| 624 | 1 | 79 |
| 下载次数 | 被引频次 | 阅读次数 |
免疫PCR(Immuno-PCR,IPCR)技术是一种结合了酶联免疫吸附测定（Enzyme linked immunosorbent assay,ELISA）的特异性和聚合酶链式反应（Polymerase chain reaction,PCR）的高灵敏度的检测方法。与ELISA相比，IPCR由于PCR技术指数扩增的能力，检测灵敏度可以达到fg/mL。由于具有高灵敏度，IPCR在微量非核酸分子检测方面有着广泛的应用前景。本文主要介绍了传统IPCR和改良IPCR的原理和优缺点，概述了IPCR技术在病毒、细菌、寄生虫、肿瘤标志物、真菌、食品等方面的应用进展，总结了影响IPCR技术检测的因素并展望了IPCR技术的发展趋势。
Abstract：Immuno-polymerase chain reaction(IPCR) is a method that combines the specificity of enzyme-linked immunosorbent assay(ELISA) and the high sensitivity of polymerase chain reaction(PCR). Compared with ELISA, the sensitivity of IPCR can reach fg/mL due to the exponential amplification ability of PCR technology. Due to its high sensitivity, IPCR has a wide range of applications in detection of trace biomolecules.This review introduces the development process of IPCR, various forms of IPCR, and application of IPCR in virus, bacteria, parasites, tumor markers, fungi, environmental detection, etc., all of these prove that IPCR technology has high application value in the detection of trace biomolecules.
[1] Assumpcao AL, da Silva RC. Immuno-PCR in cancer and non-cancer related diseases:a review[J]. Vet Q,2016, 36(2):63-70. DOI:10.1080/01652176.2016.1164912.
[2] Spengler M, Adler M, Niemeyer CM. Highly sensitive ligand-binding assays in pre-clinical and clinical applications:immuno-PCR and other emerging techniques[J]. Analyst, 2015, 140(18):6175-6194.DOI:10.1039/c5an00822k.
[3] Malou N, Raoult D. Immuno-PCR:a promising ultrasensitive diagnostic method to detect antigens and antibodies[J]. Trends Microbiol, 2011, 19(6):295-302. DOI:10.1016/j.tim.2011.03.004
[4] Tabatabaei MS, Islam R, Ahmed M. Applications of gold nanoparticles in ELISA, PCR, and immuno-PCR assays:A review[J]. Anal Chim Acta, 2021, 1143:250-266. DOI:10.1016/j.aca.2020.08.030.
[5] Mehta PK, Raj A, Singh NP, et al. Detection of potential microbial antigens by immuno-PCR(PCRamplified immunoassay)[J]. J Med Microbiol,2014,63(Pt5):627-641. DOI:10.1099/jmm.0.070318-0.
[6] Sano T, Smith CL, Cantor CR. Immuno-PCR:very sensitive antigen detection by means of specific antibodyDNA conjugates[J]. Science, 1992, 258(5079):120-122. DOI:10.1126/science.1439758.
[7]黄杰，李金明.免疫PCR技术及其应用研究进展[J].国际检验医学杂志，2006,(08):709-712. DOI:10.3969/j.issn.1673-4130.2006.08.015.
[8] Ryazantsev DY, Voronina DV, Zavriev SK. ImmunoPCR:Achievements and Perspectives[J]. Biochemistry(Mosc), 2016, 81(13):1754-1770. DOI:10.1134/S0006297916130113.
[9] Zhou H, Fisher RJ, Papas TS. Universal immuno-PCR for ultra-sensitive target protein detection[J]. Nucleic Acids Res, 1993, 21(25):6038-6039. DOI:10.1093/nar/21.25.6038.
[10]Guo YC, Zhou YF, Zhang XE, et al. Phage display mediated immuno-PCR[J]. Nucleic Acids Res, 2006,34(8):e62. DOI:10.1093/nar/gkl260.
[11]He J, Evers DL, O'Leary TJ, et al. ImmunoliposomePCR:a generic ultrasensitive quantitative antigen detection system[J]. J Nanobiotechnology, 2012, 10(1):26. DOI:10.1186/1477-3155-10-26.
