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为了解长白县黑线姬鼠中汉坦病毒流行情况及病毒型别,采用巢式RT-PCR方法筛查鼠肺RNA,并对PCR阳性样本进行全S基因的扩增、克隆及测序;构建系统发生树并进行分子进化分析。结果显示:共捕获黑线姬鼠58只,共检测出4份阳性标本,阳性率6.90%。经过序列测定及进化分析显示黑线姬鼠所携带的病毒与汉滩病毒第6基因亚型标准株核苷酸的同源性为95.8%～96.3%,氨基酸同源性为98.6%～99.5%。同时发现,长白县黑线姬鼠携带的汉滩病毒的NP蛋白共有1个特异性氨基酸位点为S387。
Abstract：To gain more insights into epidemiologic characteristics and genotype of hantavirus in Apodemus agrarius in Changbai Area.Complete hantavirus S segment sequences were amplified by RT-PCR and sequenced.The phylogenetic trees were constructed for analysis of genetic characters of hantavirus.A total of 58 Apodemus agrarius were trapped in the epidemic areas,and complete hantavirus S segment sequences were obtained from 4 lung samples of these rodents(6.90%).Phylogenetic analysis of the four S segment sequences indicated that all viruses isolated from Apodemu sagrarius were closely related to genotype 6 of Hantaan virus(95.8%-96.3%,nucleotide identity;98.6%-99.5%,amino acid identity),all of them had a specific S387 different from other genotypes of Hantaan virus.
[1]Plyusnin A,Vapalahti O,Vaher A.Hantaviruses:ge-nomestructure,expression and evolution[J].J GenVirol,1996,77(11):2677-2687.
[2]Khaiboullina S F,Morzunov S P,St Jeor S C.Hanta-viruses:molecular biology,evolution and pathogenesis[J].Current Molecular Medicine,2005,5,773-790.
[3]Rodriguez L L,Owens J H,Peters C J,et al.Geneticreassortment among viruses causing hantavirus pulmo-nary syndrome[J].Virology.1998,242(1):99-106.
[4]Plyusnin A,Morzunov S P.Virus evolution and geneticdiversity of hantaviruses and their rodent hosts[J].Cur-rent topics in microbiology and immunology.2001,256:47-75.
[5]Carey D E,Reuben R,Panicker K N,et al.Thottapa-layam virus:apresumptive arbovirus isolated from ashrew in India.Indian J Med Res,1971,59(11):1758-1760.
[6]Bohlman M C,Morzunov S P,Meissner J,et al.Anal-ysis of hantavirus genetic diversity in Argentina:S seg-ment-derived phylogeny[J].J Virol,2002,76(8):3765-3773.
[7]吴燕平,黄飚,扈光伟,等.吉林省肾综合症出血热流行趋势分析[J].中国地方病防治杂志,2004,19:164-165.
[8]Zeier M,Handermann M,Bahr U,et al.New ecologi-cal aspects of hantavirus infection:a change of a para-digm and a challenge of prevention--a review[J].Virusgenes,2005,30(2):157-180.
[9]Zou Y,Hu J,Wang Z X,et al.Molecular diversity andphylogeny of Hantaan virus in Guizhou,China:evi-dence for Guizhou as a radiation center of the presentHantaan virus[J].J Gen Virol,2008,89(8):1987-1997.
[10]刘江秋,范永星,蔡增林,等.吉林省长白县野鼠携带肾综合征出血热抗原和抗体的调查[J].沈阳部队医药,2001,14:28-29.
[11]刘刚,李川,扈光伟,等.在中国发现普马拉病毒[J].中华实验和临床病毒学杂志,2001,17:55-57.
[12]Zhang Y Z,Zou Y,Yan Y Z,et al.Detection of phy-logenetically distinct Puumala-like viruses from red-grey vole Clethrionomys rufocanus in China[J].J MedVirol,2007,79(8):1208-1218.
[13]赵臣,赵阳,李艳,等.吉林地区HFRS病原体基因型分析[J].微生物学杂志,2011,31:101-104.
[14]沈博,吴东林,朴东风,等.吉林省珲春市3株肾综合征出血热汉城型病毒的分离与鉴定[J].中国卫生工程学,2008,8:20-23.
[15]丁铲.病毒的进化与传播[J].中国动物传染病学报,2010,8:71-75.
基本信息:
DOI：10.13242/j.cnki.bingduxuebao.002405
中图分类号:S852.65
引用信息:
[1]燕清丽,杨鹏飞,邵丽筠,等.长白县黑线姬鼠携带汉坦病毒的S基因特征研究[J].病毒学报,2013,29(04):382-385.DOI:10.13242/j.cnki.bingduxuebao.002405.
基金信息:
国家质检总局质检公益项目(NO.2011IK150); 公益性科研院所基础研究基金项目(2011JK008)
2013-06-20
2013-06-20
2013-06-20