐2682‐x.
[16]Ratana T,Satoshi K,Ratigorn G,et al.Full Genome Characterization of Novel DS‐1‐Like G8P[8]Rotavirus Strains that Have Emerged in Thailand:Reassortment of Bovine and Human Rotavirus Gene Segments in Emerging DS‐1‐Like Intergenogroup Reassortant Strains[J].PLoS One.2016,11 (11):e0165826.DOI:10.1371/journal.pone.0165826.
[17]Gabriel C,Ho H J,Ching‐Ging N,et al.An unusual outbreak of rotavirus G8P[8]gastroenteritis in adults in an urban community,Singapore,2016[J].J Clin Virol,2018,105:57‐63.DOI:10.1016/j.jcv.2018.06.004.
[18]Phan T,Kobayashi M,Nagasawa K.Whole genome sequencing and evolutionary analysis of G8P[8]rotaviruses emerging in Japan[J].Virusdisease,2022,33(2):215‐218.DOI:10.1007/s13337‐022‐00765‐z.
[19]Wang SJ,Chen LN,Wang SM,et al..Genetic characterization of two G8P[8]rotavirus strains isolated in Guangzhou,China,in 2020/21:evidence of genome reassortment[J].BMC Infect Dis,2022,22(1):579.DOI:10.1186/s12879‐022‐07542‐9.
[20]Degiuseppe J I,Stupka J A,Network A R S.Emergence of unusual rotavirus G9P[4]and G8P[8]strains during post vaccination surveillance in Argentina,2017-2018[J].Infect Genet Evol,2021,93:104940.DOI:10.1016/j.meegid.2021.104940.
[21]Hoque S A,Kobayashi M,Takanashi S,et al.Role of rotavirus vaccination on an emerging G8P[8]rotavirus strain causing an outbreak in central Japan[J].Vaccine,2018,36 (1):43‐49.DOI:10.1016/j.vaccine.2017.11.056.
基本信息:
DOI：10.13242/j.cnki.bingduxuebao.004452
中图分类号:R373.2
引用信息:
[1]熊光萍,田毅,范佳欣,等.中国中南部G8P[8]型A组轮状病毒全基因组遗传特征研究[J].病毒学报,2024,40(01):27-37.DOI:10.13242/j.cnki.bingduxuebao.004452.
基金信息:
国家重点研发计划资助（项目号：2022YFC2304302）,题目：呼吸道疾病非注射剂型疫苗研发及靶向递送技术体系研究; 国家自然科学基金（项目号：21934005）,题目：中国母乳糖组高效分离分析及轮状病毒蛋白相互作用研究~~
2024-01-19
2024-01-19
2024-01-19