[12]Jayathilake C, Terai T, Nemoto N. cDNA Display Mediated Immuno-PCR(cD-IPCR):A Novel PCRbased Antigen Detection Method[J]. Bio Protoc,2019, 9(24):e3457. DOI:10.21769/BioProtoc.3457.
[13]Jayathilake C, Nemoto N. cDNA Display-Mediated Immuno-PCR(cD-IPCR):An Ultrasensitive Immunoassay for Biomolecular Detection[J]. Methods Mol Biol, 2021, 2261:307-321. DOI:10.1007/978-1-0716-1186-9_19.
[14]Anzai H, Terai T, Jayathilake C, et al. A novel immuno-PCR method using cDNA display[J]. Analytical Biochemistry, 2019, 578:1-6. DOI:10.1016/j.ab.2019.04.017.
[15]Service RF. Materials science. Tiny particles flag scarce proteins[J]. Science, 2003, 301(5641):1827. DOI:10.1126/science.301.5641.1827a.
[16]刘培，尹会敏，穆文静，等.生物条形码检测技术及其研究进展[J].生物安全学报，2021, 30(03):172-177.DOI:10.3969/j.issn.2095-1787.2021.03.003.
[17]贺明星，李凯，周宇荀，等.纳米生物条形码技术在分子诊断中的应用[J].生命的化学，2008,(01):86-88.DOI:10.3969/j.issn.1000-1336.2008.01.027.
[18]Zhang P, Chen L, Hu J, et al. Magnetofluidic immunoPCR for point-of-care COVID-19 serological testing[J].Biosens Bioelectron, 2022, 195:113656. DOI:10.1016/j.bios.2021.113656.
[19]Barletta J, Bartolome A, Constantine NT.Immunomagnetic quantitative immuno-PCR for detection of less than one HIV-1 virion[J]. J Virol Methods, 2009, 157(2):122-132. DOI:10.1016/j.jviromet.2008.12.013.
[20]Monjezi R, Tan SW, Tey BT, et al. Detection of hepatitis B virus core antigen by phage display mediated TaqMan real-time immuno-PCR[J]. J Virol Methods,2013, 187(1):121-126. DOI:10.1016/j.jviromet.2012.09.017.
[21]Adler M, Schulz S, Fischer R, et al. Detection of Rotavirus from stool samples using a standardized immuno-PCR("Imperacer")method with end-point and real-time detection[J]. Biochem Biophys Res Commun, 2005, 333(4):1289-1294. DOI:10.1016/j.bbrc.2005.06.027.
[22]Yin HQ, Ji CF, Yang XQ, et al. An improved gold nanoparticle probe-based assay for HCV core antigen ultrasensitive detection[J]. J Virol Methods, 2017,243:142-145. DOI:10.1016/j.jviromet.2017.02.007.
[23]Perez JW, Vargis EA, Russ PK, et al. Detection of respiratory syncytial virus using nanoparticle amplified immuno-polymerase chain reaction[J]. Anal Biochem,2011, 410(1):141-148. DOI:10.1016/j.ab.2010.11.033.
[24]Chen L, Wei H, Guo Y, et al. Gold nanoparticle enhanced immuno-PCR for ultrasensitive detection of Hantaan virus nucleocapsid protein[J]. J Immunol Methods, 2009, 346(1-2):64-70. DOI:10.1016/j.jim.2009.05.007.
[25]Pivovarov VD, Ryazantsev DY, Simonova MA, et al.Immuno-PCR Assay for Quantitation of Antibodies to Epstein-Barr Virus[J]. Mol Biol(Mosk), 2018, 52(4):727-734. DOI:10.1134/S0026898418040158.
[26]Deng MJ, Xiao XZ, Zhang YM, et al. A highly sensitive immuno-PCR assay for detection of H5N1avian influenza virus[J]. Molecular Biology Reports,2010, 38(3):1941-1948. DOI:10.1007/s11033-010-0315-8.
