| 501 | 4 | 51 |
| 下载次数 | 被引频次 | 阅读次数 |
犬细小病毒(Canine parvovirus,CPV)具有高度传染性,是幼犬最危险的消化道传染病病原之一。为了解宁夏地区CPV的流行现状及其VP2基因的遗传变异情况,本研究采集了14份经胶体金试纸条检测为CPV阳性的腹泻犬粪样,进行病毒分离和鉴定,并将分离株VP2基因全序列进行克隆和测序分析。结果显示,14份样品中成功分离到5株CPV;病毒TCID50在104.9/0.1mL～105.4/0.1mL之间;HA效价均达1∶512以上;序列分析显示,5株分离株中3株为new CPV-2a型,2株为new CPV-2b型;分离株与参考株的核苷酸和氨基酸相似性分别为98.2%～99.9%和96.9%～99.8%;聚类分析显示,分离株序列全部聚类在同一分支。本研究的结果为我国西北地区CPV分离株的分子流行病学和遗传多样性提供了依据,有助于该地区预防和控制CPV感染。
Abstract：The canine parvovirus(CPV)is highly infectious and is one of the most dangerous digestive tract infectious diseases in puppies. We wished to study the prevalence of the CPV and variation of the VP2 gene in Ningxia Hui Autonomous Region,China. Fourteen feces samples of diarrhea dogs with CPV positive tested by colloidal gold test strips were collected and virus isolation and identification experiments were conducted. Then we cloned and analyzed the whole gene sequence of VP2. Results showed that 5 strains of CPV were successfully isolated from 14 samples,the virus TCID50 was 104.9/0.1 mL to 105.4/0.1 mL,the HA titer were higher than 1∶512. Sequence analysis showed that among the 5 isolates,3 strains were new CPV-2 a and 2 strains were new CPV-2 b. The nucleotide and amino acid sequence similarity between isolates and reference strains were 98.2%～99.9% and 96.9%～99.8%,respectively. Cluster analysis showed that the isolates sequences were all clustered in the same cluster. These results provide a basis for understanding the molecular epidemiology and genetic diversity of CPV isolates in Northwest China. Our data could contribute to the prevention and control of CPV infection in this area.
[1] Zhang Q Y,Qiu Y,Pan Z H,Wang S C,Wang B,Wu W K,Yu J M,Yi Y,Sun F L,Wang K C.Genome sequence characterization of canine parvoviruses prevalent in the Sichuan Province of China[J].Transbound Emerg Dis,2019,66(2):897-907.
[2]曹雪峰，彭广能，黄祥明，王文博，田一男，徐晓阳，雷霜霜，杨颜铱，弓超，杨奎兴，袁博，任志华，钟志军.腹泻犬中细小病毒携带情况以及VP2基因进化分析[J].病毒学报，2016,32(6):733-739. DOI:10.13242/j.cnki.bingduxuebao.003070
[3] Zou P, Zeng W J, Zhang X, Li S J. The genetic evolution of canine parvovirus-A new perspective[J/OL]. PLoS One,2017,12(3):e0175035.
[4]王建科，刘辉哲，林鹏，赵航，程悦宁，张淼，郭利，朱洪伟，武华，程世鹏.犬细小病毒新2b型分离株的分离鉴定[J].中国兽医学报，2016,36(5):734-738.
[5] Li C F,Tang J Y,Chen Z Y,Niu G B,Liu G Q. A divergent canine parvovirus type 2c(CPV-2c)isolate circulating in China[J]. Infect Genet Evol,2019,73:242-247.
[6]孙明洁，董国英，张雪竹，胡桂学.犬细小病毒国内外流行病学研究进展[J].中国兽医学报，2019,39(5):1035-1039.
[7] Wang H L,Jin H L,Li Q,Zhao G X,Cheng N,Feng N,Zheng X X,Wang J Z,Zhao Y K,Li L,Cao Z G,Yan F H,Wang L N,Wang T C,Gao Y W,Yang S T, Xia X Z. Isolation and sequence analysis of the complete NS1 and VP2 genes of canine parvovirus from domestic dogs in 2013 and 2014 in China[J]. Arch Virol,2016,161(2):385-393.
[8]张仁舟.犬细小病毒分离鉴定及遗传进化分析[D].中国人民解放军军事医学科学院，2010.
[9]张传鹏，张航，高全新，刘云霞，孙建和，严亚贤.犬细小病毒SH15株的分离鉴定与全基因组分析[J].中国兽医科学，2017,47(2):165-171.
[10]盛宇轩.犬细小病毒吉林株的分离鉴定及VP2-IL-2融合基因的克隆与表达[D].吉林农业大学，2017.
[11]孙明，邓小雨，刘巧荣，马永缨，张丽，卢会英，刘伯华，杨欣艳，陈西钊.犬细小病毒CPV-YH毒株的分离鉴定及其基因组变异分析[J].畜牧兽医学报，2017,48(9):1724-1729.
[12]Zhao H,Wang J,Jiang Y,Cheng Y,Lin P,Zhu H,Han G,Yi L,Zhang S,Guo L,Cheng S. Typing of canine parvovirus strains circulating in North-east China[J]. Transbound Emerg Dis,2015,64(2):495-503.
[13]Han S C,Guo H C,Sun S Q,Shu L,Wei Y Q,Sun D H,Cao S Z,Peng G N,Liu X T. Full-length genomic characterizations of two canine parvoviruses prevalent in Northwest China[J]. Arch Microbiol,2015,197(4):621-626.
[14]Wang J K,Lin P,Zhao H,Cheng Y N,Jiang Z,Zhu H W, Wu H, Cheng S P. Continuing evolution of canine parvovirus in China:Isolation of novel variants with an Ala5Gly mutation in the VP2 protein[J]. Infect Genet Evol,2016,38:73-78.
[15]Zhang R Z,Yang S T,Zhang W,Zhang T,Xie Z J,Feng H,Wang S J,Xia X Z. Phylogenetic analysis of the VP2 Gene of canine parvoviruses circulating in China[J]. Virus Genes,2010,40(3):397-402.
[16]Geng Y F,Guo D H,Li C Q,Wang E Y,Wei S,Wang Z H,Yao S,Zhao X W,Su M J,Wang X Y,Wang J F,Wu R,Feng L,Sun D B. Co-circulation of the rare CPV-2c with unique Gln370Arg substitution,new CPV-2b with unique Thr440Ala substitution,and new CPV-2a with high prevalence and variation in Heilongjiang Province,Northeast China[J/OL]. PLoS One,2015,10(9):e0137288.
基本信息:
DOI：10.13242/j.cnki.bingduxuebao.003637
中图分类号:S852.655
引用信息:
[1]张淮瑜,孙鑫鹏,马小花,等.宁夏地区犬细小病毒的分离鉴定及其VP2基因进化分析[J].病毒学报,2020,36(01):92-99.DOI:10.13242/j.cnki.bingduxuebao.003637.
基金信息:
宁夏自治区高等教育教学改革研究与实践项目(项目号：YJG201837)，题目：宁夏大学兽医硕士人才培养方案改革与实践~~
2020-01-08
2020-01-08
2020-01-08