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本实验室自2011年开始在鄱阳湖周边地区开展家禽养殖户和活禽市场环境中的禽流感病毒监测,在2011~2016年的监测过程中,通过病毒分离和深度测序,发现了一株H12N2亚型禽流感病毒,这是在中国首次检出该亚型病毒。该标本来自一份鸭粪便标本,采集自江西省余干县的一个家禽养殖场。病毒的8个片段分别与来自鸭或者野禽中的病毒最为接近。对病毒的全基因组系统进化分析表明,该病毒属于欧亚谱系。HA蛋白裂解位点序列为PQAQDR/GLF,属于低致病性禽流感病毒,受体结合位点分析表明病毒对α-2,3唾液酸受体亲和力较好。病毒NA的茎部没有氨基酸的缺失,但是PB1蛋白的L13P和L473V可以增强病毒聚合酶在哺乳动物中复制的能力和病毒对于哺乳动物的毒性。M1蛋白N30D突变和T215A突变提示会增加流感病毒对于哺乳动物的致病性。本次从家禽养殖场环境中检出野鸟相关的禽流感病毒进一步说明了在该地区进行禽流感病毒监测的重要性,以及在家禽-野鸟界面加强生物安全措施的必要性,需要持续进行监测,为禽流感病毒的暴发提供预警,为人感染禽流感病毒提供风险评估的依据。
Abstract：The Chinese National Influenza Center has conducted the surveillance of avian influenza in the Poyang Lake Region since 2011,using environmental samples from poultry farms and live poultry markets.During surveillance from 2011 to 2016,we identifiedone H12N2 influenza virus using virus isolation and deep sequencing methods.This is the first report of detection of an influenza H12N2 subtype virus in China.The specimen was a feces sample from a duck farm located in Yugan County in Jiangxi Province.Phylogenetic analyses showed that all eight segments of the H12N2 virus belonged to a Eurasian lineage,similar to avian influenza viruses originating from ducks or wild birds.The sequence at the HA cleavage site was PQAQDR/GLF,which suggested that this was a low-pathogenic avian influenza virus.Analyses of receptor binding sites suggested that this virus could binding strongly toα-2,3-linked sialic acid receptor.There was no amino-acid deletion in the stalk of the NA protein of this virus,whereasL13 Pand L473Vin the PB1 protein could increase the polymerase activity,replication efficiency,and virulence in mammals.N30 Dand T215Asubstitutions in the M1 protein suggestedincreased virulence in mammals.Such detection of a wild bird-related influenza virus in environmental samples frompoultry farms further emphasizes the importance of surveillance of avian influenza viruses in this region and the necessity to enhance biosafety measures at the poultry-wild bird interface.Conduct continuous surveillance is needed to provide early warnings for influenza outbreaks in poultry,and evidence for the risk assessment of human infection with avian influenza viruses.
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基本信息:
DOI：10.13242/j.cnki.bingduxuebao.003111
中图分类号:S852.65
引用信息:
[1]张恒,袁辉,陈涛,等.中国首次检出的H12N2亚型禽流感病毒的基因组特征和系统进化分析[J].病毒学报,2017,33(02):129-135.DOI:10.13242/j.cnki.bingduxuebao.003111.
基金信息:
国家重点研发计划(项目号:2016YFD0500208),题目:人感染动物流感病毒(项目号:2016YFC1200200),题目:重要新发突发病原体发生与播散机制研究~~
2017-03-29
2017-03-29
2017-03-29