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分析埃可病毒11型(Echovirus 11,ECHO 11)福建龙岩分离株的分子生物学特征。收集龙岩市第一医院2011年1~12月临床诊断为病毒性脑炎或中枢神经系统感染的住院病例脑脊液标本进行病毒分离鉴定,从7株经血清中和鉴定的ECHO 11分离株中,选取4株测定VP1完整编码区序列,与GenBank上已发表的ECHO 11型病毒VP1区进行同源性比较及遗传进化分析。4株ECHO 11分离株VPl区序列长度为600个核苷酸,编码200个氨基酸;4株之间的核苷酸同源性为100%,氨基酸同源性为99%~100%;与1953年Gregory原型株之间的核苷酸同源性为75%~76%,氨基酸同源性为90%;与2007年荷兰株(GU393773)之间核苷酸的同源性为94%~95%,氨基酸同源性为98%~99%,同源性最高;与国内2010年山东株之间核苷酸的同源性为74%,氨基酸同源性为88%~89%。系统进化树分析显示,4株龙岩分离株同属D5型,其与D5型病毒株(GU393713)之间核苷酸的同源性为93%,氨基酸同源性为99%。国内分离株之间相对较低的相似性提示国内ECHO 11病毒可能存在不同的传播链。
Abstract：This study aimed to analyse the genetically characterize isolates of Echovirus 11 from Longyan City,Fujian Province,and to reveal their genetic relationships with other isolates from China and abroad.Cerebrospinal fluid specimens from patients diagnosed with viral encephalitis or central nervous system(CNS)infections were collected from Longyan First Hospital between January and December 2011.Seven Echo11 strains were isolated and identified using the RIMV serum panel.The entire VP1 coding regions of four strains were sequenced and typed as Echo11 by an online blast program and,subsequently,phylogenetic analyses of the VP1 sequences of these stains and others published on GenBank were conducted.There were 600nucleotides(nt)in each complete VP1 coding region that encoded 200 amino acids(aa).Among those four Echo11 strains,the sequence identities of nt and aa were 100% and 99%-100%,respectively.And phylogenetic analyses indicate belong to subtype DS,the homology compared with DS strain(GU393713)were 93%(nt)and 99%(aa).The sequence identities for the nt and aa were 75%-76%and90%,respectively,between the current isolates from Longyan and the Gregory prototype strain found in1953.The sequence identity of nt and aa between the Longyan virus strains and the domestic Shandong strains isolated in 2010 were lower,at 74% and 88%-89%,respectively.However,the highest level of homology was found when the Longyan strains were compared with the Netherlands strain(GU393773)found in 2007(nt and aa identity:94%-95% and 98%-99%,respectively).The relatively low levels of similarity between domestic isolates suggest that different transmission routes exist for Echo11 in mainland China.
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基本信息:
DOI：10.13242/j.cnki.bingduxuebao.002623
中图分类号:R512.3;R373
引用信息:
[1]陈前进,曹春远,张彦锋,等.病毒性脑炎病例中埃可病毒11型病毒的基因特征分析[J].病毒学报,2015,31(01):36-41.DOI:10.13242/j.cnki.bingduxuebao.002623.
基金信息:
福建省卫生厅青年科研课题项目(2012-2-126)
2015-01-30
2015-01-30
2015-01-30