[27]Singh N, Dahiya B, Radhakrishnan VS, et al. Detection of Mycobacterium tuberculosis purified ESAT-6(Rv3875)by magnetic bead-coupled gold nanoparticlebased immuno-PCR assay[J]. Int J Nanomedicine,2018, 13:8523-8535. DOI:10.2147/IJN.S181052.
[28]Sharma S, Sheoran A, Gupta KB, et al. Quantitative detection of a cocktail of mycobacterial MPT64 and PstS1 in tuberculosis patients by real-time immuno-PCR[J]. Future Microbiol, 2019, 14:223-233. DOI:10.2217/fmb-2018-0284.
[29]Mehta PK, Sharma S, Mehta N, et al. Quantitative detection of mycobacterial mannophosphoinositides in tuberculosis patients by real-time immuno-PCR assay[J]. J Microbiol Methods, 2022, 201:106563. DOI:10.1016/j.mimet.2022.106563.
[30]Khan A, Singh R, Sharma S, et al. Diagnosis of osteoarticular tuberculosis by immuno-PCR assay based on mycobacterial antigen 85 complex detection[J]. Lett Appl Microbiol, 2022, 74(1):17-26. DOI:10.1111/lam.13567.
[31]Kamra E, Sharma S, Sheoran A, et al. Identification of mycobacterial MPT-64 and ESAT-6 proteins in urogenital tuberculosis patients by real-time immunoPCR[J]. Future Microbiol, 2022, 17:829-842. DOI:10.2217/fmb-2022-0037.
[32]Subbaram K, Gatasheh MK, Al Azzam KM, et al.Molecular identification of diarrheal Aeromonas using immuno magnetic polymerase chain reaction(IM-PCR)technique:a comparative study with conventional culture method[J]. Afr Health Sci, 2019, 19(2):2036-2042.DOI:10.4314/ahs.v19i2.27.
[33]Zhang W, Bielaszewska M, Pulz M, et al. New immuno-PCR assay for detection of low concentrations of shiga toxin 2 and its variants[J]. J Clin Microbiol, 2008, 46(4):1292-1297. DOI:10.1128/JCM.02271-07.
[34]He X, Qi W, Qui?ones B, et al. Sensitive detection of Shiga Toxin 2 and some of its variants in environmental samples by a novel immuno-PCR assay[J]. Appl Environ Microbiol, 2011, 77(11):3558-3564. DOI:10.1128/AEM.02205-10.
[35]Malou N, Tran TN, Nappez C, et al. Immuno-PCR--a new tool for paleomicrobiology:the plague paradigm.[J]. PLoS ONE, 2012, 7(2). DOI:10.1371/journal.pone.0031744.
[36]Liang H, Cordova SE, Kieft TL, et al. A highly sensitive immuno-PCR assay for detecting Group A Streptococcus[J]. J Immunol Methods, 2003, 279(1-2):101-110. DOI:10.1016/s0022-1759(03)00247-3.
[37]Huang SH, Chang TC. Detection of Staphylococcus aureus by a sensitive immuno-PCR assay[J]. Clin Chem, 2004, 50(9):1673-1674. DOI:10.1373/clinchem.2004.033548.
[38]Panneerseelan L, Muriana PM. An immunomagnetic PCR signal amplification assay for sensitive detection of Staphylococcus aureus enterotoxins in foods[J]. J Food Prot, 2009, 72(12):2538-2546. DOI:10.4315/0362-028x-72.12.2538.
[39]Das S, Majumder S, Nag M, et al. A sandwich duplex immuno PCR for rapid and sensitive identification of Clostridium perfringens alpha and enterotoxin[J].Anaerobe, 2019, 57:63-74. DOI:10.1016/j.anaerobe.2019.03.015.
[40]Deng MJ, Ji XC, Xiao XZ, et al, Zheng XL, Wang Q,Zhu LH. Immuno-PCR for detection of Giardia lamblia cysts in water[J]. J AOAC Int, 2014, 97(2):561-566.DOI:10.5740/jaoacint.13-069.
[41]Chye SM, Lin SR, Chen YL, et al. Immuno-PCR for detection of antigen to Angiostrongylus cantonensis circulating fifth-stage worms[J]. Clin Chem, 2004, 50(1):51-57. DOI:10.1373/clinchem.2003.020867.
[42]Mardani-Kataki M, Beiromvand M, Teimoori A, et al.Is Immuno-PCR Better than ELISA Test for Detection of Toxoplasma gondii IgG Antibody?[J]. Acta Parasitol, 2022, 67(2):904-911. DOI:10.1007/s11686-022-00537-1.
[43]Sadhasivam S, Chen JC, Savitha S, et al. Application of carbon nanotubes layered on silicon wafer for the detection of breast cancer marker carbohydrate antigen 15-3 by immuno-polymerase chain reaction[J]. J Mater Sci Mater Med, 2014, 25(1):101-111. DOI:10.1007/s10856-013-5060-9.
[44]Li ZG, Wang XY, Chang JL, et al. The establishment of supramolecular immunobead real-time PCR and the identification of Cox-2 as a metastasis-related marker in colorectal carcinoma[J]. Oncol Rep, 2012, 28(3):977-984. DOI:10.3892/or.2012.1867.
[45]Wang TW, Lu HY, Lou PJ, et al. Application of highly sensitive, modified glass substrate-based immunoPCR on the early detection of nasopharyngeal carcinoma[J]. Biomaterials, 2008, 29(33):4447-4454. DOI:10.1016/j.biomaterials.2008.07.015.
[46]Ren X, Zhang Q, Wu W, et al. Anti-idiotypic nanobody-phage display-mediated real-time immuno-PCR for sensitive, simultaneous and quantitative detection of total aflatoxins and zearalenone in grains[J]. Food Chem, 2019, 297:124912. DOI:10.1016/j.foodchem.2019.05.186.
[47]Attallah C, Rodríguez MC, Lozano V, Etcheverrigaray M, Oggero M. Design and validation of an immunoPCR assay for IFN-alpha2b quantification in human plasma[J]. Bioanalysis, 2019, 11(23):2175-2188.DOI:10.4155/bio-2019-0225.
[48]Huang W, Tu Z, Ning Z, et al. Development of RealTime Immuno-PCR Based on Phage Displayed an AntiIdiotypic Nanobody for Quantitative Determination of Citrinin in Monascus[J]. Toxins(Basel), 2019, 11(10). DOI:10.3390/toxins11100572.
[49]Tao X, He Z, Cao X, et al. Development of a highly sensitive real-time immuno-PCR for the measurement of chloramphenicol in milk based on magnetic bead capturing[J]. Anal Methods, 2014, 6(23):9340-9347.DOI:10.1039/c4ay02158d.
[50]杨光昕.基于免疫PCR生物条形码技术检测多氯联苯的研究[D/OL]；上海交通大学，2016.
[51]Bu D, Zhuang H. A real-time immuno-PCR assay for the flame retardant tris(2, 3-dibromopropyl)isocyanurate using a probe DNA conjugated to gold nanoparticles[J]. Microchimica Acta, 2014, 182(9-10):1863-1868. DOI:10.1007/s00604-014-1416-1.
[52]Timms M, Ganio K, Forbes G, et al. An immuno polymerase chain reaction screen for the detection of CJC-1295 and other growth-hormone-releasing hormone analogs in equine plasma[J]. Drug Testing and Analysis, 2018, 11(6):804-812. DOI:10.1002/dta.2554.
基本信息:
DOI：10.13242/j.cnki.bingduxuebao.004417
中图分类号:R392
引用信息:
[1]赵萌,申辛欣,冯志山.免疫PCR技术在微量非核酸分子检测方面的应用进展[J].病毒学报,2024,40(01):197-205.DOI:10.13242/j.cnki.bingduxuebao.004417.
基金信息:
国家重点研发计划（项目号：2021YFC2301100）,题目：病原多场景实时检测关键技术平台的建立和应用; 国家自然科学基金（项目号：82202593）,题目：重组酶介导的核酸等温扩增（RAA）检测体系的优化及其在新型冠状病毒和流感病毒A/B型检测中的应用~~
2023-09-15
2023
2023-10-19
2023-12-28
2023
1
2023-11-22
2023-11-22
2023-11-22